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    Crystal Violet

    Available from stock

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      Crystal Violet

      SKU
      C0252

      Category: Non Fluorescent Detection & Chromogene Detection

      Synonyms
      Basic Violet 3 , Gentian Violet , Hexamethylpararosaniline chloride , Methyl Violet 10B , C.I. 42555
      548-62-9
      CAS-Number
      C25H30N3Cl
      Molecular Formula
      407.98
      Molecular Weight

      • Product Data
      • Handling
      • Safety Information
      • Details

      Specifications

      Purity
      ≥88% (dye content)
      Identity
      IR
      Appearance
      Dark green powder

      Properties

      Melting Point
      205 °C (dec.) (lit.)
      Solvents
      water, chloroform, ethanol

      Downloads

      Safety Data Sheet
      CDX C0252 MSDS.pdf
      Handling Advice
      Protect from light and moisture.
      Use / Stability
      Stable for at least 2 years after receipt when stored at RT.
      Long Term Storage
      RT
      Short Term Storage
      RT
      Shipping
      AMBIENT
      Transportation
      Packing Group III
      RIDADR
      UN3077 9
      Signal word
      Danger
      GHS Symbol
      GHS05+GHS07+GHS09
      Precautionary statements
      P273-P280-P305 + P351 + P338-P501
      Hazard statements
      H302-H318-H351-H410
      Description
      Crystal Violet is used as an acid-base indicator. When dissolved in water, the dye has a blue-violet colour with an absorbance maximum at 590nm and an extinction coefficient of 87,000 M?1 cm?1. The colour of the dye depends on the acidity of the solution. At a pH of >1.0, the dye is green with absorption maxima at 420nm and 620nm, while in a strongly acidic solution the dye is yellow with an absorption maximum at 420nm. Spectral data: UV/Visible Absorbance: λ max (water) 584-590nm. Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria. It has been used to check cell viability, for the staining of cells to study cell migration, invasion and cell viability and as a nontoxic DNA stain for DNA visualization in agarose gels. Solutions containing crystal violet and formalin are often used to simultaneously fix and stain cells grown in tissue culture to preserve them and make them easily visible, since most cells are colourless. Crystal violet has antibacterial, antifungal, antihelminthic, antitrypanosomal, antiangiogenic, and antitumor properties. It has shown to calcium-dependent uncouple oxidative phosphorylation. It is used to dye paper, as a component of navy blue and black inks, ball-point pens and inkjet printers. It is also used to colourize diverse products such as fertilizers, antifreezes and detergents. In forensics, gentian violet was used to develop fingerprints.
      References
      (1) E.Q. Adams & L. Rosenstein, JACS 36, 1452 (1914) , (2) C.E. Hoffmann & O. Rahn, J. Bacteriol. 47, 177 (1944) , (3) H.L. Chance, Stain Technol. 27, 253 (1952) , (4) W.H. Clarke & I.G. Maddocks, Stain Technol. 38, 252 (1963) , (5) M.K. Tolba & A.M. Saleh, Arch. Mikrobiol. 47, 201 (1963) , (6) E. Adams, Stain Technol. 50, 227 (1975) , (7) W. Au, et al., Mutat. Res. 58, 269 (1978) , (8) L.P. Wakelin, et al., Biochemistry 20, 5779 (1981) , (9) S.N. Moreno, et al., J. Biol. Chem. 263, 12493 (1988) , (10) R. Docampo & S.N. Moreno, Drug Metab. Rev. 22, 161 (1990) (Review) , (11) W.J. Bodziak, Forensic Sci. Int. 82, 45 (1996) , (12) Y. Yang, et al., Electrophoresis 22, 855 (2001) , (13) R. Coico, et al., Curr. Protoc. Microbiol. Appendix 3-3C (2005) , (14) V.S. Sukumaran & A. Ramalingam, Spectrochim. Acta A Mol. Biomol. Spectrosc. 63, 673 (2006) , (15) A.M. Maley, et al., Exp. Dermatol. 22, 775 (2013) (Review) , (16) M. Feoktistova, et al., Cold Spring Harb. Protoc. 2016, pdb.prot087379 (2016)
      InChi Key
      ZXJXZNDDNMQXFV-UHFFFAOYSA-M
      Smiles
      CN(C)C1=CC=C(/C(C2=CC=C(N(C)C)C=C2)=C3C=C/C(C=C/3)=[N+](C)/C)C=C1.[Cl-]

