Fluorescein-5-thiosemicarbazide

Category: Fluorescent Detection

Synonyms: N-(3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)-hydrazinecarbothioamide; 76863-28-0
CAS-Number: C₂₁H₁₅N₃O₅S
Molecular Formula: 421.43
Molecular Weight

Short Term Storage: +4°C
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.
Handling Advice: Protect from light and moisture.
Long Term Storage: -20°C
Shipping: AMBIENT

Description: Labeling of cell-surface functional groups (glycophorins). Detection of protein carbonyls in aging tissues.
InChi: InChI=1S/C21H15N3O5S/c22-24-20(30)23-10-1-4-14-13(7-10)19(27)29-21(14)15-5-2-11(25)8-17(15)28-18-9-12(26)3-6-16(18)21/h1-9,25-26H,22H2,(H2,23,24,30)
References: (1) A.R. Chauduri et al.: Mech. Ageing Developm. 127(11), 849 (2006) , (2) X. Gao et al.: Bioorg.Chem. 25(3), 163 (1997) , (3) J.D. Corbett et al.: Biophys. J. 66, 25 (1994)
InChi Key: OIMMDIXLTJAVTF-UHFFFAOYSA-N
Smiles: NNC(=S)NC1=CC2=C(C=C1)C1(OC2=O)C2=C(OC3=C1C=CC(O)=C3)C=C(O)C=C2

Labeling of cell-surface functional groups (glycophorins). Detection of protein carbonyls in aging tissues.

SKU: F0005 Category: Fluorescent Detection

Additional information

Synonyms	N-(3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)-hydrazinecarbothioamide
Purity	≥97% (NMR)
Appearance	Orange to brown powder
CAS-Number	76863-28-0
Molecular Formula	C21H15N3O5S
Molecular Weight	421.43
Identity	1H-NMR
Solvents	water, DMF, DMSO
Fluorescence	λex 492 nm, λem 516 nm in 0.1 M Tris pH 9.0
Smiles	NNC(=S)NC1=CC2=C(C=C1)C1(OC2=O)C2=C(OC3=C1C=CC(O)=C3)C=C(O)C=C2
InChi Key	OIMMDIXLTJAVTF-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Transportation	Not dangerous goods
References	(1) A.R. Chauduri et al.: Mech. Ageing Developm. 127(11), 849 (2006), (2) X. Gao et al.: Bioorg.Chem. 25(3), 163 (1997), (3) J.D. Corbett et al.: Biophys. J. 66, 25 (1994)
InChi	InChI=1S/C21H15N3O5S/c22-24-20(30)23-10-1-4-14-13(7-10)19(27)29-21(14)15-5-2-11(25)8-17(15)28-18-9-12(26)3-6-16(18)21/h1-9,25-26H,22H2,(H2,23,24,30)
Quantity	50 mg, 100 mg, 500 mg, Bulk