• Home
Search

Results for {phrase} ({results_count} of {results_count_total})

Displaying {results_count} results of {results_count_total}

More results...

Generic filters
Exact matches only
    Lindenstrasse 77, CH-9000 St. Gallen, Tel: +41 71 244 48 25
    • 0Shopping Cart
    Chemodex
    • Home
    • Products
    • Catalogs
    • About Chemodex
    • Contact & Distributors
    • Menu
    Indo 1-AM

    Available from stock

    Inquiry

    Please fill out the form for your inquiry:



    * required field

    Indo 1-AM

    SKU
    I0019

    Category: Fluorescent Detection

    Synonyms
    Indo-1-(acetoxymethyl) ester , 4-(6-Carboxy-2-indolyl)-4'-methyl-2,2'-(ethylenedioxy)dianiline-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl) ester
    112926-02-0
    CAS-Number
    C47H51N3O22
    Molecular Formula
    1009.93
    Molecular Weight

    • Product Data
    • Handling
    • Safety Information
    • Details

    Specifications

    Purity
    ≥90% (HPLC)
    Appearance
    Off-white solid
    Purity 2
    ≥90% (TLC)

    Properties

    Solvents
    DMSO, methanol
    Long Term Storage
    -20°C
    Use / Stability
    Stable for at least 2 years after receipt when stored at -20°C.
    Handling Advice
    Protect from light and moisture.
    Short Term Storage
    -20°C
    Shipping
    AMBIENT
    Transportation
    Not dangerous goods
    Description
    INDO 1/AM is a popular non-invasive UV-excitable calcium indicator. It is the cell-permeable ester derivative of INDO 1. After crossing the cell membrane, INDO 1/AM is rapidly hydrolyzed by cytoplasmic esterases to produce the ratiometric fluorescent calcium indicator INDO 1, which remains trapped within the cell. INDO 1/AM has been used to selectively monitor Ca2+ levels in mitochondria and in the cytosol. INDO 1 is ideal for analyses using flow cytometry, as it uses a single excitation source (usually the 351-364nm spectral lines of the argon-ion laser). In contrast to FURA 1, INDO 1 has a dual emission peak. The emission of INDO 1 shifts to 400nm (when bound to Ca2+) from 480nm in Ca2+-free environments. INDO 1 is prone to photobleaching, which limits its usefulness in methods involving microscopy. Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Spectral Data: λex 330nm, λem 450nm (with Calcium), λex 356nm, λem 478nm in methanol.
    InChi
    InChI=1S/C47H51N3O22/c1-28-7-11-39(49(19-43(56)68-23-63-29(2)51)20-44(57)69-24-64-30(3)52)41(15-28)61-13-14-62-42-18-35(37-16-34-8-9-36(17-38(34)48-37)47(60)72-27-67-33(6)55)10-12-40(42)50(21-45(58)70-25-65-31(4)53)22-46(59)71-26-66-32(5)54/h7-12,15-18,48H,13-14,19-27H2,1-6H3
    References
    (1) G. Grynkiewicz, et al., J. Biol. Chem. 260, 3440 (1985) , (2) M. Lopez, et al., Cytometry 10, 165 (1989) , (3) J.P. Grierson, et al., J. Neurophysiol. 67, 704 (1992) , (4) E.J. Griffiths, et al., Am. J. Physiol. 273, C37 (1997) , (5) R.M. Paredes, et al., Meth. A Comp. Meth. Enzymol. 46, 143 (2008) , (6) Z. Zhou, et al., J. Physiol. 507, 379 (1998) , (7) S. Bailey & P.J. Macardle, J. Immunol. Meth. 311, 220 (2006) , (8) A. Nelemans, Methods Mol. Biol. 312, 47 (2006) , (9) R.W. Sabnis, Handbook of biological dyes and stains (2010)
    InChi Key
    CAWBRCOBJNWRLK-UHFFFAOYSA-N
    Smiles
    CC1=CC(OCCOC2=CC(C3=CC(C=CC(C(OCOC(C)=O)=O)=C4)=C4N3)=CC=C2N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=C1

    INDO 1/AM is a popular non-invasive UV-excitable calcium indicator. It is the cell-permeable ester derivative of INDO 1. After crossing the cell membrane, INDO 1/AM is rapidly hydrolyzed by cytoplasmic esterases to produce the ratiometric fluorescent calcium indicator INDO 1, which remains trapped within the cell. INDO 1/AM has been used to selectively monitor Ca2+ levels in mitochondria and in the cytosol. INDO 1 is ideal for analyses using flow cytometry, as it uses a single excitation source (usually the 351-364nm spectral lines of the argon-ion laser). In contrast to FURA 1, INDO 1 has a dual emission peak. The emission of INDO 1 shifts to 400nm (when bound to Ca2+) from 480nm in Ca2+-free environments. INDO 1 is prone to photobleaching, which limits its usefulness in methods involving microscopy. Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Spectral Data: λex 330nm, λem 450nm (with Calcium), λex 356nm, λem 478nm in methanol.

