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    DAF-4 DA

    Available from stock

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      DAF-4 DA

      SKU
      D0216

      Category: Fluorescent Detection

      Synonyms
      DAF-4 diacetate , 3,4-Diamino-2',7'-dichlorofluorescein diacetate
      208850-34-4
      CAS-Number
      C24H16Cl2N2O7
      Molecular Formula
      515.30
      Molecular Weight

      • Product Data
      • Handling
      • Safety Information
      • Details

      Specifications

      Purity
      ≥90% (HPLC)
      Identity
      1H-NMR
      Appearance
      Light yellow powder

      Properties

      Solvents
      chloroform, DMSO
      Short Term Storage
      +4°C
      Use / Stability
      Stable for at least 2 years after receipt when stored at -20°C.
      Handling Advice
      Protect from light and moisture.
      Long Term Storage
      -20°C
      Shipping
      AMBIENT
      Transportation
      Not dangerous goods
      Description
      DAF-4 is a indicator for nitric oxide. As with other fluorescein diacetates (DAFs), the non-fluorescent DAF-4 diacetate (DAF-4 DA) is membrane permeant and is deacetylated by intracellular esterases to form DAF-4, which than can ract with NO to form the fluorescent DAF-4T. Can be used for the fluorimetric detection of nitric oxide and in fluorescence microscopy to measure real-time changes in nitric oxide levels in vivo. Spectral Data: λex 505nm, λem 530nm.
      InChi
      InChI=1S/C24H16Cl2N2O7/c1-9(29)32-21-7-19-13(4-15(21)25)24(12-6-18(28)17(27)3-11(12)23(31)35-24)14-5-16(26)22(33-10(2)30)8-20(14)34-19/h3-8H,27-28H2,1-2H3
      References
      (1) H. Kojima, et al., Anal. Chem. 70, 2446 (1998) , (2) N. Nakatsubo, et al., FEBS Lett. 427, 263 (1998) , (3) T. Nagano, Luminescence 14, 283 (1999) (Review) , (4) T. Nagano, Proc. Jpn. Acad. Ser. B 86, 837 (2010) (Review)
      InChi Key
      OOSGYDWTQGDKAY-UHFFFAOYSA-N
      Smiles
      ClC1=C(OC(C)=O)C=C(OC(C=C(OC(C)=O)C(Cl)=C2)=C2C34OC(C5=C4C=C(N)C(N)=C5)=O)C3=C1

      DAF-4 is a indicator for nitric oxide. As with other fluorescein diacetates (DAFs), the non-fluorescent DAF-4 diacetate (DAF-4 DA) is membrane permeant and is deacetylated by intracellular esterases to form DAF-4, which than can ract with NO to form the fluorescent DAF-4T. Can be used for the fluorimetric detection of nitric oxide and in fluorescence microscopy to measure real-time changes in nitric oxide levels in vivo. Spectral Data: λex 505nm, λem 530nm.

      SKU: D0216 Category: Fluorescent Detection
      • Additional information

      Additional information

      Synonyms

      DAF-4 diacetate, 3,4-Diamino-2',7'-dichlorofluorescein diacetate

      Purity

      ≥90% (HPLC)

      Appearance

      Light yellow powder

      CAS-Number

      208850-34-4

      Molecular Formula

      C24H16Cl2N2O7

      Molecular Weight

      515.30

      Identity

      1H-NMR

      Solvents

      chloroform, DMSO

      Smiles

      ClC1=C(OC(C)=O)C=C(OC(C=C(OC(C)=O)C(Cl)=C2)=C2C34OC(C5=C4C=C(N)C(N)=C5)=O)C3=C1

      InChi Key

      OOSGYDWTQGDKAY-UHFFFAOYSA-N

      Shipping

      AMBIENT

      Short Term Storage

      +4°C

      Long Term Storage

      -20°C

      Handling Advice

      Protect from light and moisture.

      Use / Stability

      Stable for at least 2 years after receipt when stored at -20°C.

      Transportation

      Not dangerous goods

      References

      (1) H. Kojima, et al., Anal. Chem. 70, 2446 (1998), (2) N. Nakatsubo, et al., FEBS Lett. 427, 263 (1998), (3) T. Nagano, Luminescence 14, 283 (1999) (Review), (4) T. Nagano, Proc. Jpn. Acad. Ser. B 86, 837 (2010) (Review)

      InChi

      InChI=1S/C24H16Cl2N2O7/c1-9(29)32-21-7-19-13(4-15(21)25)24(12-6-18(28)17(27)3-11(12)23(31)35-24)14-5-16(26)22(33-10(2)30)8-20(14)34-19/h3-8H,27-28H2,1-2H3

      Quantity

      1 mg, 5 mg, 10 mg, Bulk

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