Available from stock

Azure B

SKU: A0164

Category: Building Blocks, Intermediates, Reagents

Synonyms: C.I. 52010, Trimethylthionine, Azure I, Methylene azure, 3-Methylamino-7-dimethylaminophenazathionium chloride; 531-55-5
CAS-Number: C₁₅H₁₆ClN₃S
Molecular Formula: 305.83
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥80% (Dye content)
Appearance: Dark green powder
Identity: 1H-NMR

Properties

Solvents: Soluble in water (1 mg/ml). Sparingly soluble in ethanol.
Melting Point: 205-210 °C (dec.) (lit.)
Fluorescence: λex 648 in ethanol

Documentation

Safety Data Sheet (SDS): CDX A0164 SDS_Vs1.1.pdf

Shipping: AMBIENT
Short Term Storage: RT
Long Term Storage: RT
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at RT.

Hazard statements: H302
Precautionary statements: P264 - P270 - P301 + P312 - P501
GHS Symbol: GHS07
Signal word: Warning
Transportation: Not dangerous goods

Description: Azure B is a cationic thiazine dye widely used in histology, cytology and microbiology for biological staining applications. It is commonly employed as a component of Romanowsky-type stains, including Giemsa and related staining systems, where it contributes to the selective coloration of cellular structures and nucleic acids. Known for its strong chromatic properties and reliable staining performance, Azure B is used in the visualization of blood smears, tissue sections, and microbial preparations. Its compatibility with other thiazine dyes, such as methylene blue and Azure A, makes it an important reagent for differential staining protocols in diagnostic and research laboratories. Spectral Data: ?ex 648 (in ethanol). Azure B is also a high-potency, selective and reversible inhibitor of monoamine oxidases (MAO)-A. It is the main Metabolite of methylene blue.
Smiles: C[N+](C)=C(C=C1)C=C(C1=N2)SC3=C2C=CC(NC)=C3.[Cl-]
InChi Key: DNDJEIWCTMMZBX-UHFFFAOYSA-N
References: (1) W. Lohr, et al., Stain Technol. 50, 149 (1975) , (2) M.K. Dutt, Microsc. Acta. 83, 403 (1980) , (3) D.H. Wittekind, et al., Br. J. Haematol. 51, 391 (1982) , (4) D. Wittekind & E. Schulte, Clin. Lab. Haematol. 9, 395 (1987) , (5) F.S. Archibald, Appl. Environ. Microbiol. 58, 3110 (1992) , (6) B. Koch, et al., Biotech. Histochem. 70, 267 (1995) , (7) D.P. Penney, et al., Biotech. Histochem. 77, 237 (2002) , (8) A. Petzer, et al., Toxicol. Appl. Pharmacol. 258, 403 (2012) , (9) A. Delport, et al., Life Sci. 117, 56 (2014) , (10) L.O. Kostjukova, et al., J. Mol. Graph. Moδ 107, 107964 (2021)
InChi: InChI=1S/C15H15N3S.ClH/c1-16-10-4-6-12-14(8-10)19-15-9-11(18(2)3)5-7-13(15)17-12,/h4-9H,1-3H3,1H

Azure B is a cationic thiazine dye widely used in histology, cytology and microbiology for biological staining applications. It is commonly employed as a component of Romanowsky-type stains, including Giemsa and related staining systems, where it contributes to the selective coloration of cellular structures and nucleic acids. Known for its strong chromatic properties and reliable staining performance, Azure B is used in the visualization of blood smears, tissue sections, and microbial preparations. Its compatibility with other thiazine dyes, such as methylene blue and Azure A, makes it an important reagent for differential staining protocols in diagnostic and research laboratories. Spectral Data: ?ex 648 (in ethanol). Azure B is also a high-potency, selective and reversible inhibitor of monoamine oxidases (MAO)-A. It is the main Metabolite of methylene blue.

SKU: A0164 Category: Building Blocks, Intermediates, Reagents

Additional information

Additional information

Synonyms	C.I. 52010, Trimethylthionine, Azure I, Methylene azure, 3-Methylamino-7-dimethylaminophenazathionium chloride
Purity	≥80% (Dye content)
Appearance	Dark green powder
CAS-Number	531-55-5
Molecular Formula	C15H16ClN3S
Molecular Weight	305.83
Identity	1H-NMR
Solvents	Soluble in water (1 mg/ml). Sparingly soluble in ethanol.
Melting Point	205-210 °C (dec.) (lit.)
Fluorescence	λex 648 in ethanol
Smiles	C[N+](C)=C(C=C1)C=C(C1=N2)SC3=C2C=CC(NC)=C3.[Cl-]
InChi Key	DNDJEIWCTMMZBX-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	RT
Long Term Storage	RT
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at RT.
Hazard statements	H302
Precautionary statements	P264 – P270 – P301 + P312 – P501
GHS Symbol	GHS07
Signal word	Warning
Transportation	Not dangerous goods
References	(1) W. Lohr, et al., Stain Technol. 50, 149 (1975), (2) M.K. Dutt, Microsc. Acta. 83, 403 (1980), (3) D.H. Wittekind, et al., Br. J. Haematol. 51, 391 (1982), (4) D. Wittekind & E. Schulte, Clin. Lab. Haematol. 9, 395 (1987), (5) F.S. Archibald, Appl. Environ. Microbiol. 58, 3110 (1992), (6) B. Koch, et al., Biotech. Histochem. 70, 267 (1995), (7) D.P. Penney, et al., Biotech. Histochem. 77, 237 (2002), (8) A. Petzer, et al., Toxicol. Appl. Pharmacol. 258, 403 (2012), (9) A. Delport, et al., Life Sci. 117, 56 (2014), (10) L.O. Kostjukova, et al., J. Mol. Graph. Moδ 107, 107964 (2021)
InChi	InChI=1S/C15H15N3S.ClH/c1-16-10-4-6-12-14(8-10)19-15-9-11(18(2)3)5-7-13(15)17-12,/h4-9H,1-3H3,1H
Quantity	5 g, 25 g, Bulk