Specifications
- Purity
- ≥97% (NMR)
- Appearance
- White to off-white powder
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO (20 mg/ml), DMF(20 mg/ml), methanol
Available from stock
Biotin-X-NHS
- SKU
- B0139
Category: Building Blocks, Intermediates, Reagents
- Synonyms
- NHS-LC-Biotin , Biotinamidohexanoic acid N-hydroxysuccinimide ester , (+)-Biotinamidocaproate N-hydroxysuccinimidyl ester , N-(+)-Biotinyl-6-aminocaproic acid N-succinimidyl ester , N-(+)-Biotinyl-6-aminohexanoic acid NHS ester , Enzotin
- 72040-63-2
- CAS-Number
- C20H30N4O6S
- Molecular Formula
- 454.54
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- -20°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Transportation
- Not dangerous goods
- Description
- Biotin-X-NHS is an amino-reactive biotinylation reagent with a long spacer arm. The 6-aminocaproic acid spacer arm provides distance between the biotin and the target ligand which helps alleviate steric hindrance. Biotin-X-NHS is used to biotinylate antibodies, proteins, peptides, or amino acids by reacting with primary amines as found in the N-terminus and Lysine side chains. The Biotin-X-NHS molecule is cell membrane permeable and can be used to label intracellular proteins. Optimal reaction conditions are at a pH of 7-9. Avoid amine-containing buffers, which may compete in the acylation reaction.
- Smiles
- O=C(CCCCC1SCC2NC(=O)NC12)NCCCCCC(=O)ON1C(=O)CCC1=O
- InChi Key
- UVGHPGOONBRLCX-NJSLBKSFSA-N
- References
- (1) G.T. Hermanson, Bioconjugate Techn. 2nd p506-545 (2008)
Biotin-X-NHS is an amino-reactive biotinylation reagent with a long spacer arm. The 6-aminocaproic acid spacer arm provides distance between the biotin and the target ligand which helps alleviate steric hindrance. Biotin-X-NHS is used to biotinylate antibodies, proteins, peptides, or amino acids by reacting with primary amines as found in the N-terminus and Lysine side chains. The Biotin-X-NHS molecule is cell membrane permeable and can be used to label intracellular proteins. Optimal reaction conditions are at a pH of 7-9. Avoid amine-containing buffers, which may compete in the acylation reaction.
Additional information
| Synonyms | NHS-LC-Biotin, Biotinamidohexanoic acid N-hydroxysuccinimide ester, (+)-Biotinamidocaproate N-hydroxysuccinimidyl ester, N-(+)-Biotinyl-6-aminocaproic acid N-succinimidyl ester, N-(+)-Biotinyl-6-aminohexanoic acid NHS ester, Enzotin |
|---|---|
| Purity | ≥97% (NMR) |
| Appearance | White to off-white powder |
| CAS-Number | 72040-63-2 |
| Molecular Formula | C20H30N4O6S |
| Molecular Weight | 454.54 |
| Identity | 1H-NMR |
| Solvents | DMSO (20 mg/ml), DMF(20 mg/ml), methanol |
| Smiles | O=C(CCCCC1SCC2NC(=O)NC12)NCCCCCC(=O)ON1C(=O)CCC1=O |
| InChi Key | UVGHPGOONBRLCX-NJSLBKSFSA-N |
| Shipping | AMBIENT |
| Short Term Storage | -20°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Transportation | Not dangerous goods |
| References | (1) G.T. Hermanson, Bioconjugate Techn. 2nd p506-545 (2008) |
| Quantity | 25 mg, 100 mg, Bulk |
