Specifications
- Purity
- ≥95% (Dye content)
- Appearance
- Violet powder
- Loss on Drying
- ≥3% at 110 degrees C (1hr)
Properties
- Solvents
- Soluble in DMSO. Sparingly soluble in water.
- Melting Point
- 200-202 °C (lit.)
Available from stock
Bromothymol Blue
- SKU
- B0049
Category: Non Fluorescent Detection & Chromogene Detection
- Synonyms
- 3,3?-Dibromothymolsulfophthalein, Bromothymolsulfonephthalein, NSC 7819
- 76-59-5
- CAS-Number
- C27H28Br2O5S
- Molecular Formula
- 624.38
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- RT
- Long Term Storage
- RT
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at RT.
- Transportation
- Not dangerous goods
- Description
- Bromothymol Blue (BTB) is a sulfonephthalein-based pH indicator dye with a sharp and reversible color transition near neutrality: yellow in acidic conditions, blue in alkaline media, with a clear transition interval around pH 6.0–7.6. Bromothymol Blue is widely applied in analytical chemistry and education as a reliable acid–base indicator for titrations and demonstrations of pH changes. In microbiology and biotechnology it is incorporated into culture media to reveal microbial metabolism, acid formation, or carbon dioxide release, while in environmental monitoring it serves as a practical tool for assessing water quality and maintaining stable pH in aquaculture systems. The compound also finds use in physiological and medical research where it assists in the study of respiration, photosynthesis, and other pH-sensitive biochemical processes. Beyond the laboratory, Bromothymol Blue is a common component of indicator solutions and pH testing kits.
- Smiles
- O=S1(OC(C2=C(C)C(Br)=C(O)C(C(C)C)=C2)(C3=C(C)C(Br)=C(O)C(C(C)C)=C3)C4=C1C=CC=C4)=O
- InChi Key
- NUHCTOLBWMJMLX-UHFFFAOYSA-N
- References
- (1) J.B. Jackson & A.R. Crofts, Eur. J. Biochem. 10, 226 (1969), (2) Z. Aleksandrowicz & J. Swierczynski, FEBS Lett, 20, 364 (1972), (3) J.B. Puschett, et al., Talanta 38, 335 (1991), (4) R.W. Sabnis, Handbook of Acid-Base Indicators (2007), (6) R.W. Sabnis, Handbook of biological dyes and stains (2010)
- InChi
- InChI=1S/C27H28Br2O5S/c1-13(2)17-11-20(15(5)23(28)25(17)30)27(19-9-7-8-10-22(19)35(32,33)34-27)21-12-18(14(3)4)26(31)24(29)16(21)6/h7-14,30-31H,1-6H3
Bromothymol Blue (BTB) is a sulfonephthalein-based pH indicator dye with a sharp and reversible color transition near neutrality: yellow in acidic conditions, blue in alkaline media, with a clear transition interval around pH 6.0–7.6. Bromothymol Blue is widely applied in analytical chemistry and education as a reliable acid–base indicator for titrations and demonstrations of pH changes. In microbiology and biotechnology it is incorporated into culture media to reveal microbial metabolism, acid formation, or carbon dioxide release, while in environmental monitoring it serves as a practical tool for assessing water quality and maintaining stable pH in aquaculture systems. The compound also finds use in physiological and medical research where it assists in the study of respiration, photosynthesis, and other pH-sensitive biochemical processes. Beyond the laboratory, Bromothymol Blue is a common component of indicator solutions and pH testing kits.
Additional information
| Synonyms | 3,3?-Dibromothymolsulfophthalein, Bromothymolsulfonephthalein, NSC 7819 |
|---|---|
| Purity | ≥95% (Dye content) |
| Appearance | Violet powder |
| CAS-Number | 76-59-5 |
| Molecular Formula | C27H28Br2O5S |
| Molecular Weight | 624.38 |
| Solvents | Soluble in DMSO. Sparingly soluble in water. |
| Melting Point | 200-202 °C (lit.) |
| Smiles | O=S1(OC(C2=C(C)C(Br)=C(O)C(C(C)C)=C2)(C3=C(C)C(Br)=C(O)C(C(C)C)=C3)C4=C1C=CC=C4)=O |
| InChi Key | NUHCTOLBWMJMLX-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | RT |
| Long Term Storage | RT |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at RT. |
| Transportation | Not dangerous goods |
| References | (1) J.B. Jackson & A.R. Crofts, Eur. J. Biochem. 10, 226 (1969), (2) Z. Aleksandrowicz & J. Swierczynski, FEBS Lett, 20, 364 (1972), (3) J.B. Puschett, et al., Talanta 38, 335 (1991), (4) R.W. Sabnis, Handbook of Acid-Base Indicators (2007), (6) R.W. Sabnis, Handbook of biological dyes and stains (2010) |
| InChi | InChI=1S/C27H28Br2O5S/c1-13(2)17-11-20(15(5)23(28)25(17)30)27(19-9-7-8-10-22(19)35(32,33)34-27)21-12-18(14(3)4)26(31)24(29)16(21)6/h7-14,30-31H,1-6H3 |
| Quantity | 25 g, 100 g, Bulk |
| cdx-Absorbtion max. | 615 – 618 nm (pH 7.6) |
| cdx-Loss on Drying | ≥3% at 110 degrees C (1hr) |
