Specifications
- Purity
- ≥98% (NMR)
- Appearance
- Faint to dark orange powder
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO (10 mg/ml), DMF (20 mg/ml)
Available from stock
Dibenzylfluorescein
- SKU
- D0282
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- DBF , NSC 645658
- 97744-44-0
- CAS-Number
- C34H24O5
- Molecular Formula
- 512.55
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- -20°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Hazard statements
- H302-H312-H332
- Precautionary statements
- P280
- GHS Symbol
- GHS07
- Signal word
- Warning
- Transportation
- Not dangerous goods
- Description
- Dibenzylfluorescein is a fluorogenic probe that acts as a substrate for specific cytochrome P450 (CYP) isoforms, including CYP3A4, CYP2C8, CYP2C9, CYP2C19 and aromatase (CYP19). It is dealkylated by these CYP isoforms to produce fluorescein benzyl ether, which is further hydrolyzed to fluorescein by the addition of base (typically 2 M NaOH). Dibenzylfluorescein is typically used near its apparent Km value of 0.87-1.9 µM. The fluorescence of fluorescein is evaluated using excitation/emission wavelengths of 485/538nm. Dibenzylfluorescein is used to detect changes in CYP catalytic activity caused by drugs or disease.
- Smiles
- O=C(OCC1=CC=CC=C1)C2=CC=CC=C2C(C3=CC=C(OCC4=CC=CC=C4)C=C3O5)=C6C5=CC(C=C6)=O
- InChi Key
- YZJGKSLPSGPFEV-UHFFFAOYSA-N
- References
- (1) D.M. Stresser, et al., Drug Metab. Dispos. 28, 1440 (2000) , (2) M.T. Donato, et al., Drug Metab. Dispos. 32, 699 (2004) , (3) E.R. Troesken, et al., Toxicol. 219, 33 (2006) , (4) K.A. Salminen, et al., Drug Metab. Dispos. 39, 412 (2011) , (5) D. Moutinho, et al., Drug Metab. Dispos. 40, 754 (2012)
- InChi
- InChI=1S/C34H24O5/c35-25-15-17-29-31(19-25)39-32-20-26(37-21-23-9-3-1-4-10-23)16-18-30(32)33(29)27-13-7-8-14-28(27)34(36)38-22-24-11-5-2-6-12-24/h1-20H,21-22H2
Dibenzylfluorescein is a fluorogenic probe that acts as a substrate for specific cytochrome P450 (CYP) isoforms, including CYP3A4, CYP2C8, CYP2C9, CYP2C19 and aromatase (CYP19). It is dealkylated by these CYP isoforms to produce fluorescein benzyl ether, which is further hydrolyzed to fluorescein by the addition of base (typically 2 M NaOH). Dibenzylfluorescein is typically used near its apparent Km value of 0.87-1.9 µM. The fluorescence of fluorescein is evaluated using excitation/emission wavelengths of 485/538nm. Dibenzylfluorescein is used to detect changes in CYP catalytic activity caused by drugs or disease.
Additional information
| Synonyms | DBF, NSC 645658 |
|---|---|
| Purity | ≥98% (NMR) |
| Appearance | Faint to dark orange powder |
| CAS-Number | 97744-44-0 |
| Molecular Formula | C34H24O5 |
| Molecular Weight | 512.55 |
| Identity | 1H-NMR |
| Solvents | DMSO (10 mg/ml), DMF (20 mg/ml) |
| Smiles | O=C(OCC1=CC=CC=C1)C2=CC=CC=C2C(C3=CC=C(OCC4=CC=CC=C4)C=C3O5)=C6C5=CC(C=C6)=O |
| InChi Key | YZJGKSLPSGPFEV-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | -20°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Hazard statements | H302-H312-H332 |
| Precautionary statements | P280 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) D.M. Stresser, et al., Drug Metab. Dispos. 28, 1440 (2000), (2) M.T. Donato, et al., Drug Metab. Dispos. 32, 699 (2004), (3) E.R. Troesken, et al., Toxicol. 219, 33 (2006), (4) K.A. Salminen, et al., Drug Metab. Dispos. 39, 412 (2011), (5) D. Moutinho, et al., Drug Metab. Dispos. 40, 754 (2012) |
| InChi | InChI=1S/C34H24O5/c35-25-15-17-29-31(19-25)39-32-20-26(37-21-23-9-3-1-4-10-23)16-18-30(32)33(29)27-13-7-8-14-28(27)34(36)38-22-24-11-5-2-6-12-24/h1-20H,21-22H2 |
| Quantity | 100 mg, 500 mg, Bulk |
