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    Gram’s Crystal Violet Solution

    Available from stock

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      Gram’s Crystal Violet Solution

      SKU
      G0060

      Category: Non Fluorescent Detection & Chromogene Detection

      548-62-9 (comp.)
      CAS-Number
      n/a
      Molecular Formula
      n/a
      Molecular Weight

      For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

      • Product Data
      • Handling
      • Safety Information
      • Details

      Specifications

      Purity
      for microscopy
      Appearance
      Purple to very dark purple liquid
      Identity
      UV/Vis
      UV/Vis
      585 - 595 nm
      Density
      0.979 - 0.983

      Properties

      Solvents
      water
      Density
      0.981 g/ml

      Documentation

      Safety Data Sheet (SDS)
      CDX G0060 MSDS.pdf
      Shipping
      AMBIENT
      Short Term Storage
      RT
      Long Term Storage
      RT
      Handling Advice
      Protect from light and moisture.
      Use / Stability
      This product should not be used if colour has changed or if there are
      Hazard statements
      H226-H319-H351-H411
      Precautionary statements
      P273-P281-P305 + P351 + P338
      GHS Symbol
      GHS02+GHS07+08+09
      Signal word
      Warning
      RIDADR
      UN1170
      Transportation
      Packing Group II
      Description
      Gram Crystal Violet Solution is a reagent recommended for use in qualitative procedures to differentiate gram-negative from gram-positive organisms. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution (Lugol’s solution), a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by safranin solution and appear orange.
      Smiles
      CN(C)C1=CC=C(/C(C2=CC=C(N(C)C)C=C2)=C3C=C/C(C=C/3)=[N+](C)/C)C=C1.[Cl-]
      References
      (1) E. Adams, Stain Technol. 50, 227 (1975) , (2) R. Coico, et al., Curr. Protoc. Microbiol. Appendix 3-3C (2005)

      Gram Crystal Violet Solution is a reagent recommended for use in qualitative procedures to differentiate gram-negative from gram-positive organisms. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram’s crystal violet solution – an aniline dye – in the first step. After the treatment with iod solution (Lugol’s solution), a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by safranin solution and appear orange.

      SKU: G0060 Category: Non Fluorescent Detection & Chromogene Detection
      • Additional information

      Additional information

      Purity

      for microscopy

      Appearance

      Purple to very dark purple liquid

      CAS-Number

      548-62-9 (comp.)

      Molecular Formula

      n/a

      Molecular Weight

      n/a

      Identity

      UV/Vis

      Solvents

      water

      Density

      0.981 g/ml

      Smiles

      CN(C)C1=CC=C(/C(C2=CC=C(N(C)C)C=C2)=C3C=C/C(C=C/3)=[N+](C)/C)C=C1.[Cl-]

      Shipping

      AMBIENT

      Short Term Storage

      RT

      Long Term Storage

      RT

      Handling Advice

      Protect from light and moisture.

      Use / Stability

      This product should not be used if colour has changed or if there are

      Hazard statements

      H226-H319-H351-H411

      Precautionary statements

      P273-P281-P305 + P351 + P338

      GHS Symbol

      GHS02+GHS07+08+09

      Signal word

      Warning

      RIDADR

      UN1170

      Transportation

      Packing Group II

      References

      (1) E. Adams, Stain Technol. 50, 227 (1975), (2) R. Coico, et al., Curr. Protoc. Microbiol. Appendix 3-3C (2005)

      Quantity

      250 ml, 2.5 l, Bulk

      cdx-UV/Vis

      585 – 595 nm

      cdx-Density

      0.979 – 0.983

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