Specifications
- Purity
- ≥99% (Titration)
- Appearance
- White to off-white powder
- Identity
- 1H-NMR
Properties
- Solvents
- water (10M), DMSO
- Melting Point
- 118° C - 121° C
Available from stock
Guanidine thiocyanate
- SKU
- G0058
Category: Antibiotics
- Synonyms
- Guanidinium rhodanide , GTC , Guanidinium isothiocyanate , Guanidinium thiocyanate
- 593-84-0
- CAS-Number
- CH5N3 · CHNS
- Molecular Formula
- 59.07 . 59.09
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- RT
- Long Term Storage
- RT
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at RT.
- Hazard statements
- H302 + H312 + H332-H314-H412
- Precautionary statements
- P260-P280-P301 + P312 + P330-P303 + P361 + P353-P304 + P340 + P310-P305 + P351 + P338
- GHS Symbol
- GHS05+GHS07
- Signal word
- Danger
- RIDADR
- UN 1759 8
- Transportation
- Packing Group III
- Description
- Guanidine thiocyanate is a powerful, chaotropic agent which is widely used for purification of proteins and nucleic acids. Guanidine thiocyanate is preferred for the purification of RNA because it dissociates the RNA into its nucleic acids and protein forms and is commonly used in the guanidinium thiocyanate-phenol-chloroform extraction method. As one of the strongest protein denaturants, it inactivates nucleases more than twice as fast as Guanidine hydrochloride. It is useful for denaturation and refolding of proteins. At high molarity solutions, Guanidine thiocyanate irreversibly inactivates RNase. Guanidine thiocyanate causes proteins to dissolve readily, thus disintegrating cellular structures.
- Smiles
- NC(N)=N.SC#N
- InChi Key
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N
- References
- (1) S. Lapanje, Biochim. Biophys. Acta 243, 349 (1971) , (2) S. Lapanje, Biochim. Biophys. Acta 243, 357 (1971) , (3) W.C. Johnson, et al., Appl. Microbiol. 29, 405 (1975) , (4) R. McGookin, Methods Mol. Biol. 2, 113 (1985) , (5) P. Chomczynski, et al., Anal. Biochem. 162, 156 (1987) , (6) T. Nakano & A.L. Fink, J. Biol. Chem. 265, 12356 (1990) , (7) S. Emadi & M. Behzadi, BBRC 450, 1339 (2014)
- InChi
- InChI=1S/CH5N3.CHNS/c2-1(3)4 , 2-1-3/h(H5,2,3,4) , 3H
Guanidine thiocyanate is a powerful, chaotropic agent which is widely used for purification of proteins and nucleic acids. Guanidine thiocyanate is preferred for the purification of RNA because it dissociates the RNA into its nucleic acids and protein forms and is commonly used in the guanidinium thiocyanate-phenol-chloroform extraction method. As one of the strongest protein denaturants, it inactivates nucleases more than twice as fast as Guanidine hydrochloride. It is useful for denaturation and refolding of proteins. At high molarity solutions, Guanidine thiocyanate irreversibly inactivates RNase. Guanidine thiocyanate causes proteins to dissolve readily, thus disintegrating cellular structures.
Additional information
| Synonyms | Guanidinium rhodanide, GTC, Guanidinium isothiocyanate, Guanidinium thiocyanate |
|---|---|
| Purity | ≥99% (Titration) |
| Appearance | White to off-white powder |
| CAS-Number | 593-84-0 |
| Molecular Formula | CH5N3 · CHNS |
| Molecular Weight | 59.07 . 59.09 |
| Identity | 1H-NMR |
| Solvents | water (10M), DMSO |
| Melting Point | 118° C – 121° C |
| Smiles | NC(N)=N.SC#N |
| InChi Key | ZJYYHGLJYGJLLN-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | RT |
| Long Term Storage | RT |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at RT. |
| Hazard statements | H302 + H312 + H332-H314-H412 |
| Precautionary statements | P260-P280-P301 + P312 + P330-P303 + P361 + P353-P304 + P340 + P310-P305 + P351 + P338 |
| GHS Symbol | GHS05+GHS07 |
| Signal word | Danger |
| RIDADR | UN 1759 8 |
| Transportation | Packing Group III |
| References | (1) S. Lapanje, Biochim. Biophys. Acta 243, 349 (1971), (2) S. Lapanje, Biochim. Biophys. Acta 243, 357 (1971), (3) W.C. Johnson, et al., Appl. Microbiol. 29, 405 (1975), (4) R. McGookin, Methods Mol. Biol. 2, 113 (1985), (5) P. Chomczynski, et al., Anal. Biochem. 162, 156 (1987), (6) T. Nakano & A.L. Fink, J. Biol. Chem. 265, 12356 (1990), (7) S. Emadi & M. Behzadi, BBRC 450, 1339 (2014) |
| InChi | InChI=1S/CH5N3.CHNS/c2-1(3)4, 2-1-3/h(H5,2,3,4), 3H |
| Quantity | 100 g, 500 g, Bulk |
