Specifications
- Purity
- ≥95% (HPCE)
- Appearance
- Liquid
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO
- Fluorescence
- λex 482 nm, λem 528 nm in 0.1 M Tris pH 8.0
Documentation
- Safety Data Sheet (SDS)
- CDX C0603 SDS_V1.2.pdf
Available from stock
BCECF-AM solution (5 mM in DMSO), 1 mg in 0.25 ml DMSO
- SKU
- C0603
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- BCECF-AM solution , Spiro(isobenzofuran-1(3H),9'-(9H)xanthene)-2',7'-dipropanoic acid
- 117464-70-7
- CAS-Number
- C42H40O21
- Molecular Formula
- 808.69
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- BLUE ICE
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Hazard statements
- H315, H319, H335
- Precautionary statements
- P261, P305 + P351 + P338
- GHS Symbol
- GHS07
- Signal word
- Warning
- Transportation
- Not dangerous goods
- Description
- Widely-used fluorescent indicator for estimating intracellular pH. This membrane-permeant compound is modified by intracellular esterases to produce BCECF, a polar fluorescein derivative that is retained by cells. Intracellular BCECF is stable and has an efflux half-life of greater than two hours. Intracellular pH measurements are made by ratio imaging, which involves determining the pH-dependent ratio of emission intensity (detected at 535nm) when the dye is excited at 490nm vs. the emission intensity when excited at 440nm. This approach is amenable to either spectrofluorometry or flow cytometry. BCECF-AM ester can also be used to investigate intracellular changes in other ions, including potassium. Spectral properties: ?ex 490nm| ?em 535nm| ?ex=505nm| ?em=520nm in DMSO.
- Smiles
- CC(=O)OCOC(=O)CCC1=CC2=C(OC3=CC(OCOC(C)=O)=C(CCC(=O)OCOC(C)=O)C=C3C22OC(=O)C3=C2C=CC(=C3)C(=O)OCOC(C)=O)C=C1OCOC(C)=O
- InChi Key
- NTECHUXHORNEGZ-UHFFFAOYSA-N
- References
- (1) T.J. Rink, et al. , J. Cell Biol. 95, 189 (1982) , (2) C. Dive, et al. , Mol. Cell Probe 2, 131 (1988) , (3) A.K. Ho, et al. , Am. J. Physiol. 261, C642 (1991) , (4) W. Obexer, et al , Tropical Med. Parasit. 46(1), 45 (1995) , (5) S. Chow, et al. , Cytometry 24, 360 (1996) , (6) H. Urano, et al. , Chem. Lett. 12, 1217 (1997) , (7) L. Balkay, et al. , Cytometry 28, 42 (1997) , (8) P. Ozkan & R. Mutharasan , Biochim. Biophys Acta 1572, 143 (2002) , (9) N. O'Connor & R.B. Silver , Meth. Cell Biol. 81, 415 (2007)
- InChi
- InChI=1S/C42H40O21/c1-22(43)52-17-57-34-15-36-32(13-27(34)7-10-38(48)59-19-54-24(3)45)42(31-9-6-29(12-30(31)41(51)63-42)40(50)61-21-56-26(5)47)33-14-28(8-11-39(49)60-20-55-25(4)46)35(16-37(33)62-36)58-18-53-23(2)44/h6,9,12-16H,7-8,10-11,17-21H2,1-5H3
Widely-used fluorescent indicator for estimating intracellular pH. This membrane-permeant compound is modified by intracellular esterases to produce BCECF, a polar fluorescein derivative that is retained by cells. Intracellular BCECF is stable and has an efflux half-life of greater than two hours. Intracellular pH measurements are made by ratio imaging, which involves determining the pH-dependent ratio of emission intensity (detected at 535nm) when the dye is excited at 490nm vs. the emission intensity when excited at 440nm. This approach is amenable to either spectrofluorometry or flow cytometry. BCECF-AM ester can also be used to investigate intracellular changes in other ions, including potassium. Spectral properties: ?ex 490nm| ?em 535nm| ?ex=505nm| ?em=520nm in DMSO.
Additional information
| Synonyms | BCECF-AM solution, Spiro(isobenzofuran-1(3H),9'-(9H)xanthene)-2',7'-dipropanoic acid |
|---|---|
| Purity | ≥95% (HPCE) |
| Appearance | Liquid |
| CAS-Number | 117464-70-7 |
| Molecular Formula | C42H40O21 |
| Molecular Weight | 808.69 |
| Identity | 1H-NMR |
| Solvents | DMSO |
| Fluorescence | λex 482 nm, λem 528 nm in 0.1 M Tris pH 8.0 |
| Smiles | CC(=O)OCOC(=O)CCC1=CC2=C(OC3=CC(OCOC(C)=O)=C(CCC(=O)OCOC(C)=O)C=C3C22OC(=O)C3=C2C=CC(=C3)C(=O)OCOC(C)=O)C=C1OCOC(C)=O |
| InChi Key | NTECHUXHORNEGZ-UHFFFAOYSA-N |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Hazard statements | H315, H319, H335 |
| Precautionary statements | P261, P305 + P351 + P338 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) T.J. Rink, et al., J. Cell Biol. 95, 189 (1982), (2) C. Dive, et al., Mol. Cell Probe 2, 131 (1988), (3) A.K. Ho, et al., Am. J. Physiol. 261, C642 (1991), (4) W. Obexer, et al, Tropical Med. Parasit. 46(1), 45 (1995), (5) S. Chow, et al., Cytometry 24, 360 (1996), (6) H. Urano, et al., Chem. Lett. 12, 1217 (1997), (7) L. Balkay, et al., Cytometry 28, 42 (1997), (8) P. Ozkan & R. Mutharasan, Biochim. Biophys Acta 1572, 143 (2002), (9) N. O'Connor & R.B. Silver, Meth. Cell Biol. 81, 415 (2007) |
| InChi | InChI=1S/C42H40O21/c1-22(43)52-17-57-34-15-36-32(13-27(34)7-10-38(48)59-19-54-24(3)45)42(31-9-6-29(12-30(31)41(51)63-42)40(50)61-21-56-26(5)47)33-14-28(8-11-39(49)60-20-55-25(4)46)35(16-37(33)62-36)58-18-53-23(2)44/h6,9,12-16H,7-8,10-11,17-21H2,1-5H3 |
| Quantity | 1 mg, Bulk |
