Specifications
- Purity
- ≥95% (HPLC)
- Appearance
- Yellow solid
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO
- Optical Activity
- Wavelength 370 +/-3 nm
Documentation
- Safety Data Sheet (SDS)
- CDX F0014 MSDS.pdf
Available from stock
FURA 2-AM
- SKU
- F0014
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- Fura-2 acetoxymethyl ester , Fura-2 pentakis(acetoxymethyl) ester
- 108964-32-5
- CAS-Number
- C44H47N3O24
- Molecular Formula
- 1001.87
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- -20°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Transportation
- Not dangerous goods
- Description
- The fluorescent probe FURA 2/AM is a membrane-permeable derivative of the calcium chelator FURA 2. Inside cells, FURA 2/AM is rapidly hydrolyzed by cytoplasmic esterases to FURA 2, which remains within the cell. Non-invasive, intracellular calcium indicator that is UV light-excitable. Fluorescent Ca2+ indicator, selective for Ca2+ over other divalent cations Mg2+, Zn2+, Fe2+ and Mn2+. FURA 2/AM is used to determine calcium concentrations in many cellular systems, including: cardiac myocytes, vascular smooth muscle cells and medullary collecting tubules. Spectral properties in the presence of low calcium concentrations (?ex = 362nm, ?em = 512nm) differ from those during high calcium conditions (?ex = 335nm, ?em = 505nm). FURA 2 is preferred for ratio-imaging microscopy, in which it is more practical to change excitation wavelengths than emission wavelengths. Spectral Data: λex 355nm, λem 495nm in 0.1 M Tris pH 8.0.
- Smiles
- O=C(OCOC(C)=O)C1=CN=C(O1)C2=CC3=C(O2)C=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OCCOC4=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=CC(C)=C4)=C3
- InChi Key
- VPSRLGDRGCKUTK-UHFFFAOYSA-N
- References
- (1) G. Grynkiewicz, et al., J. Biol. Chem. 260, 3440 (1985) , (2) I. Maruyama, et al., FEBS Lett. 220, 89 (1987) , (3) M.E. Janconi, et al., J. Cell Biol. 110, 1555 (1990) , (4) Y. Schwartz, et al., Endocrinology 131, 772 (1992) , (5) G.H. Rao, et al., Biochem. Med. Metab. Biol. 47, 226 (1992) , (6) M.J.Davis, et al., Am. J. Physiol. 263, H1292 (1992) , (7) M.J. Davis, et al., Am. J. Physiol. 263, H1292 (1992) , (8) M.L. Field, et al., Cell Calcium. 16, 87 (1994) , (9) R.W. Sabnis, Handbook of biological dyes and stains (2010)
- InChi
- InChI=1S/C44H47N3O24/c1-25-7-8-32(46(16-39(53)65-20-60-26(2)48)17-40(54)66-21-61-27(3)49)35(11-25)58-9-10-59-36-12-31-13-37(43-45-15-38(71-43)44(57)69-24-64-30(6)52)70-34(31)14-33(36)47(18-41(55)67-22-62-28(4)50)19-42(56)68-23-63-29(5)51/h7-8,11-15H,9-10,16-24H2,1-6H3
The fluorescent probe FURA 2/AM is a membrane-permeable derivative of the calcium chelator FURA 2. Inside cells, FURA 2/AM is rapidly hydrolyzed by cytoplasmic esterases to FURA 2, which remains within the cell. Non-invasive, intracellular calcium indicator that is UV light-excitable. Fluorescent Ca2+ indicator, selective for Ca2+ over other divalent cations Mg2+, Zn2+, Fe2+ and Mn2+. FURA 2/AM is used to determine calcium concentrations in many cellular systems, including: cardiac myocytes, vascular smooth muscle cells and medullary collecting tubules. Spectral properties in the presence of low calcium concentrations (?ex = 362nm, ?em = 512nm) differ from those during high calcium conditions (?ex = 335nm, ?em = 505nm). FURA 2 is preferred for ratio-imaging microscopy, in which it is more practical to change excitation wavelengths than emission wavelengths. Spectral Data: λex 355nm, λem 495nm in 0.1 M Tris pH 8.0.
Additional information
| Synonyms | Fura-2 acetoxymethyl ester, Fura-2 pentakis(acetoxymethyl) ester |
|---|---|
| Purity | ≥95% (HPLC) |
| Appearance | Yellow solid |
| CAS-Number | 108964-32-5 |
| Molecular Formula | C44H47N3O24 |
| Molecular Weight | 1001.87 |
| Identity | 1H-NMR |
| Solvents | DMSO |
| Optical Activity | Wavelength 370 +/-3 nm |
| Smiles | O=C(OCOC(C)=O)C1=CN=C(O1)C2=CC3=C(O2)C=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OCCOC4=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=CC(C)=C4)=C3 |
| InChi Key | VPSRLGDRGCKUTK-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | -20°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Transportation | Not dangerous goods |
| References | (1) G. Grynkiewicz, et al., J. Biol. Chem. 260, 3440 (1985), (2) I. Maruyama, et al., FEBS Lett. 220, 89 (1987), (3) M.E. Janconi, et al., J. Cell Biol. 110, 1555 (1990), (4) Y. Schwartz, et al., Endocrinology 131, 772 (1992), (5) G.H. Rao, et al., Biochem. Med. Metab. Biol. 47, 226 (1992), (6) M.J.Davis, et al., Am. J. Physiol. 263, H1292 (1992), (7) M.J. Davis, et al., Am. J. Physiol. 263, H1292 (1992), (8) M.L. Field, et al., Cell Calcium. 16, 87 (1994), (9) R.W. Sabnis, Handbook of biological dyes and stains (2010) |
| InChi | InChI=1S/C44H47N3O24/c1-25-7-8-32(46(16-39(53)65-20-60-26(2)48)17-40(54)66-21-61-27(3)49)35(11-25)58-9-10-59-36-12-31-13-37(43-45-15-38(71-43)44(57)69-24-64-30(6)52)70-34(31)14-33(36)47(18-41(55)67-22-62-28(4)50)19-42(56)68-23-63-29(5)51/h7-8,11-15H,9-10,16-24H2,1-6H3 |
| Quantity | 1 mg, Bulk |
