Specifications
- Purity
- ≥98% (NMR)
- Appearance
- Off-white low melting solid
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO (5 mg/ml)
- Melting Point
- 49 °C
Available from stock
Fluorescein dioctanoate
- SKU
- F0087
Category: Non Fluorescent Detection & Chromogene Detection
- Synonyms
- FDO , Fluorescein dicaprylate , 3-Oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3',6'-diyl ester octanoic acid
- 19722-86-2
- CAS-Number
- C36H40O7
- Molecular Formula
- 584.72
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- +4°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at +4°C.
- Transportation
- Not dangerous goods
- Description
- Fluorescein dioctanoate is a non-fluorescent hydrophobic fluorescein derivative that can pass through the cell membrane whereupon intracellular esterases hydrolyze the dioctanoate group producing the highly fluorescent product fluorescein. The fluorescein molecules accumulate in cells that possess intact membranes so the green fluorescence can be used as a marker of cell viability. Cells that do not possess an intact cell membrane or an active metabolism may not accumulate the fluorescent product and therefore do not exhibit green fluorescence. Fluorescein dioctanoate may be used in combination with PI staining as the non-viable cells take up the PI and stain dead cells red whereas viable cells do not take up the PI and should only stain green. Fluorescein dioctanoate is a fluorogenic substrate for C8 esterase that generates green fluorescence upon esterase cleavage. Spectral Data: &lambda,Ex 497nM, &lamda,Em 516nm. Extinction coefficient: 80000 cm-1 M-1.
- Smiles
- O=C(O1)C2=C(C=CC=C2)C31C4=CC=C(OC(CCCCCCC)=O)C=C4OC5=C3C=CC(OC(CCCCCCC)=O)=C5
- InChi Key
- UWZNXQHGTKMTSX-UHFFFAOYSA-N
- References
- (1) J.J. Thomas, et al., Exp. Biol. Med. 140, 179 (1972) , (2) F.-Y. Ge, et al., Dyes Pigm. 72, 322 (2007) , (3) M. Poznika & B. Koeniga, Org. Biomol. Chem. 12, 3175 (2014)
- InChi
- InChI=1S/C36H40O7/c1-3-5-7-9-11-17-33(37)40-25-19-21-29-31(23-25)42-32-24-26(41-34(38)18-12-10-8-6-4-2)20-22-30(32)36(29)28-16-14-13-15-27(28)35(39)43-36/h13-16,19-24H,3-12,17-18H2,1-2H3
Fluorescein dioctanoate is a non-fluorescent hydrophobic fluorescein derivative that can pass through the cell membrane whereupon intracellular esterases hydrolyze the dioctanoate group producing the highly fluorescent product fluorescein. The fluorescein molecules accumulate in cells that possess intact membranes so the green fluorescence can be used as a marker of cell viability. Cells that do not possess an intact cell membrane or an active metabolism may not accumulate the fluorescent product and therefore do not exhibit green fluorescence. Fluorescein dioctanoate may be used in combination with PI staining as the non-viable cells take up the PI and stain dead cells red whereas viable cells do not take up the PI and should only stain green. Fluorescein dioctanoate is a fluorogenic substrate for C8 esterase that generates green fluorescence upon esterase cleavage. Spectral Data: &lambda,Ex 497nM, &lamda,Em 516nm. Extinction coefficient: 80000 cm-1 M-1.
Additional information
| Synonyms | FDO, Fluorescein dicaprylate, 3-Oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3',6'-diyl ester octanoic acid |
|---|---|
| Purity | ≥98% (NMR) |
| Appearance | Off-white low melting solid |
| CAS-Number | 19722-86-2 |
| Molecular Formula | C36H40O7 |
| Molecular Weight | 584.72 |
| Identity | 1H-NMR |
| Solvents | DMSO (5 mg/ml) |
| Melting Point | 49 °C |
| Smiles | O=C(O1)C2=C(C=CC=C2)C31C4=CC=C(OC(CCCCCCC)=O)C=C4OC5=C3C=CC(OC(CCCCCCC)=O)=C5 |
| InChi Key | UWZNXQHGTKMTSX-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Transportation | Not dangerous goods |
| References | (1) J.J. Thomas, et al., Exp. Biol. Med. 140, 179 (1972), (2) F.-Y. Ge, et al., Dyes Pigm. 72, 322 (2007), (3) M. Poznika & B. Koeniga, Org. Biomol. Chem. 12, 3175 (2014) |
| InChi | InChI=1S/C36H40O7/c1-3-5-7-9-11-17-33(37)40-25-19-21-29-31(23-25)42-32-24-26(41-34(38)18-12-10-8-6-4-2)20-22-30(32)36(29)28-16-14-13-15-27(28)35(39)43-36/h13-16,19-24H,3-12,17-18H2,1-2H3 |
| Quantity | 100 mg, 250 mg, Bulk |
