Available from stock

Ethidium homodimer

SKU: E0012

Categories: Fluorescent Detection, Fluorescent Detection

Synonyms: EthD-1 , EtDi; 61926-22-5
CAS-Number: C₄₆H₅₀Cl₄N₈
Molecular Formula: 856.75
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ~90% (HPCE)
Appearance: Dark red powder
Identity: 1H-NMR

Properties

Solvents: water, DMSO or DMF
Melting Point: ≥250 °C
Fluorescence: λex 528 nm, λem 617 nm in TE buffer, DANN

Documentation

Safety Data Sheet (SDS): CDX E0012 MSDS.pdf

Shipping: AMBIENT
Short Term Storage: +4°C
Long Term Storage: -20°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.

Hazard statements: H261, H314, H351
Precautionary statements: P231 + P232, P280, P305 + P351 + P338, P310, P422
GHS Symbol: GHS02+GHS05+GHS08
Signal word: Danger
RIDADR: UN3096 8 (4.3)
Transportation: Excepted Quantities

Description: Staining dye for ssDNA, dsDNA, RNA, oligonucleotides and triplex DNA. It does not cross intact cell membranes and can be used to test cell viability. Reagent for the fluorimetric detection of nucleic acids.
Smiles: [Cl-].[Cl-].NC1=CC2=C(C3=CC=CC=C3)[N+](CCCNCCNCCC[N+]3=C(C4=CC=CC=C4)C4=C(C=CC(N)=C4)C4=C3C=C(N)C=C4)=C3C=C(N)C=CC3=C2C=C1
InChi Key: BGWLYQZDNFIFRX-UHFFFAOYSA-N
References: (1) A.C. Barti et al.: J. Biol. Chem. 279(7), 6065 (2004) , (2) E.M. Talavera et al.: Applied Spectr. 57(2), 208 (2003) , (3) E. Tuite et al.: Bioorg. Med. Chem. 3, 701 (1995) , (4) J. Markovits et al: Anal. Biochem. 94, 259 (1979)
InChi: InChI=1S/C46H46N8.2ClH/c47-33-13-17-37-39-19-15-35(49)29-43(39)53(45(41(37)27-33)31-9-3-1-4-10-31)25-7-21-51-23-24-52-22-8-26-54-44-30-36(50)16-20-40(44)38-18-14-34(48)28-42(38)46(54)32-11-5-2-6-12-32

Staining dye for ssDNA, dsDNA, RNA, oligonucleotides and triplex DNA. It does not cross intact cell membranes and can be used to test cell viability. Reagent for the fluorimetric detection of nucleic acids.

SKU: E0012 Categories: Fluorescent Detection, Fluorescent Detection

Additional information

Additional information

Synonyms	EthD-1, EtDi
Purity	~90% (HPCE)
Appearance	Dark red powder
CAS-Number	61926-22-5
Molecular Formula	C46H50Cl4N8
Molecular Weight	856.75
Identity	1H-NMR
Solvents	water, DMSO or DMF
Melting Point	≥250 °C
Fluorescence	λex 528 nm, λem 617 nm in TE buffer, DANN
Smiles	[Cl-].[Cl-].NC1=CC2=C(C3=CC=CC=C3)[N+](CCCNCCNCCC[N+]3=C(C4=CC=CC=C4)C4=C(C=CC(N)=C4)C4=C3C=C(N)C=C4)=C3C=C(N)C=CC3=C2C=C1
InChi Key	BGWLYQZDNFIFRX-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Hazard statements	H261, H314, H351
Precautionary statements	P231 + P232, P280, P305 + P351 + P338, P310, P422
GHS Symbol	GHS02+GHS05+GHS08
Signal word	Danger
RIDADR	UN3096 8 (4.3)
Transportation	Excepted Quantities
References	(1) A.C. Barti et al.: J. Biol. Chem. 279(7), 6065 (2004), (2) E.M. Talavera et al.: Applied Spectr. 57(2), 208 (2003), (3) E. Tuite et al.: Bioorg. Med. Chem. 3, 701 (1995), (4) J. Markovits et al: Anal. Biochem. 94, 259 (1979)
InChi	InChI=1S/C46H46N8.2ClH/c47-33-13-17-37-39-19-15-35(49)29-43(39)53(45(41(37)27-33)31-9-3-1-4-10-31)25-7-21-51-23-24-52-22-8-26-54-44-30-36(50)16-20-40(44)38-18-14-34(48)28-42(38)46(54)32-11-5-2-6-12-32
Quantity	1 mg, 5 mg, 10 mg, Bulk