Specifications
- Purity
- ≥95% (HPLC)
- Appearance
- Liquid
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO
- Fluorescence
- λex 505 nm, λem 518 nm in 0.1 M Tris pH 8.0
Available from stock
DAF-2 solution (5 mM in DMSO), 1 mg in 0.55 ml DMSO
- SKU
- D0584
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 4,5-Diaminofluorescein
- 205391-01-1
- CAS-Number
- C20H14N2O5
- Molecular Formula
- 362.34
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Hazard statements
- H302
- GHS Symbol
- GHS07
- Signal word
- Warning
- Transportation
- Not dangerous goods
- Description
- DAF-2 is highly sensitive reagent for NO detection and determination of nitric oxide synthase activity. DAF-2, however, remains essentially nonfluorescent until it reacts with the nitrosonium cation (produced by spontaneous oxidation of nitric oxide) to form a fluorescent heterocycle, which becomes trapped in the cell's cytoplasm. This sensitive fluorescent probe has been used to identify individual nitric oxide-producing neurons in brain slices, in mitochondria and in living plant cells.
- Smiles
- NC1=CC2=C(C=C1N)C1(OC2=O)C2=CC=C(O)C=C2OC2=C1C=CC(O)=C2
- InChi Key
- LTYUPYUWXRTNFQ-UHFFFAOYSA-N
- References
- (1) M. Kirsch et al., J. Pineal Res. 40(1), 10 (2006) , (2) V.M. Dirsch et al., Biol. Proc. Online 5(1), 136 (2003) , (3) N. Nagata et al., J. Biochem. 125(4), 658 (1999) , (4) H. Kojima et al., Chem. Pharm. Bull. 46, 373 (1998) , (5) H. Kojima et al., Anal. Chem. 70, 2446 (1998)
- InChi
- InChI=1S/C20H14N2O5/c21-15-7-11-14(8-16(15)22)20(27-19(11)25)12-3-1-9(23)5-17(12)26-18-6-10(24)2-4-13(18)20/h1-8,23-24H,21-22H2
DAF-2 is highly sensitive reagent for NO detection and determination of nitric oxide synthase activity. DAF-2, however, remains essentially nonfluorescent until it reacts with the nitrosonium cation (produced by spontaneous oxidation of nitric oxide) to form a fluorescent heterocycle, which becomes trapped in the cell’s cytoplasm. This sensitive fluorescent probe has been used to identify individual nitric oxide-producing neurons in brain slices, in mitochondria and in living plant cells.
Additional information
| Synonyms | 4,5-Diaminofluorescein |
|---|---|
| Purity | ≥95% (HPLC) |
| Appearance | Liquid |
| CAS-Number | 205391-01-1 |
| Molecular Formula | C20H14N2O5 |
| Molecular Weight | 362.34 |
| Identity | 1H-NMR |
| Solvents | DMSO |
| Fluorescence | λex 505 nm, λem 518 nm in 0.1 M Tris pH 8.0 |
| Smiles | NC1=CC2=C(C=C1N)C1(OC2=O)C2=CC=C(O)C=C2OC2=C1C=CC(O)=C2 |
| InChi Key | LTYUPYUWXRTNFQ-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Hazard statements | H302 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) M. Kirsch et al., J. Pineal Res. 40(1), 10 (2006), (2) V.M. Dirsch et al., Biol. Proc. Online 5(1), 136 (2003), (3) N. Nagata et al., J. Biochem. 125(4), 658 (1999), (4) H. Kojima et al., Chem. Pharm. Bull. 46, 373 (1998), (5) H. Kojima et al., Anal. Chem. 70, 2446 (1998) |
| InChi | InChI=1S/C20H14N2O5/c21-15-7-11-14(8-16(15)22)20(27-19(11)25)12-3-1-9(23)5-17(12)26-18-6-10(24)2-4-13(18)20/h1-8,23-24H,21-22H2 |
| Quantity | 1 mg, Bulk |
