Specifications
- Purity
- ≥98% (HPLC)
- Appearance
- Light yellow to orange powder
- Identity
- 1H-NMR
Properties
- Solvents
- Soluble in DMF, DMSO, ethanol or methanol (all 10mg/ml). Insoluble in water.
Documentation
- Safety Data Sheet (SDS)
- CDX A0054 SDS_Vs1.1.pdf
Available from stock
Aminophenyl-fluorescein
- SKU
- A0054
Category: Fluorescent Detection
- Synonyms
- APF, 3'-p-(Aminophenyl) fluorescein, 2-[6-(4'-Amino)phenoxy-3H-xanthen-3-on-9-yl]benzoic acid, 3'-(4-Aminophenoxy)-6'-hydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
- 359010-70-1
- CAS-Number
- C26H17NO5
- Molecular Formula
- 423.42
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Hazard statements
- H319
- Precautionary statements
- P264 - P280 - P305 + P351 + P338 - P337 + P313
- GHS Symbol
- GHS07
- Signal word
- Warning
- Transportation
- Not dangerous goods
- Description
- APF is a cell-permeable fluorogenic probe for highly reactive radicals. It has low intrinsic fluorescence, however, upon oxidation by hydroxyl radical, hypochlorite ion, and certain peroxidase intermediates, it is converted to the highly fluorescent molecule fluorescein, facilitating the detection of highly reactive biological radicals. APF can be used to detect intracellular reactive oxygen species (ROS). APF is not oxidized by nitric oxide (NO), hydrogen peroxide (H2O2) or other oxidants. Spectral Data of Fluorescein: Ex/Em maxima 490/515 nm.
- Smiles
- O=C1OC2(C3=CC=C(O)C=C3OC4=CC(OC5=CC=C(N)C=C5)=CC=C42)C=6C=CC=CC16
- InChi Key
- SKRNNOWWEXPZBB-UHFFFAOYSA-N
- References
- (1) K. Setsukinai, et al., J. Biol. Chem. 278, 3170 (2003) , (2) J. Flemmig, et al., J. Biol. Chem. 287, 27913 (2012) , (3) T.K. Kim, et al., Chemosphere 208, 441 (2018) , (4) H. Tamura, et al., Neurotoxicol. 95, 173 (2023)
- InChi
- InChI=1S/C26H17NO5/c27-15-5-8-17(9-6-15)30-18-10-12-22-24(14-18)31-23-13-16(28)7-11-21(23)26(22)20-4-2-1-3-19(20)25(29)32-26/h1-14,28H,27H2
APF is a cell-permeable fluorogenic probe for highly reactive radicals. It has low intrinsic fluorescence, however, upon oxidation by hydroxyl radical, hypochlorite ion, and certain peroxidase intermediates, it is converted to the highly fluorescent molecule fluorescein, facilitating the detection of highly reactive biological radicals. APF can be used to detect intracellular reactive oxygen species (ROS). APF is not oxidized by nitric oxide (NO), hydrogen peroxide (H2O2) or other oxidants. Spectral Data of Fluorescein: Ex/Em maxima 490/515 nm.
Additional information
| Synonyms | APF, 3'-p-(Aminophenyl) fluorescein, 2-[6-(4'-Amino)phenoxy-3H-xanthen-3-on-9-yl]benzoic acid, 3'-(4-Aminophenoxy)-6'-hydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one |
|---|---|
| Purity | ≥98% (HPLC) |
| Appearance | Light yellow to orange powder |
| CAS-Number | 359010-70-1 |
| Molecular Formula | C26H17NO5 |
| Molecular Weight | 423.42 |
| Identity | 1H-NMR |
| Solvents | Soluble in DMF, DMSO, ethanol or methanol (all 10mg/ml). Insoluble in water. |
| Smiles | O=C1OC2(C3=CC=C(O)C=C3OC4=CC(OC5=CC=C(N)C=C5)=CC=C42)C=6C=CC=CC16 |
| InChi Key | SKRNNOWWEXPZBB-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Hazard statements | H319 |
| Precautionary statements | P264 – P280 – P305 + P351 + P338 – P337 + P313 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) K. Setsukinai, et al., J. Biol. Chem. 278, 3170 (2003), (2) J. Flemmig, et al., J. Biol. Chem. 287, 27913 (2012), (3) T.K. Kim, et al., Chemosphere 208, 441 (2018), (4) H. Tamura, et al., Neurotoxicol. 95, 173 (2023) |
| InChi | InChI=1S/C26H17NO5/c27-15-5-8-17(9-6-15)30-18-10-12-22-24(14-18)31-23-13-16(28)7-11-21(23)26(22)20-4-2-1-3-19(20)25(29)32-26/h1-14,28H,27H2 |
| Quantity | 1 mg, 5 mg, Bulk |
