Specifications
- Purity
- ≥94% (Assay)
- Appearance
- Solid
- Identity
- 1H-NMR
Properties
- Solvents
- Soluble in ethanol (20 mg/ml), methanol or DMSO. Insoluble in water.
- Melting Point
- 240 °C (dec.) (lit.)
Available from stock
Bromocresol Purple
- SKU
- B0055
Category: Non Fluorescent Detection & Chromogene Detection
- Synonyms
- NSC 374134, BCP, 5,5'-Dibromo-o-cresolsulfonphthalein,
- 115-40-2
- CAS-Number
- C21H16Br2O5S
- Molecular Formula
- 540.22
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- RT
- Long Term Storage
- RT
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at RT.
- Transportation
- Not dangerous goods
- Description
- Bromocresol Purple (BCP) is a pH-sensitive dye widely used as a colorimetric pH indicator and analytical reagent. It exhibits a distinct color change from yellow at pH <5.2 to purple at pH >6.8, making it valuable in titrations, buffer preparation and biochemical assays. In clinical and laboratory settings, it is commonly used in protein and albumin assays due to its ability to form colored complexes under acidic conditions. In yeast it helps in dead cell count for cells with plasma membrane damage. Cells with proper cell membrane are not stained, while damaged cells are seen as blue-grey ghosts.
- Smiles
- BrC1=C(O)C(C)=CC(C(OS2(=O)=O)(C3=CC(C)=C(O)C(Br)=C3)C4=C2C=CC=C4)=C1
- InChi Key
- ABIUHPWEYMSGSR-UHFFFAOYSA-N
- References
- (1) R.M. Tel, et al., J. Clin. Chem. Clin. Biochem. 17, 627 (1979) , (2) P.G. Hill, Ann. Clin. Biochem. 22, 565 (1985) , (3) H. Kurzweilova & K. Sigler, Yeast 9, 1207 (1993) , (4) B.T. Doumas & T. Peters, Clin. Chim. Acta 258, 3 (1997) , (5) S. Ito & D. Yamamoto, Clin. Chim. Acta 411, 294 (2010) , (6) A.L. Silva, et al., Bio. Protoc. 8, e2796 (2018) , (7) S. Yoshihiro, et al., J. Clin. Biochem. Nutr. 67, 257 (2020) , (8) H. Khanjanzadeh & B.D. Park, Carbohydr. Polym. 273, 118550 (2021)
- InChi
- InChI=1S/C21H16Br2O5S/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21/h3-10,24-25H,1-2H3
Bromocresol Purple (BCP) is a pH-sensitive dye widely used as a colorimetric pH indicator and analytical reagent. It exhibits a distinct color change from yellow at pH <5.2 to purple at pH >6.8, making it valuable in titrations, buffer preparation and biochemical assays. In clinical and laboratory settings, it is commonly used in protein and albumin assays due to its ability to form colored complexes under acidic conditions. In yeast it helps in dead cell count for cells with plasma membrane damage. Cells with proper cell membrane are not stained, while damaged cells are seen as blue-grey ghosts.
Additional information
| Synonyms | NSC 374134, BCP, 5,5'-Dibromo-o-cresolsulfonphthalein, |
|---|---|
| Purity | ≥94% (Assay) |
| Appearance | Solid |
| CAS-Number | 115-40-2 |
| Molecular Formula | C21H16Br2O5S |
| Molecular Weight | 540.22 |
| Identity | 1H-NMR |
| Solvents | Soluble in ethanol (20 mg/ml), methanol or DMSO. Insoluble in water. |
| Melting Point | 240 °C (dec.) (lit.) |
| Smiles | BrC1=C(O)C(C)=CC(C(OS2(=O)=O)(C3=CC(C)=C(O)C(Br)=C3)C4=C2C=CC=C4)=C1 |
| InChi Key | ABIUHPWEYMSGSR-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | RT |
| Long Term Storage | RT |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at RT. |
| Transportation | Not dangerous goods |
| References | (1) R.M. Tel, et al., J. Clin. Chem. Clin. Biochem. 17, 627 (1979), (2) P.G. Hill, Ann. Clin. Biochem. 22, 565 (1985), (3) H. Kurzweilova & K. Sigler, Yeast 9, 1207 (1993), (4) B.T. Doumas & T. Peters, Clin. Chim. Acta 258, 3 (1997), (5) S. Ito & D. Yamamoto, Clin. Chim. Acta 411, 294 (2010), (6) A.L. Silva, et al., Bio. Protoc. 8, e2796 (2018), (7) S. Yoshihiro, et al., J. Clin. Biochem. Nutr. 67, 257 (2020), (8) H. Khanjanzadeh & B.D. Park, Carbohydr. Polym. 273, 118550 (2021) |
| InChi | InChI=1S/C21H16Br2O5S/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21/h3-10,24-25H,1-2H3 |
| Quantity | 10 g, 50 g, Bulk |
| cdx-Absorbtion max. | 429-433 nm (pH 5.2) |
