Crystal Violet

SKU: C0252

Category: Non Fluorescent Detection & Chromogene Detection

Synonyms: Basic Violet 3 , Gentian Violet , Hexamethylpararosaniline chloride , Methyl Violet 10B , C.I. 42555; 548-62-9
CAS-Number: C₂₅H₃₀N₃Cl
Molecular Formula: 407.98
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥88% (dye content)
Identity: IR
Appearance: Dark green powder

Properties

Melting Point: 205 °C (dec.) (lit.)
Solvents: water, chloroform, ethanol

Downloads

Safety Data Sheet: CDX C0252 MSDS.pdf

Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at RT.
Long Term Storage: RT
Short Term Storage: RT
Shipping: AMBIENT

Transportation: Packing Group III
RIDADR: UN3077 9
Signal word: Danger
GHS Symbol: GHS05+GHS07+GHS09
Precautionary statements: P273-P280-P305 + P351 + P338-P501
Hazard statements: H302-H318-H351-H410

Description: Crystal Violet is used as an acid-base indicator. When dissolved in water, the dye has a blue-violet colour with an absorbance maximum at 590nm and an extinction coefficient of 87,000 M?1 cm?1. The colour of the dye depends on the acidity of the solution. At a pH of >1.0, the dye is green with absorption maxima at 420nm and 620nm, while in a strongly acidic solution the dye is yellow with an absorption maximum at 420nm. Spectral data: UV/Visible Absorbance: λ max (water) 584-590nm. Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria. It has been used to check cell viability, for the staining of cells to study cell migration, invasion and cell viability and as a nontoxic DNA stain for DNA visualization in agarose gels. Solutions containing crystal violet and formalin are often used to simultaneously fix and stain cells grown in tissue culture to preserve them and make them easily visible, since most cells are colourless. Crystal violet has antibacterial, antifungal, antihelminthic, antitrypanosomal, antiangiogenic, and antitumor properties. It has shown to calcium-dependent uncouple oxidative phosphorylation. It is used to dye paper, as a component of navy blue and black inks, ball-point pens and inkjet printers. It is also used to colourize diverse products such as fertilizers, antifreezes and detergents. In forensics, gentian violet was used to develop fingerprints.
References: (1) E.Q. Adams & L. Rosenstein, JACS 36, 1452 (1914) , (2) C.E. Hoffmann & O. Rahn, J. Bacteriol. 47, 177 (1944) , (3) H.L. Chance, Stain Technol. 27, 253 (1952) , (4) W.H. Clarke & I.G. Maddocks, Stain Technol. 38, 252 (1963) , (5) M.K. Tolba & A.M. Saleh, Arch. Mikrobiol. 47, 201 (1963) , (6) E. Adams, Stain Technol. 50, 227 (1975) , (7) W. Au, et al., Mutat. Res. 58, 269 (1978) , (8) L.P. Wakelin, et al., Biochemistry 20, 5779 (1981) , (9) S.N. Moreno, et al., J. Biol. Chem. 263, 12493 (1988) , (10) R. Docampo & S.N. Moreno, Drug Metab. Rev. 22, 161 (1990) (Review) , (11) W.J. Bodziak, Forensic Sci. Int. 82, 45 (1996) , (12) Y. Yang, et al., Electrophoresis 22, 855 (2001) , (13) R. Coico, et al., Curr. Protoc. Microbiol. Appendix 3-3C (2005) , (14) V.S. Sukumaran & A. Ramalingam, Spectrochim. Acta A Mol. Biomol. Spectrosc. 63, 673 (2006) , (15) A.M. Maley, et al., Exp. Dermatol. 22, 775 (2013) (Review) , (16) M. Feoktistova, et al., Cold Spring Harb. Protoc. 2016, pdb.prot087379 (2016)
InChi Key: ZXJXZNDDNMQXFV-UHFFFAOYSA-M
Smiles: CN(C)C1=CC=C(/C(C2=CC=C(N(C)C)C=C2)=C3C=C/C(C=C/3)=[N+](C)/C)C=C1.[Cl-]

