Available from stock

Dihydrofluorescein diacetate

SKU: D0122

Categories: Fluorescent Detection, Fluorescent Detection

Synonyms: Diacetyldihydrofluorescein; 35340-49-9
CAS-Number: C₂₄H₁₈O₇
Molecular Formula: 418.4
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥97% (NMR)
Appearance: Yellow powder
Identity: 1H-NMR

Properties

Solvents: chloroform
Melting Point: 212-215 °C
Fluorescence: λex 272 nm in methanol, λex 490, λem 514 (Reaction product)

Documentation

Safety Data Sheet (SDS): CDX D0122 MSDS.pdf

Shipping: AMBIENT
Short Term Storage: +4°C
Long Term Storage: -20°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.

Transportation: Not dangerous goods

Description: Dihydrofluorescein diacetate is a fluorescent probe for detecting intracellular oxidants. It is hydrolyzed by cellular esterases to dihydrofluorescein and is then oxidized to fluorescein primarily by H2O2. Dihydrofluorescein diacetate might be reactive toward a broad range of oxidizing reactions that may be increased during intracellular oxidant stress. Cell-loading studies indicate that dihydrofluorescein achieves higher intracellular concentrations than the other redox sensors such as Dihydrorhodamine 123 (Ex/Em: 490/514nm).
Smiles: CC(=O)OC1=CC2=C(C=C1)C(C1=C(O2)C=C(OC(C)=O)C=C1)C1=C(C=CC=C1)C(O)=O
InChi Key: YKSJJXGQHSESKB-UHFFFAOYSA-N
References: (1) P.P. Williams & R.L. Stolzenberg: Applied Microbiology 23(4), 745-9 (1972) , (2) S.L. Hempel, et al.: Free Radical Biology & Medicine 27(1/2), 146-159 (1999) , (3) Y. O'Malley et al.: Free Radical Biology & Medicine 36(1), 90-100 (2004)
InChi: InChI=1S/C24H18O7/c1-13(25)29-15-7-9-19-21(11-15)31-22-12-16(30-14(2)26)8-10-20(22)23(19)17-5-3-4-6-18(17)24(27)28/h3-12,23H,1-2H3,(H,27,28)

Dihydrofluorescein diacetate is a fluorescent probe for detecting intracellular oxidants. It is hydrolyzed by cellular esterases to dihydrofluorescein and is then oxidized to fluorescein primarily by H2O2. Dihydrofluorescein diacetate might be reactive toward a broad range of oxidizing reactions that may be increased during intracellular oxidant stress. Cell-loading studies indicate that dihydrofluorescein achieves higher intracellular concentrations than the other redox sensors such as Dihydrorhodamine 123 (Ex/Em: 490/514nm).

SKU: D0122 Categories: Fluorescent Detection, Fluorescent Detection

Additional information

Additional information

Synonyms	Diacetyldihydrofluorescein
Purity	≥97% (NMR)
Appearance	Yellow powder
CAS-Number	35340-49-9
Molecular Formula	C24H18O7
Molecular Weight	418.4
Identity	1H-NMR
Solvents	chloroform
Melting Point	212-215 °C
Fluorescence	λex 272 nm in methanol, λex 490, λem 514 (Reaction product)
Smiles	CC(=O)OC1=CC2=C(C=C1)C(C1=C(O2)C=C(OC(C)=O)C=C1)C1=C(C=CC=C1)C(O)=O
InChi Key	YKSJJXGQHSESKB-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Transportation	Not dangerous goods
References	(1) P.P. Williams & R.L. Stolzenberg: Applied Microbiology 23(4), 745-9 (1972), (2) S.L. Hempel, et al.: Free Radical Biology & Medicine 27(1/2), 146-159 (1999), (3) Y. O'Malley et al.: Free Radical Biology & Medicine 36(1), 90-100 (2004)
InChi	InChI=1S/C24H18O7/c1-13(25)29-15-7-9-19-21(11-15)31-22-12-16(30-14(2)26)8-10-20(22)23(19)17-5-3-4-6-18(17)24(27)28/h3-12,23H,1-2H3,(H,27,28)
Quantity	1 g, 10 g, Bulk