- Synonyms: 4,5-Diaminofluorescein diacetate, 5,6-Diaminofluorescein diacetate
- Purity: ≥95% (HPLC)
- Appearance: Off-white powder.
- CAS-Number: 205391-02-2
- Molecular Formula: C24H18N2O7
- Molecular Weight: 446.41
- Identity: 1H-NMR
- Solvents: DMSO
- Fluorescence: λex 491 nm; λem 513 nm
- Smiles: CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=C(N)C(N)=C3)C1=CC=C(OC(C)=O)C=C1O2
- InChi: InChI=1S/C24H18N2O7/c1-11(27)30-13-3-5-16-21(7-13)32-22-8-14(31-12(2)28)4-6-17(22)24(16)18-10-20(26)19(25)9-15(18)23(29)33-24/h3-10H,25-26H2,1-2H3
- InChi Key: PTSUYDXEEKDBQU-UHFFFAOYSA-N
Available from stock
DAF-2 DA
SKU: D0085
Category: Fluorescent Detection
- Shipping: AMBIENT
- Short Term Storage: +4°C
- Long Term Storage: -20°C
- Handling Advice: Protect from light and moisture.
- Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.
- Description: DAF-2 DA is probably the most successful indicator for nitric oxide. As with other fluorescein diacetates, DAF-2 diacetate is membrane permeant and is deacetylated by intracellular esterases to 4,5-diaminofluorescein (DAF-2). Can be used for the fluorimetric detection of nitric oxide and in fluorescence microscopy to measure real-time changes in nitric oxide levels in vivo.
- References: (1) V.M. Dirsch et al.; Biol. Proc. Online 5(1), 136 (2003), (2) N. Nagata et al.; J. Biochem. 125(4), 658 (1999), (3) H. Kojima et al.; Anal. Chem. 70(13), 2446 (1998), (4) H. Kojima et al.; Chem. Pharm. Bull. 46, 373 (1998), (5) H. Kojima et al.; Neuroreport 9, 3345 (1998)
DAF-2 DA is probably the most successful indicator for nitric oxide. As with other fluorescein diacetates, DAF-2 diacetate is membrane permeant and is deacetylated by intracellular esterases to 4,5-diaminofluorescein (DAF-2). Can be used for the fluorimetric detection of nitric oxide and in fluorescence microscopy to measure real-time changes in nitric oxide levels in vivo.
Additional information
| Identity | 1H-NMR |
|---|---|
| Handling Advice | Protect from light and moisture. |
| Availability | 1 |
| CAS-Number | 205391-02-2 |
| Long Term Storage | -20°C |
| InChi Key | PTSUYDXEEKDBQU-UHFFFAOYSA-N |
| Quantity | 1 mg, 10 mg, 5 mg, Bulk |
| Appearance | Off-white powder. |
| InChi | InChI=1S/C24H18N2O7/c1-11(27)30-13-3-5-16-21(7-13)32-22-8-14(31-12(2)28)4-6-17(22)24(16)18-10-20(26)19(25)9-15(18)23(29)33-24/h3-10H,25-26H2,1-2H3 |
| Fluorescence | λex 491 nm; λem 513 nm |
| Molecular Formula | |
| Short Term Storage | +4°C |
| Purity | |
| Pricetable | |
| References | (4) H. Kojima et al.; Chem. Pharm. Bull. 46, 373 (1998), (1) V.M. Dirsch et al.; Biol. Proc. Online 5(1), 136 (2003), (2) N. Nagata et al.; J. Biochem. 125(4), 658 (1999), (3) H. Kojima et al.; Anal. Chem. 70(13), 2446 (1998), (5) H. Kojima et al.; Neuroreport 9, 3345 (1998) |
| Smiles | CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=C(N)C(N)=C3)C1=CC=C(OC(C)=O)C=C1O2 |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Molecular Weight | |
| Solvents | DMSO |
| Synonyms | 4,5-Diaminofluorescein diacetate, 5,6-Diaminofluorescein diacetate |
| Shipping | AMBIENT |
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