DCFDA
- SKU
- D0063
Category: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 2',7'-Dichlorofluorescein diacetate , Diacetyldichlorofluorescein
- 2044-85-1
- CAS-Number
- C24H14Cl2O7
- Molecular Formula
- 485.27
- Molecular Weight
Specifications
- Purity
- ≥95% (HPLC)
- Appearance
- White powder
- Identity
- 1H-NMR
- Residual Solvent
- Contains ≤ 10% of acetic acid
Properties
- Solvents
- DMSO, acetone
- Melting Point
- 232-234 °C (lit.)
- Fluorescence
- λex 504 nm, λem 524 nm in 0.1 M Tris pH 8.0 (esterase)
Downloads
- Safety Data Sheet
- CDX D0063 MSDS.pdf
- Shipping
- AMBIENT
- Short Term Storage
- RT
- Long Term Storage
- +4°C
- Handling Advice
- Very hygroscopic. Store under inert gas.
- Use / Stability
- Stable for at least 2 years after receipt when stored at +4°C.
- RIDADR
- UN3263
- Transportation
- Not dangerous goods
- Description
- A cell-permeable fluorogenic dye useful for the detection of reactive oxygen species (ROS) and nitric oxide (NO) and for the determination of the degree of overall oxidative stress. The fluorescence of DCFDA indicates oxidation by hydrogen peroxide, peroxynitrite or hydroxyl radical. Superoxide anions can also contribute to DCFDA oxidation albeit at a lesser degree. It measures hydroxyl, peroxyl and other reactive oxygen species (ROS) activity within the cell. After diffusion in to the cell, DCFDA is deacetylated by cellular esterases to a non-fluorescent compound, which is later oxidized by ROS into the highly fluorescent 2’,7’-dichlorofluorescein (DCF). Spectral data: λex=504nm, λem=524nm in 0.1 M Tris pH 8.0 (esterase).
- Smiles
- CC(OC1=C(Cl)C=C2C(OC3=CC(OC(C)=O)=C(Cl)C=C3C24OC(C5=C4C=CC=C5)=O)=C1)=O
- InChi Key
- VQVUBYASAICPFU-UHFFFAOYSA-N
- References
- (1) A.R. Rosenkranz, et al., J. Immunol. Meth. 156, 39 (1992) , (2) T. Takanashi, et al., Invest. Ophthalmol. Vis. Sci. 38, 2721 (1997) , (3) E. Eruslanov & S. Kusmartsev, Methods Mol. Biol. 594, 57 (2010)
- InChi
- InChI=1S/C24H14Cl2O7/c1-11(27)30-21-9-19-15(7-17(21)25)24(14-6-4-3-5-13(14)23(29)33-24)16-8-18(26)22(31-12(2)28)10-20(16)32-19/h3-10H,1-2H3
A cell-permeable fluorogenic dye useful for the detection of reactive oxygen species (ROS) and nitric oxide (NO) and for the determination of the degree of overall oxidative stress. The fluorescence of DCFDA indicates oxidation by hydrogen peroxide, peroxynitrite or hydroxyl radical. Superoxide anions can also contribute to DCFDA oxidation albeit at a lesser degree. It measures hydroxyl, peroxyl and other reactive oxygen species (ROS) activity within the cell. After diffusion in to the cell, DCFDA is deacetylated by cellular esterases to a non-fluorescent compound, which is later oxidized by ROS into the highly fluorescent 2’,7’-dichlorofluorescein (DCF). Spectral data: λex=504nm, λem=524nm in 0.1 M Tris pH 8.0 (esterase).