Specifications
- Purity
- ~70%
- Appearance
- Dark crystalline solid
- Identity
- 1H-NMR
Properties
- Solvents
- water
- Boiling Point
- 1056 °C at 760 mmHg
- UV / Vis
- λex 506 nm, λem 526 nm in 0.1 M Tris pH 8.0, esterase, 30 mM Ca2+
- Density
- 1.66 g/cm3
Available from stock
Fluo-3
- SKU
- F0056
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 4-(2,7-Dichloro-6-hydroxy-3-oxo-9-xanthenyl)-4'-methyl-2,2'-(ethylenedioxy)dianiline-N,N,N'N'-tetraacetic acid
- 123632-39-3
- CAS-Number
- C36H30Cl2N2O13
- Molecular Formula
- 769.53
- Molecular Weight
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Transportation
- Not dangerous goods
- Description
- Fluorescent indicator for calcium used in flow cytometry. Fluorescence intensity increases about 40-fold after calcium binding.
- Smiles
- CC1=CC(=C(C=C1)N(CC(=O)O)CC(=O)O)OCCOC2=C(C=CC(=C2)C3=C4C=C(C(=O)C=C4OC5=CC(=C(C=C53)Cl)O)Cl)N(CC(=O)O)CC(=O)O
- InChi Key
- OZLGRUXZXMRXGP-VXANVLNBCJ
- References
- (1) P.A. Vandenberghe, J.L. Ceuppens: J. Immunol. Methods 127, 197 (1990) , (2) A. Minta et al.: J. Biol. Chem. 264, 8171 (1989)
- InChi
- InChI=1/C36H30Cl2N2O13/c1-18-2-4-24(39(14-32(43)44)15-33(45)46)30(8-18)51-6-7-52-31-9-19(3-5-25(31)40(16-34(47)48)17-35(49)50)36-20-10-22(37)26(41)12-28(20)53-29-13-27(42)23(38)11-21(29)36/h2-5,8-13,41H,6-7,14-17H2,1H3,(H,43,44)(H,45,46)(H,47,48)(H,49,50)
Fluorescent indicator for calcium used in flow cytometry. Fluorescence intensity increases about 40-fold after calcium binding.
Additional information
| Synonyms | 4-(2,7-Dichloro-6-hydroxy-3-oxo-9-xanthenyl)-4'-methyl-2,2'-(ethylenedioxy)dianiline-N,N,N'N'-tetraacetic acid |
|---|---|
| Purity | ~70% |
| Appearance | Dark crystalline solid |
| CAS-Number | 123632-39-3 |
| Molecular Formula | C36H30Cl2N2O13 |
| Molecular Weight | 769.53 |
| Identity | 1H-NMR |
| Solvents | water |
| Boiling Point | 1056 °C at 760 mmHg |
| UV / Vis | λex 506 nm, λem 526 nm in 0.1 M Tris pH 8.0, esterase, 30 mM Ca2+ |
| Density | 1.66 g/cm3 |
| Smiles | CC1=CC(=C(C=C1)N(CC(=O)O)CC(=O)O)OCCOC2=C(C=CC(=C2)C3=C4C=C(C(=O)C=C4OC5=CC(=C(C=C53)Cl)O)Cl)N(CC(=O)O)CC(=O)O |
| InChi Key | OZLGRUXZXMRXGP-VXANVLNBCJ |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Transportation | Not dangerous goods |
| References | (1) P.A. Vandenberghe, J.L. Ceuppens: J. Immunol. Methods 127, 197 (1990), (2) A. Minta et al.: J. Biol. Chem. 264, 8171 (1989) |
| InChi | InChI=1/C36H30Cl2N2O13/c1-18-2-4-24(39(14-32(43)44)15-33(45)46)30(8-18)51-6-7-52-31-9-19(3-5-25(31)40(16-34(47)48)17-35(49)50)36-20-10-22(37)26(41)12-28(20)53-29-13-27(42)23(38)11-21(29)36/h2-5,8-13,41H,6-7,14-17H2,1H3,(H,43,44)(H,45,46)(H,47,48)(H,49,50) |
| Quantity | 1 mg, 5 mg, Bulk |
