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    Crystal Violet

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    Crystal Violet

    SKU
    C0252

    Category: Non Fluorescent Detection & Chromogene Detection

    Synonyms
    Basic Violet 3 , Gentian Violet , Hexamethylpararosaniline chloride , Methyl Violet 10B , C.I. 42555
    548-62-9
    CAS-Number
    C25H30N3Cl
    Molecular Formula
    407.98
    Molecular Weight

    • Product Data
    • Handling
    • Safety Information
    • Details

    Specifications

    Purity
    ≥88% (dye content)
    Identity
    IR
    Appearance
    Dark green powder

    Properties

    Melting Point
    205 °C (dec.) (lit.)
    Solvents
    water, chloroform, ethanol

    Downloads

    Safety Data Sheet
    CDX C0252 MSDS.pdf
    Handling Advice
    Protect from light and moisture.
    Use / Stability
    Stable for at least 2 years after receipt when stored at RT.
    Long Term Storage
    RT
    Short Term Storage
    RT
    Shipping
    AMBIENT
    Transportation
    Packing Group III
    RIDADR
    UN3077 9
    Signal word
    Danger
    GHS Symbol
    GHS05+GHS07+GHS09
    Precautionary statements
    P273-P280-P305 + P351 + P338-P501
    Hazard statements
    H302-H318-H351-H410
    Description
    Crystal Violet is used as an acid-base indicator. When dissolved in water, the dye has a blue-violet colour with an absorbance maximum at 590nm and an extinction coefficient of 87,000 M?1 cm?1. The colour of the dye depends on the acidity of the solution. At a pH of >1.0, the dye is green with absorption maxima at 420nm and 620nm, while in a strongly acidic solution the dye is yellow with an absorption maximum at 420nm. Spectral data: UV/Visible Absorbance: λ max (water) 584-590nm. Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria. It has been used to check cell viability, for the staining of cells to study cell migration, invasion and cell viability and as a nontoxic DNA stain for DNA visualization in agarose gels. Solutions containing crystal violet and formalin are often used to simultaneously fix and stain cells grown in tissue culture to preserve them and make them easily visible, since most cells are colourless. Crystal violet has antibacterial, antifungal, antihelminthic, antitrypanosomal, antiangiogenic, and antitumor properties. It has shown to calcium-dependent uncouple oxidative phosphorylation. It is used to dye paper, as a component of navy blue and black inks, ball-point pens and inkjet printers. It is also used to colourize diverse products such as fertilizers, antifreezes and detergents. In forensics, gentian violet was used to develop fingerprints.
    References
    (1) E.Q. Adams & L. Rosenstein, JACS 36, 1452 (1914) , (2) C.E. Hoffmann & O. Rahn, J. Bacteriol. 47, 177 (1944) , (3) H.L. Chance, Stain Technol. 27, 253 (1952) , (4) W.H. Clarke & I.G. Maddocks, Stain Technol. 38, 252 (1963) , (5) M.K. Tolba & A.M. Saleh, Arch. Mikrobiol. 47, 201 (1963) , (6) E. Adams, Stain Technol. 50, 227 (1975) , (7) W. Au, et al., Mutat. Res. 58, 269 (1978) , (8) L.P. Wakelin, et al., Biochemistry 20, 5779 (1981) , (9) S.N. Moreno, et al., J. Biol. Chem. 263, 12493 (1988) , (10) R. Docampo & S.N. Moreno, Drug Metab. Rev. 22, 161 (1990) (Review) , (11) W.J. Bodziak, Forensic Sci. Int. 82, 45 (1996) , (12) Y. Yang, et al., Electrophoresis 22, 855 (2001) , (13) R. Coico, et al., Curr. Protoc. Microbiol. Appendix 3-3C (2005) , (14) V.S. Sukumaran & A. Ramalingam, Spectrochim. Acta A Mol. Biomol. Spectrosc. 63, 673 (2006) , (15) A.M. Maley, et al., Exp. Dermatol. 22, 775 (2013) (Review) , (16) M. Feoktistova, et al., Cold Spring Harb. Protoc. 2016, pdb.prot087379 (2016)
    InChi Key
    ZXJXZNDDNMQXFV-UHFFFAOYSA-M
    Smiles
    CN(C)C1=CC=C(/C(C2=CC=C(N(C)C)C=C2)=C3C=C/C(C=C/3)=[N+](C)/C)C=C1.[Cl-]

