Specifications
- Purity
- ≥95% (HPLC)
- Identity
- 1H-NMR
- Appearance
- Yellow solid
Properties
- Fluorescence
- λex 391 nm, λem 472 nm in methanol/2-mercaptoethanol
- Solvents
- DMSO, DMF, acetonitrile, chloroform
Available from stock
CPM
- SKU
- D0042
Category: Fluorescent Detection
- Synonyms
- 7-Diethylamino-3-(4-maleimidylphenyl)-4-methylcoumarin
- 76877-33-3
- CAS-Number
- C24H22N2O4
- Molecular Formula
- 402.44
- Molecular Weight
- Short Term Storage
- +4°C
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Handling Advice
- Protect from light and moisture.
- Long Term Storage
- -20°C
- Shipping
- AMBIENT
- Transportation
- Not dangerous goods
- Description
- Widely used blue fluorescent thiol-reactive dye (Ex/Em: 384/470nm). This maleimide derivative of coumarin is essentially non-fluorescent until it reacts with thiols, making it possible to quantify thiols without a separation step. Good energy acceptor from tryptophan and a good energy donor to fluorescein. Used to monitor release of thiols and to distinguish proliferating cancer cells by nucleolar protein staining.
- InChi
- InChI=1S/C24H22N2O4/c1-4-25(5-2)18-10-11-19-15(3)23(24(29)30-20(19)14-18)16-6-8-17(9-7-16)26-21(27)12-13-22(26)28/h6-14H,4-5H2,1-3H3
- References
- (1) C.C. Chung: Assay and Drug Devel. Techn. 6(3), 361 (2008) , (2) O. Khersonsky et al.: ChemBioChem 7(1), 49 (2006) , (3) J.E.T. Corrie et al.: J. Chem. Soc. Trans. Perkin Trans. 1, 2975 (1994)
- InChi Key
- YGIABALXNBVHBX-UHFFFAOYSA-N
- Smiles
- CCN(CC)C1=CC2=C(C=C1)C(C)=C(C(=O)O2)C1=CC=C(C=C1)N1C(=O)C=CC1=O
Widely used blue fluorescent thiol-reactive dye (Ex/Em: 384/470nm). This maleimide derivative of coumarin is essentially non-fluorescent until it reacts with thiols, making it possible to quantify thiols without a separation step. Good energy acceptor from tryptophan and a good energy donor to fluorescein. Used to monitor release of thiols and to distinguish proliferating cancer cells by nucleolar protein staining.
Additional information
| Synonyms | 7-Diethylamino-3-(4-maleimidylphenyl)-4-methylcoumarin |
|---|---|
| Purity | ≥95% (HPLC) |
| Appearance | Yellow solid |
| CAS-Number | 76877-33-3 |
| Molecular Formula | C24H22N2O4 |
| Molecular Weight | 402.44 |
| Identity | 1H-NMR |
| Solvents | DMSO, DMF, acetonitrile, chloroform |
| Fluorescence | λex 391 nm, λem 472 nm in methanol/2-mercaptoethanol |
| Smiles | CCN(CC)C1=CC2=C(C=C1)C(C)=C(C(=O)O2)C1=CC=C(C=C1)N1C(=O)C=CC1=O |
| InChi Key | YGIABALXNBVHBX-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Transportation | Not dangerous goods |
| References | (1) C.C. Chung: Assay and Drug Devel. Techn. 6(3), 361 (2008), (2) O. Khersonsky et al.: ChemBioChem 7(1), 49 (2006), (3) J.E.T. Corrie et al.: J. Chem. Soc. Trans. Perkin Trans. 1, 2975 (1994) |
| InChi | InChI=1S/C24H22N2O4/c1-4-25(5-2)18-10-11-19-15(3)23(24(29)30-20(19)14-18)16-6-8-17(9-7-16)26-21(27)12-13-22(26)28/h6-14H,4-5H2,1-3H3 |
| Quantity | 50 mg, 250 mg, Bulk |