      Crystal Violet is used as an acid-base indicator. When dissolved in water, the dye has a blue-violet colour with an absorbance maximum at 590nm and an extinction coefficient of 87,000 M?1 cm?1. The colour of the dye depends on the acidity of the solution. At a pH of >1.0, the dye is green with absorption maxima at 420nm and 620nm, while in a strongly acidic solution the dye is yellow with an absorption maximum at 420nm. Spectral data: UV/Visible Absorbance: λ max (water) 584-590nm. Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria. It has been used to check cell viability, for the staining of cells to study cell migration, invasion and cell viability and as a nontoxic DNA stain for DNA visualization in agarose gels. Solutions containing crystal violet and formalin are often used to simultaneously fix and stain cells grown in tissue culture to preserve them and make them easily visible, since most cells are colourless. Crystal violet has antibacterial, antifungal, antihelminthic, antitrypanosomal, antiangiogenic, and antitumor properties. It has shown to calcium-dependent uncouple oxidative phosphorylation. It is used to dye paper, as a component of navy blue and black inks, ball-point pens and inkjet printers. It is also used to colourize diverse products such as fertilizers, antifreezes and detergents. In forensics, gentian violet was used to develop fingerprints.

      SKU: C0252 Category: Non Fluorescent Detection & Chromogene Detection
      • Additional information

      Additional information

      Synonyms

      Basic Violet 3, Gentian Violet, Hexamethylpararosaniline chloride, Methyl Violet 10B, C.I. 42555

      Purity

      ≥88% (dye content)

      Appearance

      Dark green powder

      CAS-Number

      548-62-9

      Molecular Formula

      C25H30N3Cl

      Molecular Weight

      407.98

      Identity

      IR

      Solvents

      water, chloroform, ethanol

      Melting Point

      205 °C (dec.) (lit.)

      Smiles

      CN(C)C1=CC=C(/C(C2=CC=C(N(C)C)C=C2)=C3C=C/C(C=C/3)=[N+](C)/C)C=C1.[Cl-]

      InChi Key

      ZXJXZNDDNMQXFV-UHFFFAOYSA-M

      Shipping

      AMBIENT

      Short Term Storage

      RT

      Long Term Storage

      RT

      Handling Advice

      Protect from light and moisture.

      Use / Stability

      Stable for at least 2 years after receipt when stored at RT.

      Hazard statements

      H302-H318-H351-H410

      Precautionary statements

      P273-P280-P305 + P351 + P338-P501

      GHS Symbol

      GHS05+GHS07+GHS09

      Signal word

      Danger

      RIDADR

      UN3077 9

      Transportation

      Packing Group III

      References

      (1) E.Q. Adams & L. Rosenstein, JACS 36, 1452 (1914), (2) C.E. Hoffmann & O. Rahn, J. Bacteriol. 47, 177 (1944), (3) H.L. Chance, Stain Technol. 27, 253 (1952), (4) W.H. Clarke & I.G. Maddocks, Stain Technol. 38, 252 (1963), (5) M.K. Tolba & A.M. Saleh, Arch. Mikrobiol. 47, 201 (1963), (6) E. Adams, Stain Technol. 50, 227 (1975), (7) W. Au, et al., Mutat. Res. 58, 269 (1978), (8) L.P. Wakelin, et al., Biochemistry 20, 5779 (1981), (9) S.N. Moreno, et al., J. Biol. Chem. 263, 12493 (1988), (10) R. Docampo & S.N. Moreno, Drug Metab. Rev. 22, 161 (1990) (Review), (11) W.J. Bodziak, Forensic Sci. Int. 82, 45 (1996), (12) Y. Yang, et al., Electrophoresis 22, 855 (2001), (13) R. Coico, et al., Curr. Protoc. Microbiol. Appendix 3-3C (2005), (14) V.S. Sukumaran & A. Ramalingam, Spectrochim. Acta A Mol. Biomol. Spectrosc. 63, 673 (2006), (15) A.M. Maley, et al., Exp. Dermatol. 22, 775 (2013) (Review), (16) M. Feoktistova, et al., Cold Spring Harb. Protoc. 2016, pdb.prot087379 (2016)

      Quantity

      25 g, 100 g, Bulk

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