Specifications
- Purity
- ≥90% (TLC)
- Appearance
- Dark red powder
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO
- Fluorescence
- λex 506 nm, λem 526 nm in 0.1 M Tris pH 8.0, esterase, 30 mM Ca2+
Available from stock
Fluo-3 AM
- SKU
- F0033
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 4-(6-Acetoxymethoxy-2,7-dichloro-3-oxo-9-xanthenyl)-4'-methyl-2,2'(ethylenedioxy)dianiline-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl) ester
- 121714-22-5
- CAS-Number
- C51H50Cl2N2O23
- Molecular Formula
- 1129.85
- Molecular Weight
- Shipping
- BLUE ICE
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Transportation
- Not dangerous goods
- Description
- Ca2+-indicator fluo-3 for use with visible-light excitation sources in flow cytometry and confocal laser-scanning microscopy. Since about 1996, fluo-3 has also been extensively used in cell-based high-throughput screening assays for drug discovery. Fluo-3 AM is essentially nonfluorescent until it is hydrolyzed intracellularly and/or in the presence of Ca2+. The green-fluorescent emission (~525 nm) of Ca2+-bound fluo-3 is conventionally detected using optical filter sets designed for fluorescein (FITC). As prepared by Minta, Kao and Tsien, fluo-3 was originally reported to undergo an ~40-fold increase in fluorescence upon binding Ca2+.
- Smiles
- CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)C1=CC=C(C=C1OCCOC1=C(C=CC(C)=C1)N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)C1=C2C=C(Cl)C(=O)C=C2OC2=CC(OCOC(C)=O)=C(Cl)C=C12
- InChi Key
- PTPUOMXKXCCSEN-UHFFFAOYSA-N
- References
- (1) T. Toyofuku et al.: Geochem. Geophys. Geosyst. 9(5) , (2008) , (2) D.M. Plank et al.: Meth. Cell Sc. 25(3-4), 123 (2004) , (3) P. Vandenberghe et al.: J. Immun.Meth. 127, 197 (1990) , (4) J.P.Y. Kao et al.: J. Biol. Chem. 264, 8179 (1989)
- InChi
- InChI=1S/C51H50Cl2N2O23/c1-28-7-9-39(54(19-47(62)74-24-69-30(3)57)20-48(63)75-25-70-31(4)58)45(13-28)66-11-12-67-46-14-34(8-10-40(46)55(21-49(64)76-26-71-32(5)59)22-50(65)77-27-72-33(6)60)51-35-15-37(52)41(61)17-42(35)78-43-18-44(38(53)16-36(43)51)73-23-68-29(2)56/h7-10,13-18H,11-12,19-27H2,1-6H3
Ca2+-indicator fluo-3 for use with visible-light excitation sources in flow cytometry and confocal laser-scanning microscopy. Since about 1996, fluo-3 has also been extensively used in cell-based high-throughput screening assays for drug discovery. Fluo-3 AM is essentially nonfluorescent until it is hydrolyzed intracellularly and/or in the presence of Ca2+. The green-fluorescent emission (~525 nm) of Ca2+-bound fluo-3 is conventionally detected using optical filter sets designed for fluorescein (FITC). As prepared by Minta, Kao and Tsien, fluo-3 was originally reported to undergo an ~40-fold increase in fluorescence upon binding Ca2+.
Additional information
| Synonyms | 4-(6-Acetoxymethoxy-2,7-dichloro-3-oxo-9-xanthenyl)-4'-methyl-2,2'(ethylenedioxy)dianiline-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl) ester |
|---|---|
| Purity | ≥90% (TLC) |
| Appearance | Dark red powder |
| CAS-Number | 121714-22-5 |
| Molecular Formula | C51H50Cl2N2O23 |
| Molecular Weight | 1129.85 |
| Identity | 1H-NMR |
| Solvents | DMSO |
| Fluorescence | λex 506 nm, λem 526 nm in 0.1 M Tris pH 8.0, esterase, 30 mM Ca2+ |
| Smiles | CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)C1=CC=C(C=C1OCCOC1=C(C=CC(C)=C1)N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)C1=C2C=C(Cl)C(=O)C=C2OC2=CC(OCOC(C)=O)=C(Cl)C=C12 |
| InChi Key | PTPUOMXKXCCSEN-UHFFFAOYSA-N |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Transportation | Not dangerous goods |
| References | (1) T. Toyofuku et al.: Geochem. Geophys. Geosyst. 9(5), (2008), (2) D.M. Plank et al.: Meth. Cell Sc. 25(3-4), 123 (2004), (3) P. Vandenberghe et al.: J. Immun.Meth. 127, 197 (1990), (4) J.P.Y. Kao et al.: J. Biol. Chem. 264, 8179 (1989) |
| InChi | InChI=1S/C51H50Cl2N2O23/c1-28-7-9-39(54(19-47(62)74-24-69-30(3)57)20-48(63)75-25-70-31(4)58)45(13-28)66-11-12-67-46-14-34(8-10-40(46)55(21-49(64)76-26-71-32(5)59)22-50(65)77-27-72-33(6)60)51-35-15-37(52)41(61)17-42(35)78-43-18-44(38(53)16-36(43)51)73-23-68-29(2)56/h7-10,13-18H,11-12,19-27H2,1-6H3 |
| Quantity | 1 mg, 10 mg, Bulk |
