BCECF-AM solution (5 mM in DMSO), 1 mg in 0.25 ml DMSO

SKU: C0603

Categories: Fluorescent Detection, Fluorescent Detection

Synonyms: BCECF-AM solution , Spiro(isobenzofuran-1(3H),9'-(9H)xanthene)-2',7'-dipropanoic acid; 117464-70-7
CAS-Number: C₄₂H₄₀O₂₁
Molecular Formula: 808.69
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥95% (HPCE)
Identity: 1H-NMR
Appearance: Liquid

Properties

Fluorescence: λex 482 nm, λem 528 nm in 0.1 M Tris pH 8.0
Solvents: DMSO

Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.
Long Term Storage: -20°C
Short Term Storage: +4°C
Shipping: BLUE ICE

Transportation: Not dangerous goods
Signal word: Warning
GHS Symbol: GHS07
Precautionary statements: P261, P305 + P351 + P338
Hazard statements: H315, H319, H335

Description: Widely-used fluorescent indicator for estimating intracellular pH. This membrane-permeant compound is modified by intracellular esterases to produce BCECF, a polar fluorescein derivative that is retained by cells. Intracellular BCECF is stable and has an efflux half-life of greater than two hours. Intracellular pH measurements are made by ratio imaging, which involves determining the pH-dependent ratio of emission intensity (detected at 535nm) when the dye is excited at 490nm vs. the emission intensity when excited at 440nm. This approach is amenable to either spectrofluorometry or flow cytometry. BCECF-AM ester can also be used to investigate intracellular changes in other ions, including potassium. Spectral properties: ?ex 490nm| ?em 535nm| ?ex=505nm| ?em=520nm in DMSO.
InChi: InChI=1S/C42H40O21/c1-22(43)52-17-57-34-15-36-32(13-27(34)7-10-38(48)59-19-54-24(3)45)42(31-9-6-29(12-30(31)41(51)63-42)40(50)61-21-56-26(5)47)33-14-28(8-11-39(49)60-20-55-25(4)46)35(16-37(33)62-36)58-18-53-23(2)44/h6,9,12-16H,7-8,10-11,17-21H2,1-5H3
References: (1) T.J. Rink, et al. , J. Cell Biol. 95, 189 (1982) , (2) C. Dive, et al. , Mol. Cell Probe 2, 131 (1988) , (3) A.K. Ho, et al. , Am. J. Physiol. 261, C642 (1991) , (4) W. Obexer, et al , Tropical Med. Parasit. 46(1), 45 (1995) , (5) S. Chow, et al. , Cytometry 24, 360 (1996) , (6) H. Urano, et al. , Chem. Lett. 12, 1217 (1997) , (7) L. Balkay, et al. , Cytometry 28, 42 (1997) , (8) P. Ozkan & R. Mutharasan , Biochim. Biophys Acta 1572, 143 (2002) , (9) N. O'Connor & R.B. Silver , Meth. Cell Biol. 81, 415 (2007)
InChi Key: NTECHUXHORNEGZ-UHFFFAOYSA-N
Smiles: CC(=O)OCOC(=O)CCC1=CC2=C(OC3=CC(OCOC(C)=O)=C(CCC(=O)OCOC(C)=O)C=C3C22OC(=O)C3=C2C=CC(=C3)C(=O)OCOC(C)=O)C=C1OCOC(C)=O

Widely-used fluorescent indicator for estimating intracellular pH. This membrane-permeant compound is modified by intracellular esterases to produce BCECF, a polar fluorescein derivative that is retained by cells. Intracellular BCECF is stable and has an efflux half-life of greater than two hours. Intracellular pH measurements are made by ratio imaging, which involves determining the pH-dependent ratio of emission intensity (detected at 535nm) when the dye is excited at 490nm vs. the emission intensity when excited at 440nm. This approach is amenable to either spectrofluorometry or flow cytometry. BCECF-AM ester can also be used to investigate intracellular changes in other ions, including potassium. Spectral properties: ?ex 490nm| ?em 535nm| ?ex=505nm| ?em=520nm in DMSO.

SKU: C0603 Categories: Fluorescent Detection, Fluorescent Detection

Additional information

Additional information

Synonyms	BCECF-AM solution, Spiro(isobenzofuran-1(3H),9'-(9H)xanthene)-2',7'-dipropanoic acid
Purity	≥95% (HPCE)
Appearance	Liquid
CAS-Number	117464-70-7
Molecular Formula	C42H40O21
Molecular Weight	808.69
Identity	1H-NMR
Solvents	DMSO
Fluorescence	λex 482 nm, λem 528 nm in 0.1 M Tris pH 8.0
Smiles	CC(=O)OCOC(=O)CCC1=CC2=C(OC3=CC(OCOC(C)=O)=C(CCC(=O)OCOC(C)=O)C=C3C22OC(=O)C3=C2C=CC(=C3)C(=O)OCOC(C)=O)C=C1OCOC(C)=O
InChi Key	NTECHUXHORNEGZ-UHFFFAOYSA-N
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Hazard statements	H315, H319, H335
Precautionary statements	P261, P305 + P351 + P338
GHS Symbol	GHS07
Signal word	Warning
Transportation	Not dangerous goods
References	(1) T.J. Rink, et al., J. Cell Biol. 95, 189 (1982), (2) C. Dive, et al., Mol. Cell Probe 2, 131 (1988), (3) A.K. Ho, et al., Am. J. Physiol. 261, C642 (1991), (4) W. Obexer, et al, Tropical Med. Parasit. 46(1), 45 (1995), (5) S. Chow, et al., Cytometry 24, 360 (1996), (6) H. Urano, et al., Chem. Lett. 12, 1217 (1997), (7) L. Balkay, et al., Cytometry 28, 42 (1997), (8) P. Ozkan & R. Mutharasan, Biochim. Biophys Acta 1572, 143 (2002), (9) N. O'Connor & R.B. Silver, Meth. Cell Biol. 81, 415 (2007)
InChi	InChI=1S/C42H40O21/c1-22(43)52-17-57-34-15-36-32(13-27(34)7-10-38(48)59-19-54-24(3)45)42(31-9-6-29(12-30(31)41(51)63-42)40(50)61-21-56-26(5)47)33-14-28(8-11-39(49)60-20-55-25(4)46)35(16-37(33)62-36)58-18-53-23(2)44/h6,9,12-16H,7-8,10-11,17-21H2,1-5H3
Quantity	1 mg, Bulk