Specifications
- Purity
- ≥95% (HPLC)
- Appearance
- Liquid
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO
- Fluorescence
- λex 491 nm, λem 513 nm
Available from stock
DAF-2 DA solution (5 mM in DMSO), 1 mg in 0.45 ml DMSO
- SKU
- D0585
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 4,5-Diaminofluorescein diacetate , 5,6-Diaminofluorescein diacetate
- 205391-02-2
- CAS-Number
- C24H18N2O7
- Molecular Formula
- 446.41
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Transportation
- Not dangerous goods
- Description
- DAF-2 DA is probably the most successful indicator for nitric oxide. As with other fluorescein diacetates, DAF-2 diacetate is membrane permeant and is deacetylated by intracellular esterases to 4,5-diaminofluorescein (DAF-2). Can be used for the fluorimetric detection of nitric oxide and in fluorescence microscopy to measure real-time changes in nitric oxide levels in vivo.
- Smiles
- CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=C(N)C(N)=C3)C1=CC=C(OC(C)=O)C=C1O2
- InChi Key
- PTSUYDXEEKDBQU-UHFFFAOYSA-N
- References
- (1) V.M. Dirsch et al., Biol. Proc. Online 5(1), 136 (2003) , (2) N. Nagata et al., J. Biochem. 125(4), 658 (1999) , (3) H. Kojima et al., Anal. Chem. 70(13), 2446 (1998) , (4) H. Kojima et al., Chem. Pharm. Bull. 46, 373 (1998) , (5) H. Kojima et al., Neuroreport 9, 3345 (1998)
- InChi
- InChI=1S/C24H18N2O7/c1-11(27)30-13-3-5-16-21(7-13)32-22-8-14(31-12(2)28)4-6-17(22)24(16)18-10-20(26)19(25)9-15(18)23(29)33-24/h3-10H,25-26H2,1-2H3
DAF-2 DA is probably the most successful indicator for nitric oxide. As with other fluorescein diacetates, DAF-2 diacetate is membrane permeant and is deacetylated by intracellular esterases to 4,5-diaminofluorescein (DAF-2). Can be used for the fluorimetric detection of nitric oxide and in fluorescence microscopy to measure real-time changes in nitric oxide levels in vivo.
Additional information
| Synonyms | 4,5-Diaminofluorescein diacetate, 5,6-Diaminofluorescein diacetate |
|---|---|
| Purity | ≥95% (HPLC) |
| Appearance | Liquid |
| CAS-Number | 205391-02-2 |
| Molecular Formula | C24H18N2O7 |
| Molecular Weight | 446.41 |
| Identity | 1H-NMR |
| Solvents | DMSO |
| Fluorescence | λex 491 nm, λem 513 nm |
| Smiles | CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=C(N)C(N)=C3)C1=CC=C(OC(C)=O)C=C1O2 |
| InChi Key | PTSUYDXEEKDBQU-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Transportation | Not dangerous goods |
| References | (1) V.M. Dirsch et al., Biol. Proc. Online 5(1), 136 (2003), (2) N. Nagata et al., J. Biochem. 125(4), 658 (1999), (3) H. Kojima et al., Anal. Chem. 70(13), 2446 (1998), (4) H. Kojima et al., Chem. Pharm. Bull. 46, 373 (1998), (5) H. Kojima et al., Neuroreport 9, 3345 (1998) |
| InChi | InChI=1S/C24H18N2O7/c1-11(27)30-13-3-5-16-21(7-13)32-22-8-14(31-12(2)28)4-6-17(22)24(16)18-10-20(26)19(25)9-15(18)23(29)33-24/h3-10H,25-26H2,1-2H3 |
| Quantity | 1 mg, Bulk |
