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Acridine orange 10-nonyl bromide

SKU: A0103

Categories: Fluorescent Detection, Fluorescent Detection

Synonyms: 10-Nonylacridine orange bromide , 3,6-Bis(dimethylamino)-10-nonylacridinium bromide , 10-nonyl Acridine Orange , NAO; 75168-11-5
CAS-Number: C₂₆H₃₈BrN₃
Molecular Formula: 472.50
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥90% (HPLC)
Appearance: Dark red powder
Identity: 1H-NMR

Properties

Solvents: Soluble in DMF, ethanol, DMSO or methanol.
Melting Point: ≥250 °C (lit.)
Fluorescence: λex 494 nm, λem 519 nm in methanol

Shipping: AMBIENT
Short Term Storage: RT
Long Term Storage: +4°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at +4°C.

Transportation: Not dangerous goods

Description: Acridine orange 10-nonyl is a green fluorescent mitochondrial dye. The dye incorporates into the cardiolipin-rich inner mitochondrial membrane. Acridine orange 10-nonyl has been used to study multidrug resistance and to measure changes in mitochondrial mass during apoptosis. Acridine orange 10-nonyl has been used to quantify cardiolipin content and mitochondrial mass in isolated mitochondria and cellular systems. Spectral Data: λEx/λEm (MeOH) = 495/522nm.
Smiles: CN(C)C1=CC2=[N+](CCCCCCCCC)C3=CC(N(C)C)=CC=C3C=C2C=C1.[Br-]
InChi Key: DRBHTUDHPPBMCD-UHFFFAOYSA-M
References: (1) M.H. Ratinaud, et al., Cytometry 9, 206 (1988) , (2) A. Maftah, et al., FEBS Letters 260, 236 (1990) , (3) D. Kessel, et al., Cancer Res. 51, 4665 (1991) , (4) J.M. Petit, et al., Eur. J. Biochem. 209, 267 (1992) , (5) A. Cossarizza, et al., Exp. Cell Res. 214, 323 (1994) , (6) E. Mileykovskaya & W. Dowhan, J. Bacteriol. 182, 1172 (2000) , (7) J.F. Keij, et al., Cytometry 39, 203 (2000) , (8) J. Jacobson, et al., J. Neurochem. 82, 224 (2002) , (9) M.I. Garcia Fernandez, et al., Anal. Biochem. 328, 174 (2004)
InChi: InChI=1S/C26H38N3.BrH/c1-6-7-8-9-10-11-12-17-29-25-19-23(27(2)3)15-13-21(25)18-22-14-16-24(28(4)5)20-26(22)29;/h13-16,18-20H,6-12,17H2,1-5H3;1H/q+1;/p-1

Acridine orange 10-nonyl is a green fluorescent mitochondrial dye. The dye incorporates into the cardiolipin-rich inner mitochondrial membrane. Acridine orange 10-nonyl has been used to study multidrug resistance and to measure changes in mitochondrial mass during apoptosis. Acridine orange 10-nonyl has been used to quantify cardiolipin content and mitochondrial mass in isolated mitochondria and cellular systems. Spectral Data: λEx/λEm (MeOH) = 495/522nm.

SKU: A0103 Categories: Fluorescent Detection, Fluorescent Detection

Additional information

Additional information

Synonyms	10-Nonylacridine orange bromide, 3,6-Bis(dimethylamino)-10-nonylacridinium bromide, 10-nonyl Acridine Orange, NAO
Purity	≥90% (HPLC)
Appearance	Dark red powder
CAS-Number	75168-11-5
Molecular Formula	C26H38BrN3
Molecular Weight	472.50
Identity	1H-NMR
Solvents	Soluble in DMF, ethanol, DMSO or methanol.
Melting Point	≥250 °C (lit.)
Fluorescence	λex 494 nm, λem 519 nm in methanol
Smiles	CN(C)C1=CC2=[N+](CCCCCCCCC)C3=CC(N(C)C)=CC=C3C=C2C=C1.[Br-]
InChi Key	DRBHTUDHPPBMCD-UHFFFAOYSA-M
Shipping	AMBIENT
Short Term Storage	RT
Long Term Storage	+4°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at +4°C.
Transportation	Not dangerous goods
References	(1) M.H. Ratinaud, et al., Cytometry 9, 206 (1988), (2) A. Maftah, et al., FEBS Letters 260, 236 (1990), (3) D. Kessel, et al., Cancer Res. 51, 4665 (1991), (4) J.M. Petit, et al., Eur. J. Biochem. 209, 267 (1992), (5) A. Cossarizza, et al., Exp. Cell Res. 214, 323 (1994), (6) E. Mileykovskaya & W. Dowhan, J. Bacteriol. 182, 1172 (2000), (7) J.F. Keij, et al., Cytometry 39, 203 (2000), (8) J. Jacobson, et al., J. Neurochem. 82, 224 (2002), (9) M.I. Garcia Fernandez, et al., Anal. Biochem. 328, 174 (2004)
InChi	InChI=1S/C26H38N3.BrH/c1-6-7-8-9-10-11-12-17-29-25-19-23(27(2)3)15-13-21(25)18-22-14-16-24(28(4)5)20-26(22)29;/h13-16,18-20H,6-12,17H2,1-5H3;1H/q+1;/p-1
Quantity	100 mg, Bulk