Specifications
- Purity
- ≥98% (NMR)
- Identity
- 1H-NMR
- Appearance
- Orange solid
Properties
- Fluorescence
- λex 398 nm, λem 482 nm in 0.1 M phosphate pH 7.0 (after derivatization with 2-mercaptoethanol)
- Melting Point
- 218-220 °C
- Solvents
- water
Categories: Fluorescent Detection, Fluorescent Detection
Excellent blue fluorescent thiol-reactive reagent (Ex/Em: 383/463nm), widely used for probing configurations of biomolecules such as proteins and create blue-fluorescent bioconjugates. The unreacted reagent is non-fluorescent and can also be used to quantitate free thiols. Shows superior fluorescence and water solubility properties than dansyl chloride.
Synonyms | N-(7-Dimethylamino-4-methylcoumarin-3-yl) maleimide |
---|---|
Purity | ≥98% (NMR) |
Appearance | Orange solid |
CAS-Number | 55145-14-7 |
Molecular Formula | C16H14N2O4 |
Molecular Weight | 298.29 |
Identity | 1H-NMR |
Solvents | water |
Melting Point | 218-220 °C |
Fluorescence | λex 398 nm, λem 482 nm in 0.1 M phosphate pH 7.0 (after derivatization with 2-mercaptoethanol) |
Smiles | CN(C)C1=CC=C2C(OC(=O)C(N3C(=O)C=CC3=O)=C2C)=C1 |
InChi Key | ADEORFBTPGKHRP-UHFFFAOYSA-N |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
Hazard statements | H315, H335 |
Precautionary statements | P261 |
GHS Symbol | GHS07 |
Signal word | Warning |
Transportation | Not dangerous goods |
References | (1) L. Pollegioni et al.: Arch. of Biochem. Bioph. 343(1), 1 (1997), (2) B. Werneburg et al.: Arch. of Biochem. Bioph. 303(2), 214 (1993), (3) Y. Kanaoka et al.: Angew. Chem. 89, 141 (1977) |
InChi | InChI=1S/C16H14N2O4/c1-9-11-5-4-10(17(2)3)8-12(11)22-16(21)15(9)18-13(19)6-7-14(18)20/h4-8H,1-3H3 |
Quantity | 5 mg, 25 mg, Bulk |