Specifications
- Purity
- ≥98% (HPLC)
- Appearance
- Faint green to yellow powder
- Identity
- 1H-NMR
Properties
- Solvents
- water (10mg/ml) or DMSO (10mg/ml)
Documentation
- Safety Data Sheet (SDS)
- CDX A0591 SDS_Vs1.1.pdf
Available from stock
ABTS diammonium salt
- SKU
- A0591
Category: Fluorescent Detection
- Synonyms
- AzBTS-(NH4)2, 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, Diammonium 2,2?-azinobis(3-ethyl-6-benzothiazolinesulfonate)
- 30931-67-0
- CAS-Number
- C18H16N4O6S4 . 2NH4
- Molecular Formula
- 548.68
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- RT
- Long Term Storage
- +4°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at +4°C.
- Transportation
- Not dangerous goods
- Description
- ABTS (2,2' -Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid), diammonium salt) is a very sensitive chromogenic peroxidase substrate (including horseradish peroxidase (HRP)) widely used for ELISA applications. The substrate yields a water-soluble green colored product that can be quantified by colorimetric detection at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). It has commonly been used to assess antioxidant capacity in the Trolox equivalent antioxidant capacity (TEAC) assay. It has a blue color in the presence of sodium persulfate or metmyoglobin but decolorizes upon incubation with antioxidants, and the antioxidant capacity can be determined spectrophotometrically. ABTS is significantly used by the food industry, to measure the antioxidant capacities within different foods. ABTS is also used to monitor glucose concentrations in different solutions including blood serum and as a safe alternative to DAB for the enhancement of blood fingerprints.
- Smiles
- C(C)N1+W2:Y46C=2C(SC1=NN=C3N(CC)C=4C(S3)=CC(S(=O)(=O)O)=CC4)=CC(S(=O)(=O)O)=CC2.N
- InChi Key
- OHDRQQURAXLVGJ-AXMZSLBLSA-N
- References
- (1) R.E. Childs & W.G. Bardsley, Biochem. J. 145, 93 (1975) , (2) N.P. Groome, J. Clin. Chem. Clin. Biochem. 18, 345 (1980) , (3) E. Engvall, Meth. Enzymol 70, 419 (1980) , (4) E. Al-Kaissi & A. Mostratos, J. Immunol. Methods. 58, 127 (1983) , (5) A. Szutowicz, et al., Anal. Biochem. 138, 86 (1984) , (6) N.J. Miller & C.A. Rice-Evans, Free Radic. Res. 26, 195 (1997) , (7) J.P. Caldwell, et al., J. Forensic Sci. 45, 785 (2000) , (8) D. Huang, et al., J. Agric. Food Chem. 53, 1841 (2005) , (9) A. Reyna-Bello, et al., J. Immunoassay Immunochem. 28, 1 (2007) , (10) R.B. Walker & J.D. Everette, J. Agric. Food Chem. 57, 1156 (2009) , (11) A.A. Salem, et al., Anal. Chem. Insights 6, 37 (2011) , (12) Y. Li, et al., Spectrochim. Acta A Mol. Biomol. Spectrosc. 173, 880 (2017) , (13) A. Liberato, et al., Inorg. Chem. 57, 14471 (2018)
- InChi
- InChI=1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18,,/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28),2*1H3
ABTS (2,2′ -Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid), diammonium salt) is a very sensitive chromogenic peroxidase substrate (including horseradish peroxidase (HRP)) widely used for ELISA applications. The substrate yields a water-soluble green colored product that can be quantified by colorimetric detection at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). It has commonly been used to assess antioxidant capacity in the Trolox equivalent antioxidant capacity (TEAC) assay. It has a blue color in the presence of sodium persulfate or metmyoglobin but decolorizes upon incubation with antioxidants, and the antioxidant capacity can be determined spectrophotometrically. ABTS is significantly used by the food industry, to measure the antioxidant capacities within different foods. ABTS is also used to monitor glucose concentrations in different solutions including blood serum and as a safe alternative to DAB for the enhancement of blood fingerprints.
Additional information
| Synonyms | AzBTS-(NH4)2, 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, Diammonium 2,2?-azinobis(3-ethyl-6-benzothiazolinesulfonate) |
|---|---|
| Purity | ≥98% (HPLC) |
| Appearance | Faint green to yellow powder |
| CAS-Number | 30931-67-0 |
| Molecular Formula | C18H16N4O6S4 . 2NH4 |
| Molecular Weight | 548.68 |
| Identity | 1H-NMR |
| Solvents | water (10mg/ml) or DMSO (10mg/ml) |
| Smiles | C(C)N1+W2:Y46C=2C(SC1=NN=C3N(CC)C=4C(S3)=CC(S(=O)(=O)O)=CC4)=CC(S(=O)(=O)O)=CC2.N |
| InChi Key | OHDRQQURAXLVGJ-AXMZSLBLSA-N |
| Shipping | AMBIENT |
| Short Term Storage | RT |
| Long Term Storage | +4°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Transportation | Not dangerous goods |
| References | (1) R.E. Childs & W.G. Bardsley, Biochem. J. 145, 93 (1975), (2) N.P. Groome, J. Clin. Chem. Clin. Biochem. 18, 345 (1980), (3) E. Engvall, Meth. Enzymol 70, 419 (1980), (4) E. Al-Kaissi & A. Mostratos, J. Immunol. Methods. 58, 127 (1983), (5) A. Szutowicz, et al., Anal. Biochem. 138, 86 (1984), (6) N.J. Miller & C.A. Rice-Evans, Free Radic. Res. 26, 195 (1997), (7) J.P. Caldwell, et al., J. Forensic Sci. 45, 785 (2000), (8) D. Huang, et al., J. Agric. Food Chem. 53, 1841 (2005), (9) A. Reyna-Bello, et al., J. Immunoassay Immunochem. 28, 1 (2007), (10) R.B. Walker & J.D. Everette, J. Agric. Food Chem. 57, 1156 (2009), (11) A.A. Salem, et al., Anal. Chem. Insights 6, 37 (2011), (12) Y. Li, et al., Spectrochim. Acta A Mol. Biomol. Spectrosc. 173, 880 (2017), (13) A. Liberato, et al., Inorg. Chem. 57, 14471 (2018) |
| InChi | InChI=1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18,,/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28),2*1H3 |
| Quantity | 1 g, 5 g, Bulk |
