Available from stock

CPM

SKU: D0042

Category: Fluorescent Detection

Synonyms: 7-Diethylamino-3-(4-maleimidylphenyl)-4-methylcoumarin; 76877-33-3
CAS-Number: C₂₄H₂₂N₂O₄
Molecular Formula: 402.44
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥95% (HPLC)
Appearance: Yellow solid
Identity: 1H-NMR

Properties

Solvents: DMSO, DMF, acetonitrile, chloroform
Fluorescence: λex 391 nm, λem 472 nm in methanol/2-mercaptoethanol

Documentation

Safety Data Sheet (SDS): CDX D0042 MSDS.pdf

Shipping: AMBIENT
Short Term Storage: +4°C
Long Term Storage: -20°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.

Transportation: Not dangerous goods

Description: Widely used blue fluorescent thiol-reactive dye (Ex/Em: 384/470nm). This maleimide derivative of coumarin is essentially non-fluorescent until it reacts with thiols, making it possible to quantify thiols without a separation step. Good energy acceptor from tryptophan and a good energy donor to fluorescein. Used to monitor release of thiols and to distinguish proliferating cancer cells by nucleolar protein staining.
Smiles: CCN(CC)C1=CC2=C(C=C1)C(C)=C(C(=O)O2)C1=CC=C(C=C1)N1C(=O)C=CC1=O
InChi Key: YGIABALXNBVHBX-UHFFFAOYSA-N
References: (1) C.C. Chung: Assay and Drug Devel. Techn. 6(3), 361 (2008) , (2) O. Khersonsky et al.: ChemBioChem 7(1), 49 (2006) , (3) J.E.T. Corrie et al.: J. Chem. Soc. Trans. Perkin Trans. 1, 2975 (1994)
InChi: InChI=1S/C24H22N2O4/c1-4-25(5-2)18-10-11-19-15(3)23(24(29)30-20(19)14-18)16-6-8-17(9-7-16)26-21(27)12-13-22(26)28/h6-14H,4-5H2,1-3H3

Widely used blue fluorescent thiol-reactive dye (Ex/Em: 384/470nm). This maleimide derivative of coumarin is essentially non-fluorescent until it reacts with thiols, making it possible to quantify thiols without a separation step. Good energy acceptor from tryptophan and a good energy donor to fluorescein. Used to monitor release of thiols and to distinguish proliferating cancer cells by nucleolar protein staining.

SKU: D0042 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	7-Diethylamino-3-(4-maleimidylphenyl)-4-methylcoumarin
Purity	≥95% (HPLC)
Appearance	Yellow solid
CAS-Number	76877-33-3
Molecular Formula	C24H22N2O4
Molecular Weight	402.44
Identity	1H-NMR
Solvents	DMSO, DMF, acetonitrile, chloroform
Fluorescence	λex 391 nm, λem 472 nm in methanol/2-mercaptoethanol
Smiles	CCN(CC)C1=CC2=C(C=C1)C(C)=C(C(=O)O2)C1=CC=C(C=C1)N1C(=O)C=CC1=O
InChi Key	YGIABALXNBVHBX-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Transportation	Not dangerous goods
References	(1) C.C. Chung: Assay and Drug Devel. Techn. 6(3), 361 (2008), (2) O. Khersonsky et al.: ChemBioChem 7(1), 49 (2006), (3) J.E.T. Corrie et al.: J. Chem. Soc. Trans. Perkin Trans. 1, 2975 (1994)
InChi	InChI=1S/C24H22N2O4/c1-4-25(5-2)18-10-11-19-15(3)23(24(29)30-20(19)14-18)16-6-8-17(9-7-16)26-21(27)12-13-22(26)28/h6-14H,4-5H2,1-3H3
Quantity	50 mg, 250 mg, Bulk