CPM

Specifications

Purity

≥95% (HPLC)

Identity

1H-NMR

Appearance

Yellow solid

Properties

Fluorescence

λex 391 nm, λem 472 nm in methanol/2-mercaptoethanol

Solvents

DMSO, DMF, acetonitrile, chloroform

Short Term Storage

+4°C

Use / Stability

Stable for at least 2 years after receipt when stored at -20°C.

Handling Advice

Protect from light and moisture.

Long Term Storage

-20°C

Shipping

AMBIENT

Transportation

Not dangerous goods

Description

Widely used blue fluorescent thiol-reactive dye (Ex/Em: 384/470nm). This maleimide derivative of coumarin is essentially non-fluorescent until it reacts with thiols, making it possible to quantify thiols without a separation step. Good energy acceptor from tryptophan and a good energy donor to fluorescein. Used to monitor release of thiols and to distinguish proliferating cancer cells by nucleolar protein staining.

InChi

InChI=1S/C24H22N2O4/c1-4-25(5-2)18-10-11-19-15(3)23(24(29)30-20(19)14-18)16-6-8-17(9-7-16)26-21(27)12-13-22(26)28/h6-14H,4-5H2,1-3H3

References

(1) C.C. Chung: Assay and Drug Devel. Techn. 6(3), 361 (2008) , (2) O. Khersonsky et al.: ChemBioChem 7(1), 49 (2006) , (3) J.E.T. Corrie et al.: J. Chem. Soc. Trans. Perkin Trans. 1, 2975 (1994)

InChi Key

YGIABALXNBVHBX-UHFFFAOYSA-N

Smiles

CCN(CC)C1=CC2=C(C=C1)C(C)=C(C(=O)O2)C1=CC=C(C=C1)N1C(=O)C=CC1=O