Specifications
- Purity
- ≥80% (Dye content)
- Appearance
- Dark green to black powder
Properties
- Solvents
- Slightly soluble in water or DMSO (0.1 - 1mg/ml).
- Melting Point
- 290 °C (dec.) (lit.)
Documentation
- Safety Data Sheet (SDS)
- CDX A0673 SDS_Vs1.1.pdf
Available from stock
Azure A chloride
- SKU
- A0673
Category: Non Fluorescent Detection & Chromogene Detection
- Synonyms
- Azure A dye, 3-Amino-7-(dimethylamino)phenazathionium chloride, N,N-Dimethylthionine, C.I. 52005, Azure Blue Cert
- 531-53-3
- CAS-Number
- C14H14ClN3S
- Molecular Formula
- 291.80
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- RT
- Long Term Storage
- RT
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at RT.
- Transportation
- Not dangerous goods
- Description
- Azure A chloride is a cationic thiazine dye widely used in histology and microbiology for staining cellular structures. It displays an absorbance maximum between 620-634 nm in water. It exhibits strong affinity for acidic tissue components such as nucleic acids, making it useful in procedures that highlight nuclei and other basophilic elements. It is commonly used in combination staining methods (e.g., Romanowsky-type stains) for blood smears and tissue sections, providing clear contrast of cellular morphology. Azure A Chloride is commonly used as a nucleic acid (DNA/RNA) stain. It is a basic (cationic) dye that binds strongly to negatively charged nucleic acids, meaning it can stain DNA (especially in nuclei) and RNA (often even more strongly than DNA in some protocols). Azure A has also been used as a starting material in the synthesis of DNA damage-sensing electrochemical probes and NADH-detecting electrodes.
- Smiles
- [Cl-].N=1C2=CC=C(N)C=C2[S+]=C3C=C(C=CC13)N(C)C
- InChi Key
- PGWTYMLATMNCCZ-UHFFFAOYSA-M
- References
- (1) A. Wollin & L.B. Jaques, Can. J. Physiol. Pharmacol. 50, 65 (1972) , (2) P.J. Bennion, et al., Stain. Technol. 50, 307 (1975) , (3) C. Doriguzzi, et al., Histochemistry 79, 289 (1983) , (4) P. Chieco, et al., Histochem. J. 25, 569 (1993) , (5) V. Grill, et al., Biotech. Histochem. 70, 75 (1975) , (6) R.W. Sabnis, Handbook of biological dyes and stains (2010) , (7) M. Revenga-Parra, et al., Anal. Chim. Acta 747, 84 (2012) , (8) P. Paul & G. Suresh Kumar, Spectrochim. Acta A Mol. Biomol. Spectrosc. 107, 303 (2013) , (9) A. Porfireva, et al., Sensors 21, 2949 (2021)
- InChi
- InChI=1S/C14H14N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12,/h3-8H,15H2,1-2H3,1H/q+1,/p-1
Azure A chloride is a cationic thiazine dye widely used in histology and microbiology for staining cellular structures. It displays an absorbance maximum between 620-634 nm in water. It exhibits strong affinity for acidic tissue components such as nucleic acids, making it useful in procedures that highlight nuclei and other basophilic elements. It is commonly used in combination staining methods (e.g., Romanowsky-type stains) for blood smears and tissue sections, providing clear contrast of cellular morphology. Azure A Chloride is commonly used as a nucleic acid (DNA/RNA) stain. It is a basic (cationic) dye that binds strongly to negatively charged nucleic acids, meaning it can stain DNA (especially in nuclei) and RNA (often even more strongly than DNA in some protocols). Azure A has also been used as a starting material in the synthesis of DNA damage-sensing electrochemical probes and NADH-detecting electrodes.
Additional information
| Synonyms | Azure A dye, 3-Amino-7-(dimethylamino)phenazathionium chloride, N,N-Dimethylthionine, C.I. 52005, Azure Blue Cert |
|---|---|
| Purity | ≥80% (Dye content) |
| Appearance | Dark green to black powder |
| CAS-Number | 531-53-3 |
| Molecular Formula | C14H14ClN3S |
| Molecular Weight | 291.80 |
| Solvents | Slightly soluble in water or DMSO (0.1 – 1mg/ml). |
| Melting Point | 290 °C (dec.) (lit.) |
| Smiles | [Cl-].N=1C2=CC=C(N)C=C2[S+]=C3C=C(C=CC13)N(C)C |
| InChi Key | PGWTYMLATMNCCZ-UHFFFAOYSA-M |
| Shipping | AMBIENT |
| Short Term Storage | RT |
| Long Term Storage | RT |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at RT. |
| Transportation | Not dangerous goods |
| References | (1) A. Wollin & L.B. Jaques, Can. J. Physiol. Pharmacol. 50, 65 (1972), (2) P.J. Bennion, et al., Stain. Technol. 50, 307 (1975), (3) C. Doriguzzi, et al., Histochemistry 79, 289 (1983), (4) P. Chieco, et al., Histochem. J. 25, 569 (1993), (5) V. Grill, et al., Biotech. Histochem. 70, 75 (1975), (6) R.W. Sabnis, Handbook of biological dyes and stains (2010), (7) M. Revenga-Parra, et al., Anal. Chim. Acta 747, 84 (2012), (8) P. Paul & G. Suresh Kumar, Spectrochim. Acta A Mol. Biomol. Spectrosc. 107, 303 (2013), (9) A. Porfireva, et al., Sensors 21, 2949 (2021) |
| InChi | InChI=1S/C14H14N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12,/h3-8H,15H2,1-2H3,1H/q+1,/p-1 |
| Quantity | 50 g, 100 g, Bulk |
| cdx-Absorbtion max. | 627 – 633 nm (in water) |
