Available from stock

BAPTA tetrasodium salt

SKU: A0119

Category: Fluorescent Detection

Synonyms: BAPTA tetrasodium salt, 1,2- bis(o-Aminophenoxy)ethane-N,N,N?,N?-tetraacetic acid 4Na; 126824-24-6
CAS-Number: C₂₂H₂₀N₂Na₄O₁₀
Molecular Formula: 564.36
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥95% (HPLC)
Appearance: White to faint beige powder
Identity: 1H-NMR

Properties

Solvents: Soluble in water (10 mg/ml).

Documentation

Safety Data Sheet (SDS): CDX A0119 SDS_Vs1.1.pdf

Shipping: AMBIENT
Short Term Storage: +4°C
Long Term Storage: -20°C
Handling Advice: Protect from light and air. Store under inert gas.
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.

Transportation: Not dangerous goods

Description: BAPTA is a membrane-impermeable highly selective calcium chelator and is useful for manipulating extracellular Ca2+ levels, with 10^5-fold greater affinity for Ca2+ than for Mg2+. The presence of four carboxylic acid functional groups allows for the binding of two calcium ions. BAPTA and its derivatives can be used as calcium indicators, since the absorption maximum for BAPTA changes when it is complexed with calcium (absorption maxima free/complexed = 254/274 nm, emission maxima free/complexed = 363/363 nm). Key advantages of BAPTA include relative insensitivity toward intracellular pH change and fast release of calcium. Its rapid calcium-binding kinetics and high affinity for Ca2+ make it an essential tool for studying calcium-dependent signaling pathways, enzyme activity, neurotransmitter release and other cellular processes.
Smiles: [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)c1ccccc1OCCOc2ccccc2N(CC([O-])=O)CC([O-])=O
InChi Key: QEANQYJKLAFALF-UHFFFAOYSA-J
References: (1) R.Y. Tsien, et al., Biochemistry. 19, 2396 (1980) , (2) R.Y. Tsien, et al., Annu. Rev. Biophys. Bioeng. 12, 91 (1983) , (3) G. Grynkiewicz, et al., J. Biol. Chem. 260, 3440 (1985) , (4) H. Kijima, et al., J. Physiol. 403, 135 (1988) , (5) N. Tanabe, et al., Neurosci. Lett. 92, 52 (1988) , (6) R.C. Hardie, Cell Calcium. 38, 547 (2005) , (7) R.W. Sabnis, Handbook of biological dyes and stains (2010)
InChi: InChI=1S/C22H24N2O10.4Na/c25-19(26)11-23(12-20(27)28)15-5-1-3-7-17(15)33-9-10-34-18-8-4-2-6-16(18)24(13-21(29)30)14-22(31)32,,,,/h1-8H,9-14H2,(H,25,26)(H,27,28)(H,29,30)(H,31,32),,,,/q,4*+1/p-4

BAPTA is a membrane-impermeable highly selective calcium chelator and is useful for manipulating extracellular Ca2+ levels, with 10^5-fold greater affinity for Ca2+ than for Mg2+. The presence of four carboxylic acid functional groups allows for the binding of two calcium ions. BAPTA and its derivatives can be used as calcium indicators, since the absorption maximum for BAPTA changes when it is complexed with calcium (absorption maxima free/complexed = 254/274 nm, emission maxima free/complexed = 363/363 nm). Key advantages of BAPTA include relative insensitivity toward intracellular pH change and fast release of calcium. Its rapid calcium-binding kinetics and high affinity for Ca2+ make it an essential tool for studying calcium-dependent signaling pathways, enzyme activity, neurotransmitter release and other cellular processes.

SKU: A0119 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	BAPTA tetrasodium salt, 1,2- bis(o-Aminophenoxy)ethane-N,N,N?,N?-tetraacetic acid 4Na
Purity	≥95% (HPLC)
Appearance	White to faint beige powder
CAS-Number	126824-24-6
Molecular Formula	C22H20N2Na4O10
Molecular Weight	564.36
Identity	1H-NMR
Solvents	Soluble in water (10 mg/ml).
Smiles	[Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)c1ccccc1OCCOc2ccccc2N(CC([O-])=O)CC([O-])=O
InChi Key	QEANQYJKLAFALF-UHFFFAOYSA-J
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and air. Store under inert gas.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Transportation	Not dangerous goods
References	(1) R.Y. Tsien, et al., Biochemistry. 19, 2396 (1980), (2) R.Y. Tsien, et al., Annu. Rev. Biophys. Bioeng. 12, 91 (1983), (3) G. Grynkiewicz, et al., J. Biol. Chem. 260, 3440 (1985), (4) H. Kijima, et al., J. Physiol. 403, 135 (1988), (5) N. Tanabe, et al., Neurosci. Lett. 92, 52 (1988), (6) R.C. Hardie, Cell Calcium. 38, 547 (2005), (7) R.W. Sabnis, Handbook of biological dyes and stains (2010)
InChi	InChI=1S/C22H24N2O10.4Na/c25-19(26)11-23(12-20(27)28)15-5-1-3-7-17(15)33-9-10-34-18-8-4-2-6-16(18)24(13-21(29)30)14-22(31)32,,,,/h1-8H,9-14H2,(H,25,26)(H,27,28)(H,29,30)(H,31,32),,,,/q,4*+1/p-4
Quantity	1 g, Bulk