FURA 2-AM

SKU: F0014

Category: Fluorescent Detection

Synonyms: Fura-2 acetoxymethyl ester , Fura-2 pentakis(acetoxymethyl) ester; 108964-32-5
CAS-Number: C₄₄H₄₇N₃O₂₄
Molecular Formula: 1001.87
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥95% (HPLC)
Identity: 1H-NMR
Appearance: Yellow solid

Properties

Optical Activity: Wavelength 370 +/-3 nm
Solvents: DMSO

Downloads

Safety Data Sheet: CDX F0014 MSDS.pdf

Short Term Storage: -20°C
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.
Handling Advice: Protect from light and moisture.
Long Term Storage: -20°C
Shipping: AMBIENT

Transportation: Not dangerous goods

Description: The fluorescent probe FURA 2/AM is a membrane-permeable derivative of the calcium chelator FURA 2. Inside cells, FURA 2/AM is rapidly hydrolyzed by cytoplasmic esterases to FURA 2, which remains within the cell. Non-invasive, intracellular calcium indicator that is UV light-excitable. Fluorescent Ca2+ indicator, selective for Ca2+ over other divalent cations Mg2+, Zn2+, Fe2+ and Mn2+. FURA 2/AM is used to determine calcium concentrations in many cellular systems, including: cardiac myocytes, vascular smooth muscle cells and medullary collecting tubules. Spectral properties in the presence of low calcium concentrations (?ex = 362nm, ?em = 512nm) differ from those during high calcium conditions (?ex = 335nm, ?em = 505nm). FURA 2 is preferred for ratio-imaging microscopy, in which it is more practical to change excitation wavelengths than emission wavelengths. Spectral Data: λex 355nm, λem 495nm in 0.1 M Tris pH 8.0.
InChi: InChI=1S/C44H47N3O24/c1-25-7-8-32(46(16-39(53)65-20-60-26(2)48)17-40(54)66-21-61-27(3)49)35(11-25)58-9-10-59-36-12-31-13-37(43-45-15-38(71-43)44(57)69-24-64-30(6)52)70-34(31)14-33(36)47(18-41(55)67-22-62-28(4)50)19-42(56)68-23-63-29(5)51/h7-8,11-15H,9-10,16-24H2,1-6H3
References: (1) G. Grynkiewicz, et al., J. Biol. Chem. 260, 3440 (1985) , (2) I. Maruyama, et al., FEBS Lett. 220, 89 (1987) , (3) M.E. Janconi, et al., J. Cell Biol. 110, 1555 (1990) , (4) Y. Schwartz, et al., Endocrinology 131, 772 (1992) , (5) G.H. Rao, et al., Biochem. Med. Metab. Biol. 47, 226 (1992) , (6) M.J.Davis, et al., Am. J. Physiol. 263, H1292 (1992) , (7) M.J. Davis, et al., Am. J. Physiol. 263, H1292 (1992) , (8) M.L. Field, et al., Cell Calcium. 16, 87 (1994) , (9) R.W. Sabnis, Handbook of biological dyes and stains (2010)
InChi Key: VPSRLGDRGCKUTK-UHFFFAOYSA-N
Smiles: O=C(OCOC(C)=O)C1=CN=C(O1)C2=CC3=C(O2)C=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OCCOC4=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=CC(C)=C4)=C3

The fluorescent probe FURA 2/AM is a membrane-permeable derivative of the calcium chelator FURA 2. Inside cells, FURA 2/AM is rapidly hydrolyzed by cytoplasmic esterases to FURA 2, which remains within the cell. Non-invasive, intracellular calcium indicator that is UV light-excitable. Fluorescent Ca2+ indicator, selective for Ca2+ over other divalent cations Mg2+, Zn2+, Fe2+ and Mn2+. FURA 2/AM is used to determine calcium concentrations in many cellular systems, including: cardiac myocytes, vascular smooth muscle cells and medullary collecting tubules. Spectral properties in the presence of low calcium concentrations (?ex = 362nm, ?em = 512nm) differ from those during high calcium conditions (?ex = 335nm, ?em = 505nm). FURA 2 is preferred for ratio-imaging microscopy, in which it is more practical to change excitation wavelengths than emission wavelengths. Spectral Data: λex 355nm, λem 495nm in 0.1 M Tris pH 8.0.

SKU: F0014 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	Fura-2 acetoxymethyl ester, Fura-2 pentakis(acetoxymethyl) ester
Purity	≥95% (HPLC)
Appearance	Yellow solid
CAS-Number	108964-32-5
Molecular Formula	C44H47N3O24
Molecular Weight	1001.87
Identity	1H-NMR
Solvents	DMSO
Optical Activity	Wavelength 370 +/-3 nm
Smiles	O=C(OCOC(C)=O)C1=CN=C(O1)C2=CC3=C(O2)C=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OCCOC4=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=CC(C)=C4)=C3
InChi Key	VPSRLGDRGCKUTK-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	-20°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Transportation	Not dangerous goods
References	(1) G. Grynkiewicz, et al., J. Biol. Chem. 260, 3440 (1985), (2) I. Maruyama, et al., FEBS Lett. 220, 89 (1987), (3) M.E. Janconi, et al., J. Cell Biol. 110, 1555 (1990), (4) Y. Schwartz, et al., Endocrinology 131, 772 (1992), (5) G.H. Rao, et al., Biochem. Med. Metab. Biol. 47, 226 (1992), (6) M.J.Davis, et al., Am. J. Physiol. 263, H1292 (1992), (7) M.J. Davis, et al., Am. J. Physiol. 263, H1292 (1992), (8) M.L. Field, et al., Cell Calcium. 16, 87 (1994), (9) R.W. Sabnis, Handbook of biological dyes and stains (2010)
InChi	InChI=1S/C44H47N3O24/c1-25-7-8-32(46(16-39(53)65-20-60-26(2)48)17-40(54)66-21-61-27(3)49)35(11-25)58-9-10-59-36-12-31-13-37(43-45-15-38(71-43)44(57)69-24-64-30(6)52)70-34(31)14-33(36)47(18-41(55)67-22-62-28(4)50)19-42(56)68-23-63-29(5)51/h7-8,11-15H,9-10,16-24H2,1-6H3
Quantity	1 mg, Bulk