- Synonyms: 7-Diethylamino-3-(4-maleimidylphenyl)-4-methylcoumarin
- Purity: ≥95% (HPLC)
- Appearance: Yellow solid.
- CAS-Number: 76877-33-3
- Molecular Formula: C24H22N2O4
- Molecular Weight: 402.44
- Identity: 1H-NMR
- Solvents: DMSO, DMF, acetonitrile or chloroform
- Fluorescence: λex 391 nm; λem 472 nm in methanol/2-mercaptoethanol
- Smiles: CCN(CC)C1=CC2=C(C=C1)C(C)=C(C(=O)O2)C1=CC=C(C=C1)N1C(=O)C=CC1=O
- InChi: InChI=1S/C24H22N2O4/c1-4-25(5-2)18-10-11-19-15(3)23(24(29)30-20(19)14-18)16-6-8-17(9-7-16)26-21(27)12-13-22(26)28/h6-14H,4-5H2,1-3H3
- InChi Key: YGIABALXNBVHBX-UHFFFAOYSA-N
Available from stock
CPM
SKU: D0042
Category: Fluorescent Detection
- Shipping: AMBIENT
- Short Term Storage: +4°C
- Long Term Storage: -20°C
- Handling Advice: Protect from light and moisture.
- Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.
- Description: Widely used blue fluorescent thiol-reactive dye (Ex/Em: 384/470nm). This maleimide derivative of coumarin is essentially non-fluorescent until it reacts with thiols, making it possible to quantify thiols without a separation step. Good energy acceptor from tryptophan and a good energy donor to fluorescein. Used to monitor release of thiols and to distinguish proliferating cancer cells by nucleolar protein staining.
- References: (1) C.C. Chung; Assay and Drug Devel. Techn. 6(3), 361 (2008), (2) O. Khersonsky et al.; ChemBioChem 7(1), 49 (2006), (3) J.E.T. Corrie et al.; J. Chem. Soc. Trans. Perkin Trans. 1, 2975 (1994)
Widely used blue fluorescent thiol-reactive dye (Ex/Em: 384/470nm). This maleimide derivative of coumarin is essentially non-fluorescent until it reacts with thiols, making it possible to quantify thiols without a separation step. Good energy acceptor from tryptophan and a good energy donor to fluorescein. Used to monitor release of thiols and to distinguish proliferating cancer cells by nucleolar protein staining.
Additional information
| Identity | 1H-NMR |
|---|---|
| Handling Advice | Protect from light and moisture. |
| Availability | 1 |
| CAS-Number | 76877-33-3 |
| Long Term Storage | -20°C |
| InChi Key | YGIABALXNBVHBX-UHFFFAOYSA-N |
| Quantity | 250 mg, 50 mg, Bulk |
| Appearance | Yellow solid. |
| InChi | InChI=1S/C24H22N2O4/c1-4-25(5-2)18-10-11-19-15(3)23(24(29)30-20(19)14-18)16-6-8-17(9-7-16)26-21(27)12-13-22(26)28/h6-14H,4-5H2,1-3H3 |
| Fluorescence | λex 391 nm; λem 472 nm in methanol/2-mercaptoethanol |
| Molecular Formula | |
| Short Term Storage | +4°C |
| Purity | |
| Pricetable | |
| References | (1) C.C. Chung; Assay and Drug Devel. Techn. 6(3), 361 (2008), (2) O. Khersonsky et al.; ChemBioChem 7(1), 49 (2006), (3) J.E.T. Corrie et al.; J. Chem. Soc. Trans. Perkin Trans. 1, 2975 (1994) |
| Smiles | CCN(CC)C1=CC2=C(C=C1)C(C)=C(C(=O)O2)C1=CC=C(C=C1)N1C(=O)C=CC1=O |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Molecular Weight | |
| Solvents | DMSO, DMF, acetonitrile or chloroform |
| Synonyms | 7-Diethylamino-3-(4-maleimidylphenyl)-4-methylcoumarin |
| Shipping | AMBIENT |
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