5-CFDA
- SKU
- C0039
Category: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 5-Carboxyfluorescein diacetate
- 79955-27-4
- CAS-Number
- C25H16O9
- Molecular Formula
- 460.39
- Molecular Weight
Specifications
- Purity
- ≥95% (HPLC)
- Appearance
- White to yellow solid
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO, chloroform
- Fluorescence
- λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)
Downloads
- Safety Data Sheet
- CDX C0039 MSDS.pdf
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- +4°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at +4°C.
- Transportation
- Not dangerous goods
- Description
- Carboxyfluorescein diacetate (CFDA), which was originally used to measure intracellular pH but was soon adapted for use as a cell viability indicator. Upon hydrolysis by intracellular nonspecific esterases, CFDA forms carboxyfluorescein. As compared with fluorescein, carboxyfluorescein contains extra negative charges and is therefore better retained in cells CFDA is moderately permeant to most cell membranes, with uptake greater at pH 6.2 than at pH 7.41. CFDA has been used as a viability probe with a variety of cells, including bacteria, fungi (e.g. Saccharomyces cerevisiae), spermatozoa, natural killer (NK) cells and tumor cells.
- Smiles
- CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(=C3)C(O)=O)C1=CC=C(OC(C)=O)C=C1O2
- InChi Key
- WPUZGNPQMIWOHE-UHFFFAOYSA-N
- References
- (1) J. Immun. Methods 172, 115 (1994) , (2) Anticancer Res. 14, 927 (1994) , (3) Appl. Environ Microbiol. 60, 1467 (1994) , (4) Cytometry 15, 213 (1994) , (5) Biol. Reprod. 34, 127 (1986) , (6) Immun. Lett. 2, 187 (1981)
- InChi
- InChI=1S/C25H16O9/c1-12(26)31-15-4-7-19-21(10-15)33-22-11-16(32-13(2)27)5-8-20(22)25(19)18-6-3-14(23(28)29)9-17(18)24(30)34-25/h3-11H,1-2H3,(H,28,29)
Carboxyfluorescein diacetate (CFDA), which was originally used to measure intracellular pH but was soon adapted for use as a cell viability indicator. Upon hydrolysis by intracellular nonspecific esterases, CFDA forms carboxyfluorescein. As compared with fluorescein, carboxyfluorescein contains extra negative charges and is therefore better retained in cells CFDA is moderately permeant to most cell membranes, with uptake greater at pH 6.2 than at pH 7.41. CFDA has been used as a viability probe with a variety of cells, including bacteria, fungi (e.g. Saccharomyces cerevisiae), spermatozoa, natural killer (NK) cells and tumor cells.