DCFDA

Available from stock

DCFDA

SKU
D0063

Categories: ,

Synonyms
2',7'-Dichlorofluorescein diacetate , Diacetyldichlorofluorescein
2044-85-1
CAS-Number
C24H14Cl2O7
Molecular Formula
485.27
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Specifications

Purity
≥95% (HPLC)
Appearance
White powder
Identity
1H-NMR
Residual Solvent
Contains ≤ 10% of acetic acid

Properties

Solvents
DMSO, acetone
Melting Point
232-234 °C (lit.)
Fluorescence
λex 504 nm, λem 524 nm in 0.1 M Tris pH 8.0 (esterase)

Documentation

Safety Data Sheet (SDS)
CDX D0063 MSDS.pdf
Shipping
AMBIENT
Short Term Storage
RT
Long Term Storage
+4°C
Handling Advice
Very hygroscopic. Store under inert gas.
Use / Stability
Stable for at least 2 years after receipt when stored at +4°C.
RIDADR
UN3263
Transportation
Not dangerous goods
Description
A cell-permeable fluorogenic dye useful for the detection of reactive oxygen species (ROS) and nitric oxide (NO) and for the determination of the degree of overall oxidative stress. The fluorescence of DCFDA indicates oxidation by hydrogen peroxide, peroxynitrite or hydroxyl radical. Superoxide anions can also contribute to DCFDA oxidation albeit at a lesser degree. It measures hydroxyl, peroxyl and other reactive oxygen species (ROS) activity within the cell. After diffusion in to the cell, DCFDA is deacetylated by cellular esterases to a non-fluorescent compound, which is later oxidized by ROS into the highly fluorescent 2’,7’-dichlorofluorescein (DCF). Spectral data: λex=504nm, λem=524nm in 0.1 M Tris pH 8.0 (esterase).
Smiles
CC(OC1=C(Cl)C=C2C(OC3=CC(OC(C)=O)=C(Cl)C=C3C24OC(C5=C4C=CC=C5)=O)=C1)=O
InChi Key
VQVUBYASAICPFU-UHFFFAOYSA-N
References
(1) A.R. Rosenkranz, et al., J. Immunol. Meth. 156, 39 (1992) , (2) T. Takanashi, et al., Invest. Ophthalmol. Vis. Sci. 38, 2721 (1997) , (3) E. Eruslanov & S. Kusmartsev, Methods Mol. Biol. 594, 57 (2010)
InChi
InChI=1S/C24H14Cl2O7/c1-11(27)30-21-9-19-15(7-17(21)25)24(14-6-4-3-5-13(14)23(29)33-24)16-8-18(26)22(31-12(2)28)10-20(16)32-19/h3-10H,1-2H3

A cell-permeable fluorogenic dye useful for the detection of reactive oxygen species (ROS) and nitric oxide (NO) and for the determination of the degree of overall oxidative stress. The fluorescence of DCFDA indicates oxidation by hydrogen peroxide, peroxynitrite or hydroxyl radical. Superoxide anions can also contribute to DCFDA oxidation albeit at a lesser degree. It measures hydroxyl, peroxyl and other reactive oxygen species (ROS) activity within the cell. After diffusion in to the cell, DCFDA is deacetylated by cellular esterases to a non-fluorescent compound, which is later oxidized by ROS into the highly fluorescent 2’,7’-dichlorofluorescein (DCF). Spectral data: λex=504nm, λem=524nm in 0.1 M Tris pH 8.0 (esterase).

SKU: D0063 Categories: ,

Additional information

Synonyms

2',7'-Dichlorofluorescein diacetate, Diacetyldichlorofluorescein
Purity

≥95% (HPLC)
Appearance

White powder
CAS-Number

2044-85-1
Molecular Formula

C24H14Cl2O7
Molecular Weight

485.27
Identity

1H-NMR
Solvents

DMSO, acetone
Melting Point

232-234 °C (lit.)
Fluorescence

λex 504 nm, λem 524 nm in 0.1 M Tris pH 8.0 (esterase)
Smiles

CC(OC1=C(Cl)C=C2C(OC3=CC(OC(C)=O)=C(Cl)C=C3C24OC(C5=C4C=CC=C5)=O)=C1)=O
InChi Key

VQVUBYASAICPFU-UHFFFAOYSA-N
Shipping

AMBIENT
Short Term Storage

RT
Long Term Storage

+4°C
Handling Advice

Very hygroscopic. Store under inert gas.
Use / Stability

Stable for at least 2 years after receipt when stored at +4°C.
RIDADR

UN3263
Transportation

Not dangerous goods
References

(1) A.R. Rosenkranz, et al., J. Immunol. Meth. 156, 39 (1992), (2) T. Takanashi, et al., Invest. Ophthalmol. Vis. Sci. 38, 2721 (1997), (3) E. Eruslanov & S. Kusmartsev, Methods Mol. Biol. 594, 57 (2010)
InChi

InChI=1S/C24H14Cl2O7/c1-11(27)30-21-9-19-15(7-17(21)25)24(14-6-4-3-5-13(14)23(29)33-24)16-8-18(26)22(31-12(2)28)10-20(16)32-19/h3-10H,1-2H3
Quantity

1 g, 5 g, Bulk
cdx-Residual Solvent

Contains ≤ 10% of acetic acid

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