Specifications
- Purity
- ≥97% (NMR)
- Appearance
- Faint yellow powder
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO or chloroform.
- Melting Point
- 82-83 °C
Category: Fluorescent Detection, Fluorescent Detection
Fluorescein dicaproate is a non-fluorescent hydrophobic fluorescein derivative that can pass through the cell membrane whereupon intracellular esterases hydrolyze the dicaproate group producing the highly fluorescent product fluorescein. The fluorescein molecules accumulate in cells. Fluorescein dicaproate is a fluorogenic substrate for C6 esterase that generates green fluorescence upon esterase cleavage. Spectral Data: λEx 497nM, &lamda,Em 516nm. Extinction coefficient: 80000 cm-1 M-1.
Synonyms | Fluorescein dihexanoate |
---|---|
Purity | ≥97% (NMR) |
Appearance | Faint yellow powder |
CAS-Number | 7364-90-1 |
Molecular Formula | C32H32O7 |
Molecular Weight | 528.59 |
Identity | 1H-NMR |
Solvents | DMSO or chloroform. |
Melting Point | 82-83 °C |
Smiles | O=C(O1)C2=C(C=CC=C2)C31C4=C(C=C(OC(CCCCC)=O)C=C4)OC5=CC(OC(CCCCC)=O)=CC=C53 |
InChi Key | DHSNCRHSIFNRJY-UHFFFAOYSA-N |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
Transportation | Not dangerous goods |
References | (1) B. Rotman & B.W. Papermaster, PNAS 55, 134 (1966), (2) F.-Y. Ge, et al., Dyes Pigm. 72, 322 (2007) |
InChi | InChI=1S/C32H32O7/c1-3-5-7-13-29(33)36-21-15-17-25-27(19-21)38-28-20-22(37-30(34)14-8-6-4-2)16-18-26(28)32(25)24-12-10-9-11-23(24)31(35)39-32/h9-12,15-20H,3-8,13-14H2,1-2H3 |
Quantity | 1 g, Bulk |