Specifications
- Purity
- ≥95% (Dye content)
- Appearance
- Faint beige to orange to red powder
Properties
- Solvents
- Soluble in DMF (30mg/ml), DMSO (30mg/ml) or ethanol (10mg/ml). Slightly soluble in aqueous buffer (1mg/ml).
- Melting Point
- 273 °C (lit.)
Available from stock
Bromphenol blue
- SKU
- B0026
Category: Non Fluorescent Detection & Chromogene Detection
- Synonyms
- Bromophenol blue, 3',3'',5',5''-Tetrabromophenolsulfonephthalein, NSC 7818, BPB 670, Albutest
- 115-39-9
- CAS-Number
- C19H10Br4O5S
- Molecular Formula
- 669.96
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- RT
- Long Term Storage
- RT
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at RT.
- Transportation
- Not dangerous goods
- Description
- Bromophenol blue is a widely used pH indicator dye and tracking dye for biochemical and analytical applications. It exhibits a distinct color change over its transition range and is commonly employed in acid-base titrations, with a transition range of pH 3 (yellow) to 4.6 (purple), electrophoresis sample loading buffers, and laboratory staining protocols. Bromphenol Blue is used as a tracking dye in DNA, RNA (agarose), and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels. It has also been known for its use as a vital stain to probe the blood-brain barrier. Spectral Data: ?max=610 nm. Bromophenol blue is an essential reagent for research laboratories, offering reliable performance in molecular biology, biochemistry, and analytical chemistry.
- Smiles
- O=S1(=O)OC(C2=CC(Br)=C(O)C(Br)=C2)(C3=CC(Br)=C(O)C(Br)=C3)C=4C=CC=CC41
- InChi Key
- UDSAIICHUKSCKT-UHFFFAOYSA-N
- References
- (1) P. Harris & D.J. Mazia, Biophys. Biochem. Cytol. 5, 343 (1959) , (2) D.W. Menzies, Stain Technol. 36, 285 (1961) , (3) G.D. Burford & B.T. Pickering, Biochem. J. 128, 941 (1972) , (4) R. Flores, Anal. Biochem. 88, 605 (1978) , (5) S. Dhananasekaran, et al., J. Adv. Res. 7, 113 (2016) , (6) A.Z. Dangui, et al., Spectrochim. Acta A Mol. Biomol. Spectrosc. 203, 333 (2018) , (7) C. Burel, et al., Lett. Appl. Microbiol. 72, 358 (2021)
- InChi
- InChI=1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H
Bromophenol blue is a widely used pH indicator dye and tracking dye for biochemical and analytical applications. It exhibits a distinct color change over its transition range and is commonly employed in acid-base titrations, with a transition range of pH 3 (yellow) to 4.6 (purple), electrophoresis sample loading buffers, and laboratory staining protocols. Bromphenol Blue is used as a tracking dye in DNA, RNA (agarose), and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels. It has also been known for its use as a vital stain to probe the blood-brain barrier. Spectral Data: ?max=610 nm. Bromophenol blue is an essential reagent for research laboratories, offering reliable performance in molecular biology, biochemistry, and analytical chemistry.
Additional information
| Synonyms | Bromophenol blue, 3',3'',5',5''-Tetrabromophenolsulfonephthalein, NSC 7818, BPB 670, Albutest |
|---|---|
| Purity | ≥95% (Dye content) |
| Appearance | Faint beige to orange to red powder |
| CAS-Number | 115-39-9 |
| Molecular Formula | C19H10Br4O5S |
| Molecular Weight | 669.96 |
| Solvents | Soluble in DMF (30mg/ml), DMSO (30mg/ml) or ethanol (10mg/ml). Slightly soluble in aqueous buffer (1mg/ml). |
| Melting Point | 273 °C (lit.) |
| Smiles | O=S1(=O)OC(C2=CC(Br)=C(O)C(Br)=C2)(C3=CC(Br)=C(O)C(Br)=C3)C=4C=CC=CC41 |
| InChi Key | UDSAIICHUKSCKT-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | RT |
| Long Term Storage | RT |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at RT. |
| Transportation | Not dangerous goods |
| References | (1) P. Harris & D.J. Mazia, Biophys. Biochem. Cytol. 5, 343 (1959), (2) D.W. Menzies, Stain Technol. 36, 285 (1961), (3) G.D. Burford & B.T. Pickering, Biochem. J. 128, 941 (1972), (4) R. Flores, Anal. Biochem. 88, 605 (1978), (5) S. Dhananasekaran, et al., J. Adv. Res. 7, 113 (2016), (6) A.Z. Dangui, et al., Spectrochim. Acta A Mol. Biomol. Spectrosc. 203, 333 (2018), (7) C. Burel, et al., Lett. Appl. Microbiol. 72, 358 (2021) |
| InChi | InChI=1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H |
| Quantity | 25 g, Bulk |
