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    DAF-2 DA

    Available from stock

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      DAF-2 DA

      SKU
      D0085

      Categories: Fluorescent Detection, Fluorescent Detection

      Synonyms
      4,5-Diaminofluorescein diacetate , 5,6-Diaminofluorescein diacetate
      205391-02-2
      CAS-Number
      C24H18N2O7
      Molecular Formula
      446.41
      Molecular Weight

      For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

      • Product Data
      • Handling
      • Safety Information
      • Details

      Specifications

      Purity
      ≥95% (HPLC)
      Appearance
      Off-white powder
      Identity
      1H-NMR

      Properties

      Solvents
      DMSO
      Fluorescence
      λex 491 nm, λem 513 nm

      Documentation

      Safety Data Sheet (SDS)
      CDX D0085 MSDS.pdf
      Shipping
      AMBIENT
      Short Term Storage
      +4°C
      Long Term Storage
      -20°C
      Handling Advice
      Protect from light and moisture.
      Use / Stability
      Stable for at least 2 years after receipt when stored at -20°C.
      Transportation
      Not dangerous goods
      Description
      DAF-2 DA is probably the most successful indicator for nitric oxide. As with other fluorescein diacetates, DAF-2 diacetate is membrane permeant and is deacetylated by intracellular esterases to 4,5-diaminofluorescein (DAF-2). Can be used for the fluorimetric detection of nitric oxide and in fluorescence microscopy to measure real-time changes in nitric oxide levels in vivo.
      Smiles
      CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=C(N)C(N)=C3)C1=CC=C(OC(C)=O)C=C1O2
      InChi Key
      PTSUYDXEEKDBQU-UHFFFAOYSA-N
      References
      (1) V.M. Dirsch et al.: Biol. Proc. Online 5(1), 136 (2003) , (2) N. Nagata et al.: J. Biochem. 125(4), 658 (1999) , (3) H. Kojima et al.: Anal. Chem. 70(13), 2446 (1998) , (4) H. Kojima et al.: Chem. Pharm. Bull. 46, 373 (1998) , (5) H. Kojima et al.: Neuroreport 9, 3345 (1998)
      InChi
      InChI=1S/C24H18N2O7/c1-11(27)30-13-3-5-16-21(7-13)32-22-8-14(31-12(2)28)4-6-17(22)24(16)18-10-20(26)19(25)9-15(18)23(29)33-24/h3-10H,25-26H2,1-2H3

      DAF-2 DA is probably the most successful indicator for nitric oxide. As with other fluorescein diacetates, DAF-2 diacetate is membrane permeant and is deacetylated by intracellular esterases to 4,5-diaminofluorescein (DAF-2). Can be used for the fluorimetric detection of nitric oxide and in fluorescence microscopy to measure real-time changes in nitric oxide levels in vivo.

      SKU: D0085 Categories: Fluorescent Detection, Fluorescent Detection
      • Additional information

      Additional information

      Synonyms

      4,5-Diaminofluorescein diacetate, 5,6-Diaminofluorescein diacetate

      Purity

      ≥95% (HPLC)

      Appearance

      Off-white powder

      CAS-Number

      205391-02-2

      Molecular Formula

      C24H18N2O7

      Molecular Weight

      446.41

      Identity

      1H-NMR

      Solvents

      DMSO

      Fluorescence

      λex 491 nm, λem 513 nm

      Smiles

      CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=C(N)C(N)=C3)C1=CC=C(OC(C)=O)C=C1O2

      InChi Key

      PTSUYDXEEKDBQU-UHFFFAOYSA-N

      Shipping

      AMBIENT

      Short Term Storage

      +4°C

      Long Term Storage

      -20°C

      Handling Advice

      Protect from light and moisture.

      Use / Stability

      Stable for at least 2 years after receipt when stored at -20°C.

      Transportation

      Not dangerous goods

      References

      (1) V.M. Dirsch et al.: Biol. Proc. Online 5(1), 136 (2003), (2) N. Nagata et al.: J. Biochem. 125(4), 658 (1999), (3) H. Kojima et al.: Anal. Chem. 70(13), 2446 (1998), (4) H. Kojima et al.: Chem. Pharm. Bull. 46, 373 (1998), (5) H. Kojima et al.: Neuroreport 9, 3345 (1998)

      InChi

      InChI=1S/C24H18N2O7/c1-11(27)30-13-3-5-16-21(7-13)32-22-8-14(31-12(2)28)4-6-17(22)24(16)18-10-20(26)19(25)9-15(18)23(29)33-24/h3-10H,25-26H2,1-2H3

      Quantity

      1 mg, 5 mg, 10 mg, Bulk

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