Fluorescein (free acid)

Available from stock

Fluorescein (free acid)

SKU
F0023

Categories: ,

Synonyms
Acid Yellow 73 , NSC 667256 , 3',6'-Fluorandiol
2321-07-5
CAS-Number
C20H12O5
Molecular Formula
332.31
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Specifications

Purity
≥99% (HPLC)
Appearance
Red powder
Identity
1H-NMR

Properties

Solvents
acetone or DMSO, water or chloroform
Melting Point
320 °C (lit.)
Boiling Point
620.8 °C at 760 mmHg
Refractive Index
n20D 1.51
Density
0.96 g/cm3
Fluorescence
λex 490 nm, λem 514 nm in DMSO
Shipping
AMBIENT
Short Term Storage
RT
Long Term Storage
RT
Handling Advice
Protect from light and moisture.
Use / Stability
Stable for at least 2 years after receipt when stored at RT.
Hazard statements
H319
Precautionary statements
P305 + P351 + P338
GHS Symbol
GHS07
Signal word
Warning
Transportation
Not dangerous goods
Description
Fluorescein is a fluorophore commonly used in microscopy, flow cytometry forensics, serology to detect latent blood stains, and in dye tracing (attached to certain biologically active molecules). This fluorescent dye/probe has spectral data of ?ex 490 nm| ?em 514 nm in 0.1 M Tris pH 8.0 and is strongly pH dependent. It has an isosbestic point (equal absorption for all pH values) at 460 nm. Fluorescein derivatives are used in different applications in cell biology.
Smiles
O=C(C1=C2C=CC=C1)OC32C4=C(C=C(O)C=C4)OC5=CC(O)=CC=C53
InChi Key
GNBHRKFJIUUOQI-UHFFFAOYSA-N
References
(1) R.F. Chen , Arch. Biochem. Biophys. 133, 263 (1969) , (2) R. Sjöback, et al.: Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 51, L7 (1995) , (3) B.L. Drees, et al.: J. Biol. Chem. 271, 32168 (1996) , (4) M. Taki, et al.: Prot. Eng. Des. Select. 17, 119 (2004)
InChi
InChI=1S/C20H12O5/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20/h1-10,21-22H

Fluorescein is a fluorophore commonly used in microscopy, flow cytometry forensics, serology to detect latent blood stains, and in dye tracing (attached to certain biologically active molecules). This fluorescent dye/probe has spectral data of ?ex 490 nm| ?em 514 nm in 0.1 M Tris pH 8.0 and is strongly pH dependent. It has an isosbestic point (equal absorption for all pH values) at 460 nm. Fluorescein derivatives are used in different applications in cell biology.

SKU: F0023 Categories: ,

Additional information

Synonyms

Acid Yellow 73, NSC 667256, 3',6'-Fluorandiol
Purity

≥99% (HPLC)
Appearance

Red powder
CAS-Number

2321-07-5
Molecular Formula

C20H12O5
Molecular Weight

332.31
Identity

1H-NMR
Solvents

acetone or DMSO, water or chloroform
Melting Point

320 °C (lit.)
Boiling Point

620.8 °C at 760 mmHg
Refractive Index

n20D 1.51
Density

0.96 g/cm3
Fluorescence

λex 490 nm, λem 514 nm in DMSO
Smiles

O=C(C1=C2C=CC=C1)OC32C4=C(C=C(O)C=C4)OC5=CC(O)=CC=C53
InChi Key

GNBHRKFJIUUOQI-UHFFFAOYSA-N
Shipping

AMBIENT
Short Term Storage

RT
Long Term Storage

RT
Handling Advice

Protect from light and moisture.
Use / Stability

Stable for at least 2 years after receipt when stored at RT.
Hazard statements

H319
Precautionary statements

P305 + P351 + P338
GHS Symbol

GHS07
Signal word

Warning
Transportation

Not dangerous goods
References

(1) R.F. Chen, Arch. Biochem. Biophys. 133, 263 (1969), (2) R. Sjöback, et al.: Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 51, L7 (1995), (3) B.L. Drees, et al.: J. Biol. Chem. 271, 32168 (1996), (4) M. Taki, et al.: Prot. Eng. Des. Select. 17, 119 (2004)
InChi

InChI=1S/C20H12O5/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20/h1-10,21-22H
Quantity

250 g, 500 g, 1 kg, Bulk

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