Specifications
- Purity
- ≥95% (HPLC)
- Appearance
- Yellow powder
- Identity
- 1H-NMR
Properties
- Solvents
- water (10 mg/ml,warming), DMSO, methanol or ethanol (slightly)
- Melting Point
- 330°C (dec.)
- Other
- pH: 4.0-5.0 at 10 g/l at 20°C
Available from stock
DAPI dihydrochloride
- SKU
- D0025
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride , 4',6-Diamidino-2-phenylindole dihydrochloride , FxCycle Violet
- 28718-90-3
- CAS-Number
- C16H15N5 · 2HCl
- Molecular Formula
- 350.25
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- +4°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Hazard statements
- H315, H319, H335
- Precautionary statements
- P261, P271, P280
- GHS Symbol
- GHS07
- Transportation
- Not dangerous goods
- Description
- DAPI (4?,6-Diamidino-2-phenylindole dihydrochloride) is a cell permeable, fluorescent dye that is a minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone. DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma. Spectral Data: Excitation: λex 340 nm, Emission: λem 488 nm (only DAPI). Excitation: λex 360nm, Emission: λem 460nm (DAPI-DNA complex).
- Smiles
- NC(C1=CC=C2C(NC(C3=CC=C(C(N)=N)C=C3)=C2)=C1)=N.Cl.Cl
- InChi Key
- FPNZBYLXNYPRLR-UHFFFAOYSA-N
- References
- (1) W.C. Russell, et al., Nature 253, 461 (1975) , (2) I.W. Taylor & B.K. Milthorpe, J. Histochem. Cytochem. 28, 1224 (1980) , (3) F. Otto & K.C. Tsou, Stain Technol. 60, 7 (1985) , (4) C. Cubria, et al., Comp. Biochem. Physiol. C. 105, 251 (1994) , (5) M.A. Hotz, et al., Cytometry 15, 237 (1994) , (6) J. Kapuscinski, Biotech. Histochem. 70, 220 (1995) , (7) E. Buel & M. Schwartz, J. Forensic Sci. 40, 275 (1995) , (8) D. Zink, et al., Methods: A Companion to Methods in Enzymology 29, 42 (2003) , (9) L. Rodgers, CSH Protoc. 2006, pdb.prot4438 (2006) , (10) B. Chazotte, CSH Protoc. 2011, pdb.prot5556 (2011) , (11) A. Biancardi, et al., Phys. Chem. Chem. Phys. 15, 4596 (2013) , (12) M. Jez, et al., Histochem. Cell Biol. 139, 195 (2013) , (13) G. Yahav, et al., Cytometry A 89, 644 (2016)
DAPI (4?,6-Diamidino-2-phenylindole dihydrochloride) is a cell permeable, fluorescent dye that is a minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone. DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma. Spectral Data: Excitation: λex 340 nm, Emission: λem 488 nm (only DAPI). Excitation: λex 360nm, Emission: λem 460nm (DAPI-DNA complex).
Additional information
| Synonyms | 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride, 4',6-Diamidino-2-phenylindole dihydrochloride, FxCycle Violet |
|---|---|
| Purity | ≥95% (HPLC) |
| Appearance | Yellow powder |
| CAS-Number | 28718-90-3 |
| Molecular Formula | C16H15N5 · 2HCl |
| Molecular Weight | 350.25 |
| Identity | 1H-NMR |
| Solvents | water (10 mg/ml,warming), DMSO, methanol or ethanol (slightly) |
| Melting Point | 330°C (dec.) |
| Other | pH: 4.0-5.0 at 10 g/l at 20°C |
| Smiles | NC(C1=CC=C2C(NC(C3=CC=C(C(N)=N)C=C3)=C2)=C1)=N.Cl.Cl |
| InChi Key | FPNZBYLXNYPRLR-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Hazard statements | H315, H319, H335 |
| Precautionary statements | P261, P271, P280 |
| GHS Symbol | GHS07 |
| Transportation | Not dangerous goods |
| References | (1) W.C. Russell, et al., Nature 253, 461 (1975), (2) I.W. Taylor & B.K. Milthorpe, J. Histochem. Cytochem. 28, 1224 (1980), (3) F. Otto & K.C. Tsou, Stain Technol. 60, 7 (1985), (4) C. Cubria, et al., Comp. Biochem. Physiol. C. 105, 251 (1994), (5) M.A. Hotz, et al., Cytometry 15, 237 (1994), (6) J. Kapuscinski, Biotech. Histochem. 70, 220 (1995), (7) E. Buel & M. Schwartz, J. Forensic Sci. 40, 275 (1995), (8) D. Zink, et al., Methods: A Companion to Methods in Enzymology 29, 42 (2003), (9) L. Rodgers, CSH Protoc. 2006, pdb.prot4438 (2006), (10) B. Chazotte, CSH Protoc. 2011, pdb.prot5556 (2011), (11) A. Biancardi, et al., Phys. Chem. Chem. Phys. 15, 4596 (2013), (12) M. Jez, et al., Histochem. Cell Biol. 139, 195 (2013), (13) G. Yahav, et al., Cytometry A 89, 644 (2016) |
| Quantity | 10 mg, 50 mg, Bulk |