    SKU: I0019 Category: Fluorescent Detection
    • Additional information

    Additional information

    Synonyms

    Indo-1-(acetoxymethyl) ester, 4-(6-Carboxy-2-indolyl)-4'-methyl-2,2'-(ethylenedioxy)dianiline-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl) ester

    Purity

    ≥90% (HPLC)

    Appearance

    Off-white solid

    CAS-Number

    112926-02-0

    Molecular Formula

    C47H51N3O22

    Molecular Weight

    1009.93

    Solvents

    DMSO, methanol

    Smiles

    CC1=CC(OCCOC2=CC(C3=CC(C=CC(C(OCOC(C)=O)=O)=C4)=C4N3)=CC=C2N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=C1

    InChi Key

    CAWBRCOBJNWRLK-UHFFFAOYSA-N

    Shipping

    AMBIENT

    Short Term Storage

    -20°C

    Long Term Storage

    -20°C

    Handling Advice

    Protect from light and moisture.

    Use / Stability

    Stable for at least 2 years after receipt when stored at -20°C.

    Transportation

    Not dangerous goods

    References

    (1) G. Grynkiewicz, et al., J. Biol. Chem. 260, 3440 (1985), (2) M. Lopez, et al., Cytometry 10, 165 (1989), (3) J.P. Grierson, et al., J. Neurophysiol. 67, 704 (1992), (4) E.J. Griffiths, et al., Am. J. Physiol. 273, C37 (1997), (5) R.M. Paredes, et al., Meth. A Comp. Meth. Enzymol. 46, 143 (2008), (6) Z. Zhou, et al., J. Physiol. 507, 379 (1998), (7) S. Bailey & P.J. Macardle, J. Immunol. Meth. 311, 220 (2006), (8) A. Nelemans, Methods Mol. Biol. 312, 47 (2006), (9) R.W. Sabnis, Handbook of biological dyes and stains (2010)

    InChi

    InChI=1S/C47H51N3O22/c1-28-7-11-39(49(19-43(56)68-23-63-29(2)51)20-44(57)69-24-64-30(3)52)41(15-28)61-13-14-62-42-18-35(37-16-34-8-9-36(17-38(34)48-37)47(60)72-27-67-33(6)55)10-12-40(42)50(21-45(58)70-25-65-31(4)53)22-46(59)71-26-66-32(5)54/h7-12,15-18,48H,13-14,19-27H2,1-6H3

    Quantity

    1 mg, Bulk

    cdx-Purity 2

    ≥90% (TLC)

    Related products

    • 10-Acetyl-3

      10-Acetyl-3,7-dihydroxyphenoxazine

      Purity: ≥98% (HPLC)CAS-Number: 119171-73-2SKU: A0022

      Read more Show Details
    • 2-Anthracenylsulfonyl chloride

      2-Anthracenylsulfonyl chloride

      Purity: ≥90% (HPLC)CAS-Number: 17407-98-6SKU: A0062

      Read more Show Details
    • 5(6)-Aminofluorescein (Mixture of isomers)

      5(6)-Aminofluorescein (Mixture of isomers)

      Purity: ≥95% (HPLC)CAS-Number: 27599-63-9SKU: A0039

      Read more Show Details
    • Anthracene-9

      Anthracene-9,10-dipropionic acid disodium salt

      Purity: ≥95% (HPLC)CAS-Number: 82767-90-6SKU: A0116

      Read more Show Details
    © Copyright - Chemodex Ltd. - Customized Molecules developed in Switzerland
    • Home
    • All products
    • Order and delivery information
    • Disclaimer
    • Privacy policy
    • About this site
    HITCI HITCI 6-IAF 6-IAF Scroll to top

    This website makes use of cookies; these are used to enhance user experience and for purposes of web analysis that helps us to improve our website. By continuing to use the website, you consent to our use of cookies.

    AcceptFurther information