DAF-4 DA

SKU: D0216

Category: Fluorescent Detection

Synonyms: DAF-4 diacetate , 3,4-Diamino-2',7'-dichlorofluorescein diacetate; 208850-34-4
CAS-Number: C₂₄H₁₆Cl₂N₂O₇
Molecular Formula: 515.30
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥90% (HPLC)
Identity: 1H-NMR
Appearance: Light yellow powder

Properties

Solvents: chloroform, DMSO

Short Term Storage: +4°C
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.
Handling Advice: Protect from light and moisture.
Long Term Storage: -20°C
Shipping: AMBIENT

Transportation: Not dangerous goods

Description: DAF-4 is a indicator for nitric oxide. As with other fluorescein diacetates (DAFs), the non-fluorescent DAF-4 diacetate (DAF-4 DA) is membrane permeant and is deacetylated by intracellular esterases to form DAF-4, which than can ract with NO to form the fluorescent DAF-4T. Can be used for the fluorimetric detection of nitric oxide and in fluorescence microscopy to measure real-time changes in nitric oxide levels in vivo. Spectral Data: λex 505nm, λem 530nm.
InChi: InChI=1S/C24H16Cl2N2O7/c1-9(29)32-21-7-19-13(4-15(21)25)24(12-6-18(28)17(27)3-11(12)23(31)35-24)14-5-16(26)22(33-10(2)30)8-20(14)34-19/h3-8H,27-28H2,1-2H3
References: (1) H. Kojima, et al., Anal. Chem. 70, 2446 (1998) , (2) N. Nakatsubo, et al., FEBS Lett. 427, 263 (1998) , (3) T. Nagano, Luminescence 14, 283 (1999) (Review) , (4) T. Nagano, Proc. Jpn. Acad. Ser. B 86, 837 (2010) (Review)
InChi Key: OOSGYDWTQGDKAY-UHFFFAOYSA-N
Smiles: ClC1=C(OC(C)=O)C=C(OC(C=C(OC(C)=O)C(Cl)=C2)=C2C34OC(C5=C4C=C(N)C(N)=C5)=O)C3=C1

DAF-4 is a indicator for nitric oxide. As with other fluorescein diacetates (DAFs), the non-fluorescent DAF-4 diacetate (DAF-4 DA) is membrane permeant and is deacetylated by intracellular esterases to form DAF-4, which than can ract with NO to form the fluorescent DAF-4T. Can be used for the fluorimetric detection of nitric oxide and in fluorescence microscopy to measure real-time changes in nitric oxide levels in vivo. Spectral Data: λex 505nm, λem 530nm.

SKU: D0216 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	DAF-4 diacetate, 3,4-Diamino-2',7'-dichlorofluorescein diacetate
Purity	≥90% (HPLC)
Appearance	Light yellow powder
CAS-Number	208850-34-4
Molecular Formula	C24H16Cl2N2O7
Molecular Weight	515.30
Identity	1H-NMR
Solvents	chloroform, DMSO
Smiles	ClC1=C(OC(C)=O)C=C(OC(C=C(OC(C)=O)C(Cl)=C2)=C2C34OC(C5=C4C=C(N)C(N)=C5)=O)C3=C1
InChi Key	OOSGYDWTQGDKAY-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Transportation	Not dangerous goods
References	(1) H. Kojima, et al., Anal. Chem. 70, 2446 (1998), (2) N. Nakatsubo, et al., FEBS Lett. 427, 263 (1998), (3) T. Nagano, Luminescence 14, 283 (1999) (Review), (4) T. Nagano, Proc. Jpn. Acad. Ser. B 86, 837 (2010) (Review)
InChi	InChI=1S/C24H16Cl2N2O7/c1-9(29)32-21-7-19-13(4-15(21)25)24(12-6-18(28)17(27)3-11(12)23(31)35-24)14-5-16(26)22(33-10(2)30)8-20(14)34-19/h3-8H,27-28H2,1-2H3
Quantity	1 mg, 5 mg, 10 mg, Bulk