    Crystal Violet is used as an acid-base indicator. When dissolved in water, the dye has a blue-violet colour with an absorbance maximum at 590nm and an extinction coefficient of 87,000 M?1 cm?1. The colour of the dye depends on the acidity of the solution. At a pH of >1.0, the dye is green with absorption maxima at 420nm and 620nm, while in a strongly acidic solution the dye is yellow with an absorption maximum at 420nm. Spectral data: UV/Visible Absorbance: λ max (water) 584-590nm. Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria. It has been used to check cell viability, for the staining of cells to study cell migration, invasion and cell viability and as a nontoxic DNA stain for DNA visualization in agarose gels. Solutions containing crystal violet and formalin are often used to simultaneously fix and stain cells grown in tissue culture to preserve them and make them easily visible, since most cells are colourless. Crystal violet has antibacterial, antifungal, antihelminthic, antitrypanosomal, antiangiogenic, and antitumor properties. It has shown to calcium-dependent uncouple oxidative phosphorylation. It is used to dye paper, as a component of navy blue and black inks, ball-point pens and inkjet printers. It is also used to colourize diverse products such as fertilizers, antifreezes and detergents. In forensics, gentian violet was used to develop fingerprints.

    SKU: C0252 Category: Non Fluorescent Detection & Chromogene Detection
    • Additional information

    Additional information

    Synonyms

    Basic Violet 3, Gentian Violet, Hexamethylpararosaniline chloride, Methyl Violet 10B, C.I. 42555

    Purity

    ≥88% (dye content)

    Appearance

    Dark green powder

    CAS-Number

    548-62-9

    Molecular Formula

    C25H30N3Cl

    Molecular Weight

    407.98

    Identity

    IR

    Solvents

    water, chloroform, ethanol

    Melting Point

    205 °C (dec.) (lit.)

    Smiles

    CN(C)C1=CC=C(/C(C2=CC=C(N(C)C)C=C2)=C3C=C/C(C=C/3)=[N+](C)/C)C=C1.[Cl-]

    InChi Key

    ZXJXZNDDNMQXFV-UHFFFAOYSA-M

    Shipping

    AMBIENT

    Short Term Storage

    RT

    Long Term Storage

    RT

    Handling Advice

    Protect from light and moisture.

    Use / Stability

    Stable for at least 2 years after receipt when stored at RT.

    Hazard statements

    H302-H318-H351-H410

    Precautionary statements

    P273-P280-P305 + P351 + P338-P501

    GHS Symbol

    GHS05+GHS07+GHS09

    Signal word

    Danger

    RIDADR

    UN3077 9

    Transportation

    Packing Group III

    References

    (1) E.Q. Adams & L. Rosenstein, JACS 36, 1452 (1914), (2) C.E. Hoffmann & O. Rahn, J. Bacteriol. 47, 177 (1944), (3) H.L. Chance, Stain Technol. 27, 253 (1952), (4) W.H. Clarke & I.G. Maddocks, Stain Technol. 38, 252 (1963), (5) M.K. Tolba & A.M. Saleh, Arch. Mikrobiol. 47, 201 (1963), (6) E. Adams, Stain Technol. 50, 227 (1975), (7) W. Au, et al., Mutat. Res. 58, 269 (1978), (8) L.P. Wakelin, et al., Biochemistry 20, 5779 (1981), (9) S.N. Moreno, et al., J. Biol. Chem. 263, 12493 (1988), (10) R. Docampo & S.N. Moreno, Drug Metab. Rev. 22, 161 (1990) (Review), (11) W.J. Bodziak, Forensic Sci. Int. 82, 45 (1996), (12) Y. Yang, et al., Electrophoresis 22, 855 (2001), (13) R. Coico, et al., Curr. Protoc. Microbiol. Appendix 3-3C (2005), (14) V.S. Sukumaran & A. Ramalingam, Spectrochim. Acta A Mol. Biomol. Spectrosc. 63, 673 (2006), (15) A.M. Maley, et al., Exp. Dermatol. 22, 775 (2013) (Review), (16) M. Feoktistova, et al., Cold Spring Harb. Protoc. 2016, pdb.prot087379 (2016)

    Quantity

    25 g, 100 g, Bulk

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