Available from stock

Gram’s Safranin Solution

SKU: G0061

Category: Non Fluorescent Detection & Chromogene Detection

Synonyms: Safranin O Solution , Safarin T Solution; 477-73-6 (comp.)
CAS-Number: C₂₀H₁₉ClN₄
Molecular Formula: 350.84
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: for microscopy
Appearance: Red to very dark red liquid
Identity: UV/Vis
UV/Vis: 525 - 535 nm

Properties

Solvents: ethanol
Refractive Index: n20/D 1.338 - 1.340
Density: 0.986 g/ml

Documentation

Safety Data Sheet (SDS): CDX G0061 MSDS.pdf

Shipping: AMBIENT
Short Term Storage: RT
Long Term Storage: RT
Handling Advice: Protect from light and moisture.
Use / Stability: This product should not be used if colour has changed or if there are

Hazard statements: H226
GHS Symbol: GHS02
Signal word: Warning
Transportation: Packing Group III

Description: Gram Safranin Solution is a reagent recommended for use in qualitative procedures to differentiate gram-negative from gram-positive organisms. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution, a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized. Gram-negative bacteria will be counterstained by safranin solution and appear orange.
Smiles: CC1=C(N)C=C([N+](C2=CC=CC=C2)=C(C=C(N)C(C)=C3)C3=N4)C4=C1.[Cl-]
InChi Key: OARRHUQTFTUEOS-UHFFFAOYSA-N
References: (1) E. Adams, Stain Technol. 50, 227 (1975) , (2) R. Coico, et al., Curr. Protoc. Microbiol. Appendix 3-3C (2005)

Gram Safranin Solution is a reagent recommended for use in qualitative procedures to differentiate gram-negative from gram-positive organisms. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram’s crystal violet solution – an aniline dye – in the first step. After the treatment with iod solution, a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized. Gram-negative bacteria will be counterstained by safranin solution and appear orange.

SKU: G0061 Category: Non Fluorescent Detection & Chromogene Detection

Additional information

Additional information

Synonyms	Safranin O Solution, Safarin T Solution
Purity	for microscopy
Appearance	Red to very dark red liquid
CAS-Number	477-73-6 (comp.)
Molecular Formula	C20H19ClN4
Molecular Weight	350.84
Identity	UV/Vis
Solvents	ethanol
Refractive Index	n20/D 1.338 – 1.340
Density	0.986 g/ml
Smiles	CC1=C(N)C=C([N+](C2=CC=CC=C2)=C(C=C(N)C(C)=C3)C3=N4)C4=C1.[Cl-]
InChi Key	OARRHUQTFTUEOS-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	RT
Long Term Storage	RT
Handling Advice	Protect from light and moisture.
Use / Stability	This product should not be used if colour has changed or if there are
Hazard statements	H226
GHS Symbol	GHS02
Signal word	Warning
Transportation	Packing Group III
References	(1) E. Adams, Stain Technol. 50, 227 (1975), (2) R. Coico, et al., Curr. Protoc. Microbiol. Appendix 3-3C (2005)
Quantity	250 ml, 2.5 l, Bulk
cdx-UV/Vis	525 – 535 nm