Specifications
- Purity
- ≥98% (Titration)
- Appearance
- White to off-white to pink powder
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO
- Melting Point
- ≥300 °C
Downloads
- Safety Data Sheet
- CDX T0151 MSDS.pdf
Category: Non Fluorescent Detection & Chromogene Detection
TMBZ is a chromogenic reagent utilized for peroxidase detection. It has been developed as an alternative to benzidine. Although the TMBZ solution is colorless, it turns bluish-green (?max: 655 nm) in the presence of hydrogen peroxide and peroxidase. The structure of this bluish-green complex is thought to be a radical form of two oxidized TMBZ molecules. This peroxidase substrate is used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2M H2SO4 (resulting in a yellow color), and read at 450nm.
Synonyms | 3,3',5,5'-Tetramethylbenzidine dihydrochloride, TMBZ |
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Purity | ≥98% (Titration) |
Appearance | White to off-white to pink powder |
CAS-Number | 64285-73-0 |
Molecular Formula | C16H20N2 . 2HCl |
Molecular Weight | 313.27 |
Identity | 1H-NMR |
Solvents | DMSO |
Melting Point | ≥300 °C |
Smiles | CC1=C(N)C(C)=CC(C2=CC(C)=C(N)C(C)=C2)=C1.Cl |
InChi Key | OBWSOTREAMFOCQ-UHFFFAOYSA-N |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | +4°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
Transportation | Not dangerous goods |
References | (1) V.R. Holland, et al.: Tetrahedron 30, 3299 (1974), (2) P.E. Thomas, et al.: Anal. Biochem. 75, 168 (1976), (3) H.H. Liem, et al.: Anal. Biochem. 98, 388 (1979), (4) E.S. Bos, et al.: J. Immunoassay 2, 187 (1981), (5) K. Suzuki, et al.: Anal. Biochem. 132, 345 (1983), (6) F.H. Pujol, et al.: J. Immunoassay. 14, 21 (1993), (7) F.B. Serrat: Talanta 41, 2091 (1994) |
InChi | InChI=1S/C16H20N2.ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14 |
Quantity | 1 g, 5 g, Bulk |