Specifications
- Purity
- ≥98% (HPLC)
- Appearance
- White to off-white powder
- Identity
- 1H-NMR
Properties
- Solvents
- DMF, DMSO
Available from stock
(+)-Biotin N-hydroxysuccinimide ester
- SKU
- B0145
Categories:
- Synonyms
- (+)-Biotin N-succinimidyl ester , d-Biotin NHS ester , BNHS , Biotinyl-N-hydroxy-succinimide , N-Hydroxysuccinimidobiotin , N-Succinimidyl D-biotinate , NHS-Biotin , Biotin-Osu , NSC 345668
- 35013-72-0
- CAS-Number
- C14H19N3O5S
- Molecular Formula
- 341.38
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Transportation
- Not dangerous goods
- Description
- Biotin succinimidyl ester is the most popular amine-reactive biotinylating agent for modifying proteins, peptides, antibodies, oligonucleotides, microbeads and other biological molecules. It forms irreversible amide bonds. Typically coupled to primary amine in the pH range 6.5-8.5. This cell-permable activated biotin derivative can be readily coupled to primary or secondary amines of analyte compounds or proteins under mild conditions. The resulting biotinylated derivatives have been widely used in streptavidin or avidin systems and labeling methods, with many applications in immunology and histochemistry. It is used to selectively label Escherichia coli cell envelope proteins in vivo. It preferentially labels outer membrane, periplasmic, and inner membrane proteins as well as a specific inner membrane marker protein (Tet-LacZ).
- Smiles
- C1CC(=O)N(C1=O)OC(=O)CCCCC2C3C(CS2)NC(=O)N3
- InChi Key
- YMXHPSHLTSZXKH-RVBZMBCESA-N
- References
- (1) D.M. Boorsma, et al., J. Microsc. 143, 197 (1986) , (2) P. Kongtawelert & P. Ghosh, Clin. Chem. Acta 195, 17 (1990) , (3) G. Paganelli, et al., Int. J. Cancer 45, 1184 (1990) , (4) C. Wagener, et al., Meth. Enzymol. 184, 518 (1990) , (5) B. Baumeister, et al., Int. J. Pep. Res. Therap. 11, 139 (2005) , (6) G.T. Hermanson, Bioconjugate Techn. 2nd p506-545 (2008)
Biotin succinimidyl ester is the most popular amine-reactive biotinylating agent for modifying proteins, peptides, antibodies, oligonucleotides, microbeads and other biological molecules. It forms irreversible amide bonds. Typically coupled to primary amine in the pH range 6.5-8.5. This cell-permable activated biotin derivative can be readily coupled to primary or secondary amines of analyte compounds or proteins under mild conditions. The resulting biotinylated derivatives have been widely used in streptavidin or avidin systems and labeling methods, with many applications in immunology and histochemistry. It is used to selectively label Escherichia coli cell envelope proteins in vivo. It preferentially labels outer membrane, periplasmic, and inner membrane proteins as well as a specific inner membrane marker protein (Tet-LacZ).
Additional information
| Synonyms | (+)-Biotin N-succinimidyl ester, d-Biotin NHS ester, BNHS, Biotinyl-N-hydroxy-succinimide, N-Hydroxysuccinimidobiotin, N-Succinimidyl D-biotinate, NHS-Biotin, Biotin-Osu, NSC 345668 |
|---|---|
| Purity | ≥98% (HPLC) |
| Appearance | White to off-white powder |
| CAS-Number | 35013-72-0 |
| Molecular Formula | C14H19N3O5S |
| Molecular Weight | 341.38 |
| Identity | 1H-NMR |
| Solvents | DMF, DMSO |
| Smiles | C1CC(=O)N(C1=O)OC(=O)CCCCC2C3C(CS2)NC(=O)N3 |
| InChi Key | YMXHPSHLTSZXKH-RVBZMBCESA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Transportation | Not dangerous goods |
| References | (1) D.M. Boorsma, et al., J. Microsc. 143, 197 (1986), (2) P. Kongtawelert & P. Ghosh, Clin. Chem. Acta 195, 17 (1990), (3) G. Paganelli, et al., Int. J. Cancer 45, 1184 (1990), (4) C. Wagener, et al., Meth. Enzymol. 184, 518 (1990), (5) B. Baumeister, et al., Int. J. Pep. Res. Therap. 11, 139 (2005), (6) G.T. Hermanson, Bioconjugate Techn. 2nd p506-545 (2008) |
| Quantity | 100 mg, 250 mg, Bulk |