Crystal Violet is used as an acid-base indicator. When dissolved in water, the dye has a blue-violet colour with an absorbance maximum at 590nm and an extinction coefficient of 87,000 M?1 cm?1. The colour of the dye depends on the acidity of the solution. At a pH of >1.0, the dye is green with absorption maxima at 420nm and 620nm, while in a strongly acidic solution the dye is yellow with an absorption maximum at 420nm. Spectral data: UV/Visible Absorbance: λ max (water) 584-590nm. Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria. It has been used to check cell viability, for the staining of cells to study cell migration, invasion and cell viability and as a nontoxic DNA stain for DNA visualization in agarose gels. Solutions containing crystal violet and formalin are often used to simultaneously fix and stain cells grown in tissue culture to preserve them and make them easily visible, since most cells are colourless. Crystal violet has antibacterial, antifungal, antihelminthic, antitrypanosomal, antiangiogenic, and antitumor properties. It has shown to calcium-dependent uncouple oxidative phosphorylation. It is used to dye paper, as a component of navy blue and black inks, ball-point pens and inkjet printers. It is also used to colourize diverse products such as fertilizers, antifreezes and detergents. In forensics, gentian violet was used to develop fingerprints.

SKU: C0252 Category: Non Fluorescent Detection & Chromogene Detection

Additional information

Additional information

Synonyms	Basic Violet 3, Gentian Violet, Hexamethylpararosaniline chloride, Methyl Violet 10B, C.I. 42555
Purity	≥88% (dye content)
Appearance	Dark green powder
CAS-Number	548-62-9
Molecular Formula	C25H30N3Cl
Molecular Weight	407.98
Identity	IR
Solvents	water, chloroform, ethanol
Melting Point	205 °C (dec.) (lit.)
Smiles	CN(C)C1=CC=C(/C(C2=CC=C(N(C)C)C=C2)=C3C=C/C(C=C/3)=[N+](C)/C)C=C1.[Cl-]
InChi Key	ZXJXZNDDNMQXFV-UHFFFAOYSA-M
Shipping	AMBIENT
Short Term Storage	RT
Long Term Storage	RT
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at RT.
Hazard statements	H302-H318-H351-H410
Precautionary statements	P273-P280-P305 + P351 + P338-P501
GHS Symbol	GHS05+GHS07+GHS09
Signal word	Danger
RIDADR	UN3077 9
Transportation	Packing Group III
References	(1) E.Q. Adams & L. Rosenstein, JACS 36, 1452 (1914), (2) C.E. Hoffmann & O. Rahn, J. Bacteriol. 47, 177 (1944), (3) H.L. Chance, Stain Technol. 27, 253 (1952), (4) W.H. Clarke & I.G. Maddocks, Stain Technol. 38, 252 (1963), (5) M.K. Tolba & A.M. Saleh, Arch. Mikrobiol. 47, 201 (1963), (6) E. Adams, Stain Technol. 50, 227 (1975), (7) W. Au, et al., Mutat. Res. 58, 269 (1978), (8) L.P. Wakelin, et al., Biochemistry 20, 5779 (1981), (9) S.N. Moreno, et al., J. Biol. Chem. 263, 12493 (1988), (10) R. Docampo & S.N. Moreno, Drug Metab. Rev. 22, 161 (1990) (Review), (11) W.J. Bodziak, Forensic Sci. Int. 82, 45 (1996), (12) Y. Yang, et al., Electrophoresis 22, 855 (2001), (13) R. Coico, et al., Curr. Protoc. Microbiol. Appendix 3-3C (2005), (14) V.S. Sukumaran & A. Ramalingam, Spectrochim. Acta A Mol. Biomol. Spectrosc. 63, 673 (2006), (15) A.M. Maley, et al., Exp. Dermatol. 22, 775 (2013) (Review), (16) M. Feoktistova, et al., Cold Spring Harb. Protoc. 2016, pdb.prot087379 (2016)
Quantity	25 g, 100 g, Bulk