Available from stock

DACM

SKU: D0047

Categories: Fluorescent Detection, Fluorescent Detection

Synonyms: N-(7-Dimethylamino-4-methylcoumarin-3-yl) maleimide; 55145-14-7
CAS-Number: C₁₆H₁₄N₂O₄
Molecular Formula: 298.29
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥98% (NMR)
Appearance: Orange solid
Identity: 1H-NMR

Properties

Solvents: water
Melting Point: 218-220 °C
Fluorescence: λex 398 nm, λem 482 nm in 0.1 M phosphate pH 7.0 (after derivatization with 2-mercaptoethanol)

Shipping: AMBIENT
Short Term Storage: +4°C
Long Term Storage: -20°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.

Hazard statements: H315, H335
Precautionary statements: P261
GHS Symbol: GHS07
Signal word: Warning
Transportation: Not dangerous goods

Description: Excellent blue fluorescent thiol-reactive reagent (Ex/Em: 383/463nm), widely used for probing configurations of biomolecules such as proteins and create blue-fluorescent bioconjugates. The unreacted reagent is non-fluorescent and can also be used to quantitate free thiols. Shows superior fluorescence and water solubility properties than dansyl chloride.
Smiles: CN(C)C1=CC=C2C(OC(=O)C(N3C(=O)C=CC3=O)=C2C)=C1
InChi Key: ADEORFBTPGKHRP-UHFFFAOYSA-N
References: (1) L. Pollegioni et al.: Arch. of Biochem. Bioph. 343(1), 1 (1997) , (2) B. Werneburg et al.: Arch. of Biochem. Bioph. 303(2), 214 (1993) , (3) Y. Kanaoka et al.: Angew. Chem. 89, 141 (1977)
InChi: InChI=1S/C16H14N2O4/c1-9-11-5-4-10(17(2)3)8-12(11)22-16(21)15(9)18-13(19)6-7-14(18)20/h4-8H,1-3H3

Excellent blue fluorescent thiol-reactive reagent (Ex/Em: 383/463nm), widely used for probing configurations of biomolecules such as proteins and create blue-fluorescent bioconjugates. The unreacted reagent is non-fluorescent and can also be used to quantitate free thiols. Shows superior fluorescence and water solubility properties than dansyl chloride.

SKU: D0047 Categories: Fluorescent Detection, Fluorescent Detection

Additional information

Additional information

Synonyms	N-(7-Dimethylamino-4-methylcoumarin-3-yl) maleimide
Purity	≥98% (NMR)
Appearance	Orange solid
CAS-Number	55145-14-7
Molecular Formula	C16H14N2O4
Molecular Weight	298.29
Identity	1H-NMR
Solvents	water
Melting Point	218-220 °C
Fluorescence	λex 398 nm, λem 482 nm in 0.1 M phosphate pH 7.0 (after derivatization with 2-mercaptoethanol)
Smiles	CN(C)C1=CC=C2C(OC(=O)C(N3C(=O)C=CC3=O)=C2C)=C1
InChi Key	ADEORFBTPGKHRP-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Hazard statements	H315, H335
Precautionary statements	P261
GHS Symbol	GHS07
Signal word	Warning
Transportation	Not dangerous goods
References	(1) L. Pollegioni et al.: Arch. of Biochem. Bioph. 343(1), 1 (1997), (2) B. Werneburg et al.: Arch. of Biochem. Bioph. 303(2), 214 (1993), (3) Y. Kanaoka et al.: Angew. Chem. 89, 141 (1977)
InChi	InChI=1S/C16H14N2O4/c1-9-11-5-4-10(17(2)3)8-12(11)22-16(21)15(9)18-13(19)6-7-14(18)20/h4-8H,1-3H3
Quantity	5 mg, 25 mg, Bulk