Dihydrorhodamine 123

Specifications

Purity

≥95% (NMR)

Identity

1H-NMR

Appearance

Pink solid

Properties

Fluorescence

λex 500 nm, λem 525 nm in 0.1 M Tris pH 8.0 (after oxidizing with H2O2, peroxidase)

Solvents

DMSO (5 mg/ml), DMF, chloroform, ethanol

Short Term Storage

+4°C

Use / Stability

Stable for at least 2 years after receipt when stored at -20°C.

Handling Advice

Protect from light and moisture.

Long Term Storage

-20°C

Shipping

AMBIENT

Transportation

Not dangerous goods

Description

Cell-permeable non-fluorescent reactive oxygen species (ROS) indicator. Passively diffuse across membranes where it is oxidized by peroxynitrite to rhodamine 123 which localizes in the mitochondria and exhibits green fluorescence (Ex/Em wavelengths of 500 and 536 nm). Used to investigate reactive oxygen intermediates produced by human and murine phagocytes, activated rat mast cells and cultured endothelial cells. In addition, it has been used together with Fura Red calcium indicator to simultaneously measure oxidative bursts and Ca2+ fluxes in monocytes and granulocytes. Dihydrorhodamine 123 has been shown to be a more sensitive probe than H2DCFDA for detecting granulocyte respiratory bursts.

InChi

InChI=1S/C21H18N2O3/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20/h2-11,20H,22-23H2,1H3

References

(1) K. Hensley et al.: Meth. Biol. Oxidative Stress 169 (2003) , (2) H. Ischiropoulos et al.: Meth. Enzymology 301, 367 (1999) , (3) L.M. Henderson et al.: Europ. J. Biochem. FEBS 217(3), 973 (1993)

InChi Key

FNEZBBILNYNQGC-UHFFFAOYSA-N

Smiles

COC(=O)C1=CC=CC=C1C1C2=CC=C(N)C=C2OC2=C1C=CC(N)=C2