Specifications
- Purity
- ≥80.0% (dye content)
- Appearance
- Green powder
- UV/Vis
- 545 - 555 nm
Properties
- Solvents
- Soluble in water, methanol or DMSO.
- Melting Point
- 205° C
Available from stock
Basic Fuchsin
- SKU
- F0102
Category: Fluorescent Detection
- Synonyms
- C.I. Basic Violet 14, Fuchsine Basic, Basic Magenta, Rosaniline, C.I. 42510, Aizen Magenta
- 632-99-5
- CAS-Number
- C20H20ClN3
- Molecular Formula
- 337.85
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- RT
- Long Term Storage
- RT
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at RT.
- Hazard statements
- H351
- Precautionary statements
- P201 - P308 + P313
- GHS Symbol
- GHS08
- Signal word
- Warning
- Transportation
- Not dangerous goods
- Description
- Basic Fuchsin is a triphenylmethane dye renowned for its intense magenta color. It is primarily used in microbiological and histological staining as it has a strong affinity for nucleic acids and cellular components, making it invaluable in the visualization of cell structures under a microscope. The mechanism of action of Basic Fuchsin involves its basic properties, which allow it to bind to acidic components of cells, particularly nucleic acids, through electrostatic interactions. In research, Basic Fuchsin is extensively used in the Gram staining procedure, a fundamental technique for bacterial classification and identification. In this process, it serves as a primary stain that colors all cells. However, its retention in Gram-positive bacteria, due to their thicker peptidoglycan layer, contrasts sharply with its removal from Gram-negative bacteria during a decolorization step, demonstrating its selective staining capabilities. Additionally, Basic Fuchsin is used in other histological stains, such as the Schiff reagent for the periodic acid-Schiff reaction, which is essential for identifying polysaccharides such as glycogen in tissues and is also used for staining of acid-fast bacteria (AFB). Through these applications, Basic Fuchsin continues to be a pivotal tool in both clinical diagnostics and biological research, providing critical insights into microbial morphology and cellular biochemistry.
- Smiles
- NC1=C(C)C=C(/C(C2=CC=C(N)C=C2)=C3C=CC(C=C/3)=N)C=C1.Cl
- InChi Key
- NIKFYOSELWJIOF-SVFFXJIWSA-N
- References
- (1) E. Adams; Stain Technol. 50, 227 (1975) , (2) D.K. Cooper; Histochem. J. 9, 285 (1977) , (3) K. Pihlman & E. Linder; Histochem. 79, 157 (1983) , (4) T.J. Beveridge; Biotech. Histochem. 76, 111 (2001) , (5) T. Frisch, et al.; Eur. Arch. Otorhinolaryngol. 258, 55 (2001) , (6) R. Coico, et al.; Curr. Protoc. Microbiol. Appendix 3-3C (2005) , (7) C. Gordon, et al.; Int. J. Tuberc. Lung. Dis. 13, 130 (2009) , (8) M. Chen, et al.; Bioanalysis 5, 1545 (2013) , (9) B. Pathrose, et al.; Spectrochim. Acta A Mol. Biomol. Spectrosc. 128, 522 (2014) , (10) B. Pathrose, et al.; J. Fluoresc. 24, 895 (2014)
- InChi
- InChI=1S/C20H19N3.ClH/c1-13-12-16(6-11-19(13)23)20(14-2-7-17(21)8-3-14)15-4-9-18(22)10-5-15;/h2-12,21H,22-23H2,1H3;1H
Basic Fuchsin is a triphenylmethane dye renowned for its intense magenta color. It is primarily used in microbiological and histological staining as it has a strong affinity for nucleic acids and cellular components, making it invaluable in the visualization of cell structures under a microscope. The mechanism of action of Basic Fuchsin involves its basic properties, which allow it to bind to acidic components of cells, particularly nucleic acids, through electrostatic interactions. In research, Basic Fuchsin is extensively used in the Gram staining procedure, a fundamental technique for bacterial classification and identification. In this process, it serves as a primary stain that colors all cells. However, its retention in Gram-positive bacteria, due to their thicker peptidoglycan layer, contrasts sharply with its removal from Gram-negative bacteria during a decolorization step, demonstrating its selective staining capabilities. Additionally, Basic Fuchsin is used in other histological stains, such as the Schiff reagent for the periodic acid-Schiff reaction, which is essential for identifying polysaccharides such as glycogen in tissues and is also used for staining of acid-fast bacteria (AFB). Through these applications, Basic Fuchsin continues to be a pivotal tool in both clinical diagnostics and biological research, providing critical insights into microbial morphology and cellular biochemistry.
Additional information
| Synonyms | C.I. Basic Violet 14, Fuchsine Basic, Basic Magenta, Rosaniline, C.I. 42510, Aizen Magenta |
|---|---|
| Purity | ≥80.0% (dye content) |
| Appearance | Green powder |
| CAS-Number | 632-99-5 |
| Molecular Formula | C20H20ClN3 |
| Molecular Weight | 337.85 |
| Solvents | Soluble in water, methanol or DMSO. |
| Melting Point | 205° C |
| Smiles | NC1=C(C)C=C(/C(C2=CC=C(N)C=C2)=C3C=CC(C=C/3)=N)C=C1.Cl |
| InChi Key | NIKFYOSELWJIOF-SVFFXJIWSA-N |
| Shipping | AMBIENT |
| Short Term Storage | RT |
| Long Term Storage | RT |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at RT. |
| Hazard statements | H351 |
| Precautionary statements | P201 – P308 + P313 |
| GHS Symbol | GHS08 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) E. Adams; Stain Technol. 50, 227 (1975), (2) D.K. Cooper; Histochem. J. 9, 285 (1977), (3) K. Pihlman & E. Linder; Histochem. 79, 157 (1983), (4) T.J. Beveridge; Biotech. Histochem. 76, 111 (2001), (5) T. Frisch, et al.; Eur. Arch. Otorhinolaryngol. 258, 55 (2001), (6) R. Coico, et al.; Curr. Protoc. Microbiol. Appendix 3-3C (2005), (7) C. Gordon, et al.; Int. J. Tuberc. Lung. Dis. 13, 130 (2009), (8) M. Chen, et al.; Bioanalysis 5, 1545 (2013), (9) B. Pathrose, et al.; Spectrochim. Acta A Mol. Biomol. Spectrosc. 128, 522 (2014), (10) B. Pathrose, et al.; J. Fluoresc. 24, 895 (2014) |
| InChi | InChI=1S/C20H19N3.ClH/c1-13-12-16(6-11-19(13)23)20(14-2-7-17(21)8-3-14)15-4-9-18(22)10-5-15;/h2-12,21H,22-23H2,1H3;1H |
| Quantity | 25 g, 100 g, Bulk |
| cdx-UV/Vis | 545 – 555 nm |
