Specifications
- Purity
- ≥95% (HPLC)
- Appearance
- Yellow solid
Properties
- Solvents
- water, DMSO (20mg/ml).
Category: Fluorescent Detection, Fluorescent Detection
Hydroxystilbamidine (also called Fluoro Gold) is a cationic dye used for staining DNA and RNA. It exhibits distinctively different fluorescence emission profiles when bound to DNA compared to RNA. It is also frequently used as a retrograde neuronal tracer. The fluorescent tracer provides intense retrograde labeling that is extremely sensitive and reliable, does not diffuse out of retrograde-labeled neurons, and can be pressure-injected or introduced by iontophoresis into cells. Hydroxystilbamidine is compatible with most frequently used neuroanatomical techniques such as immunofluorescence, immunocytochemistry, autoradiography, and horseradish peroxidase-based histochemistry, paraffin embedding. Gold color is emitted when tissue is processed with a neutral pH buffer while blue/bright white is emitted when an acidic pH buffer is used. Spectral data: λEx=361nm and λEm=536nm. In addition, hydroxystilbamidine is an amidine antibiotic with antibacterial antifungal, antitrypanosomal, antimalarial and carcinostatic activities. Binds to DNA and RNA in a non-intercalating manner, and is a powerful inhibitor of ribonucleases. Binds also to lysosomes.
Synonyms | 2-Hydroxystilbene-4,4'-dicarboxamidine bis(methanesulfonate), Fluoro Gold |
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Purity | ≥95% (HPLC) |
Appearance | Yellow solid |
CAS-Number | 223769-64-0 |
Molecular Formula | C16H16N4O . 2CH4O3S |
Molecular Weight | 280.3 . 192.2 |
Solvents | water, DMSO (20mg/ml). |
Smiles | OC1=C(/C=C/C2=CC=C(C([NH3+])=N)C=C2)C=CC(C([NH3+])=N)=C1.CS(=O)([O-])=O.CS(=O)([O-])=O |
InChi Key | YGNSQKCULHSJDC-HFPMQDOPSA-N |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | +4°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
Hazard statements | H315-H319-H335 |
Precautionary statements | P261-P305 + P351 + P338 |
GHS Symbol | GHS07 |
Transportation | Not dangerous goods |
References | (1) C.L. Taschdjian, J. Invest. Dermatol. 22, 521 (1954), (2) E. Delain, et al., J. Ultrastruct. Res. 37, 200 (1971), (3) B. Festy & M. Daune, Biochemistry 12, 4827 (1973), (4) P.M. Lizardi, J. Cell Biol. 87, 292 (1980), (5) L.B. Murgatroyd, Histochemistry 74, 107 (1982), (6) L.B. Murgatroyd, Diagn. Histopathol. 5, 219 (1982), (7) L.C. Schmued & J.H. Fallon, Brain Res. 377, 147 (1986), (8) L.C. Schmued, et al., J. Neurocytol. 18, 333 (1989), (9) L.C. Schmued & L. Heimer, J. Histochem. Cytochem. 38, 721 (1990), (10) M.W. Wessendorf, Brain Res 553, 135 (1991), (11) J.F. Bowyer, et al., J. Pharmacol. Exp. Ther. 266, 1066 (1993), (12) A. Puigdellivol-Sanchez, et al., J. Neurosci. Method. 86, 7 (1998), (13) L.A. Catapano, et al., Methods Mol. Biol. 438, 353 (2008) |
Quantity | 10 mg, Bulk |