Specifications
- Purity
- for microscopy
- Appearance
- Purple to very dark purple liquid
- Identity
- UV/Vis
- UV/Vis
- 585 - 595 nm
- Density
- 0.979 - 0.983
Properties
- Density
- 0.981 g/ml
- Solvents
- water
Downloads
- Safety Data Sheet
- CDX G0060 MSDS.pdf
Available from stock
Gram’s Crystal Violet Solution
- SKU
- G0060
Category: Non Fluorescent Detection & Chromogene Detection
- 548-62-9 (comp.)
- CAS-Number
- n/a
- Molecular Formula
- n/a
- Molecular Weight
- Use / Stability
- This product should not be used if colour has changed or if there are
- Handling Advice
- Protect from light and moisture.
- Long Term Storage
- RT
- Short Term Storage
- RT
- Shipping
- AMBIENT
- Hazard statements
- H226-H319-H351-H411
- Transportation
- Packing Group II
- RIDADR
- UN1170
- Signal word
- Warning
- GHS Symbol
- GHS02+GHS07+08+09
- Precautionary statements
- P273-P281-P305 + P351 + P338
- Description
- Gram Crystal Violet Solution is a reagent recommended for use in qualitative procedures to differentiate gram-negative from gram-positive organisms. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution (Lugol’s solution), a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by safranin solution and appear orange.
- References
- (1) E. Adams, Stain Technol. 50, 227 (1975) , (2) R. Coico, et al., Curr. Protoc. Microbiol. Appendix 3-3C (2005)
- Smiles
- CN(C)C1=CC=C(/C(C2=CC=C(N(C)C)C=C2)=C3C=C/C(C=C/3)=[N+](C)/C)C=C1.[Cl-]
Gram Crystal Violet Solution is a reagent recommended for use in qualitative procedures to differentiate gram-negative from gram-positive organisms. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram’s crystal violet solution – an aniline dye – in the first step. After the treatment with iod solution (Lugol’s solution), a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by safranin solution and appear orange.
Additional information
| Purity | for microscopy |
|---|---|
| Appearance | Purple to very dark purple liquid |
| CAS-Number | 548-62-9 (comp.) |
| Molecular Formula | n/a |
| Molecular Weight | n/a |
| Identity | UV/Vis |
| Solvents | water |
| Density | 0.981 g/ml |
| Smiles | CN(C)C1=CC=C(/C(C2=CC=C(N(C)C)C=C2)=C3C=C/C(C=C/3)=[N+](C)/C)C=C1.[Cl-] |
| Shipping | AMBIENT |
| Short Term Storage | RT |
| Long Term Storage | RT |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | This product should not be used if colour has changed or if there are |
| Hazard statements | H226-H319-H351-H411 |
| Precautionary statements | P273-P281-P305 + P351 + P338 |
| GHS Symbol | GHS02+GHS07+08+09 |
| Signal word | Warning |
| RIDADR | UN1170 |
| Transportation | Packing Group II |
| References | (1) E. Adams, Stain Technol. 50, 227 (1975), (2) R. Coico, et al., Curr. Protoc. Microbiol. Appendix 3-3C (2005) |
| Quantity | 250 ml, 2.5 l, Bulk |
| cdx-UV/Vis | 585 – 595 nm |
| cdx-Density | 0.979 – 0.983 |
