Specifications
- Purity
- ≥95% (HPLC)
- Identity
- 1H-NMR
- Appearance
- Off-white powder
Properties
- Fluorescence
- λex 491 nm, λem 513 nm
- Solvents
- DMSO
Available from stock
DAF-2 DA
- SKU
- D0085
Category: Fluorescent Detection
- Synonyms
- 4,5-Diaminofluorescein diacetate , 5,6-Diaminofluorescein diacetate
- 205391-02-2
- CAS-Number
- C24H18N2O7
- Molecular Formula
- 446.41
- Molecular Weight
- Short Term Storage
- +4°C
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Handling Advice
- Protect from light and moisture.
- Long Term Storage
- -20°C
- Shipping
- AMBIENT
- Transportation
- Not dangerous goods
- Description
- DAF-2 DA is probably the most successful indicator for nitric oxide. As with other fluorescein diacetates, DAF-2 diacetate is membrane permeant and is deacetylated by intracellular esterases to 4,5-diaminofluorescein (DAF-2). Can be used for the fluorimetric detection of nitric oxide and in fluorescence microscopy to measure real-time changes in nitric oxide levels in vivo.
- InChi
- InChI=1S/C24H18N2O7/c1-11(27)30-13-3-5-16-21(7-13)32-22-8-14(31-12(2)28)4-6-17(22)24(16)18-10-20(26)19(25)9-15(18)23(29)33-24/h3-10H,25-26H2,1-2H3
- References
- (1) V.M. Dirsch et al.: Biol. Proc. Online 5(1), 136 (2003) , (2) N. Nagata et al.: J. Biochem. 125(4), 658 (1999) , (3) H. Kojima et al.: Anal. Chem. 70(13), 2446 (1998) , (4) H. Kojima et al.: Chem. Pharm. Bull. 46, 373 (1998) , (5) H. Kojima et al.: Neuroreport 9, 3345 (1998)
- InChi Key
- PTSUYDXEEKDBQU-UHFFFAOYSA-N
- Smiles
- CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=C(N)C(N)=C3)C1=CC=C(OC(C)=O)C=C1O2
DAF-2 DA is probably the most successful indicator for nitric oxide. As with other fluorescein diacetates, DAF-2 diacetate is membrane permeant and is deacetylated by intracellular esterases to 4,5-diaminofluorescein (DAF-2). Can be used for the fluorimetric detection of nitric oxide and in fluorescence microscopy to measure real-time changes in nitric oxide levels in vivo.
Additional information
| Synonyms | 4,5-Diaminofluorescein diacetate, 5,6-Diaminofluorescein diacetate |
|---|---|
| Purity | ≥95% (HPLC) |
| Appearance | Off-white powder |
| CAS-Number | 205391-02-2 |
| Molecular Formula | C24H18N2O7 |
| Molecular Weight | 446.41 |
| Identity | 1H-NMR |
| Solvents | DMSO |
| Fluorescence | λex 491 nm, λem 513 nm |
| Smiles | CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=C(N)C(N)=C3)C1=CC=C(OC(C)=O)C=C1O2 |
| InChi Key | PTSUYDXEEKDBQU-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Transportation | Not dangerous goods |
| References | (1) V.M. Dirsch et al.: Biol. Proc. Online 5(1), 136 (2003), (2) N. Nagata et al.: J. Biochem. 125(4), 658 (1999), (3) H. Kojima et al.: Anal. Chem. 70(13), 2446 (1998), (4) H. Kojima et al.: Chem. Pharm. Bull. 46, 373 (1998), (5) H. Kojima et al.: Neuroreport 9, 3345 (1998) |
| InChi | InChI=1S/C24H18N2O7/c1-11(27)30-13-3-5-16-21(7-13)32-22-8-14(31-12(2)28)4-6-17(22)24(16)18-10-20(26)19(25)9-15(18)23(29)33-24/h3-10H,25-26H2,1-2H3 |
| Quantity | 1 mg, 5 mg, 10 mg, Bulk |
