Specifications
- Purity
- ≥90% (HPLC)
- Appearance
- Off-white solid
- Purity 2
- ≥90% (TLC)
Properties
- Solvents
- DMSO, methanol
Available from stock
Indo 1-AM
- SKU
- I0019
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- Indo-1-(acetoxymethyl) ester , 4-(6-Carboxy-2-indolyl)-4'-methyl-2,2'-(ethylenedioxy)dianiline-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl) ester
- 112926-02-0
- CAS-Number
- C47H51N3O22
- Molecular Formula
- 1009.93
- Molecular Weight
- Long Term Storage
- -20°C
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Handling Advice
- Protect from light and moisture.
- Short Term Storage
- -20°C
- Shipping
- AMBIENT
- Transportation
- Not dangerous goods
- Description
- INDO 1/AM is a popular non-invasive UV-excitable calcium indicator. It is the cell-permeable ester derivative of INDO 1. After crossing the cell membrane, INDO 1/AM is rapidly hydrolyzed by cytoplasmic esterases to produce the ratiometric fluorescent calcium indicator INDO 1, which remains trapped within the cell. INDO 1/AM has been used to selectively monitor Ca2+ levels in mitochondria and in the cytosol. INDO 1 is ideal for analyses using flow cytometry, as it uses a single excitation source (usually the 351-364nm spectral lines of the argon-ion laser). In contrast to FURA 1, INDO 1 has a dual emission peak. The emission of INDO 1 shifts to 400nm (when bound to Ca2+) from 480nm in Ca2+-free environments. INDO 1 is prone to photobleaching, which limits its usefulness in methods involving microscopy. Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Spectral Data: λex 330nm, λem 450nm (with Calcium), λex 356nm, λem 478nm in methanol.
- InChi
- InChI=1S/C47H51N3O22/c1-28-7-11-39(49(19-43(56)68-23-63-29(2)51)20-44(57)69-24-64-30(3)52)41(15-28)61-13-14-62-42-18-35(37-16-34-8-9-36(17-38(34)48-37)47(60)72-27-67-33(6)55)10-12-40(42)50(21-45(58)70-25-65-31(4)53)22-46(59)71-26-66-32(5)54/h7-12,15-18,48H,13-14,19-27H2,1-6H3
- References
- (1) G. Grynkiewicz, et al., J. Biol. Chem. 260, 3440 (1985) , (2) M. Lopez, et al., Cytometry 10, 165 (1989) , (3) J.P. Grierson, et al., J. Neurophysiol. 67, 704 (1992) , (4) E.J. Griffiths, et al., Am. J. Physiol. 273, C37 (1997) , (5) R.M. Paredes, et al., Meth. A Comp. Meth. Enzymol. 46, 143 (2008) , (6) Z. Zhou, et al., J. Physiol. 507, 379 (1998) , (7) S. Bailey & P.J. Macardle, J. Immunol. Meth. 311, 220 (2006) , (8) A. Nelemans, Methods Mol. Biol. 312, 47 (2006) , (9) R.W. Sabnis, Handbook of biological dyes and stains (2010)
- InChi Key
- CAWBRCOBJNWRLK-UHFFFAOYSA-N
- Smiles
- CC1=CC(OCCOC2=CC(C3=CC(C=CC(C(OCOC(C)=O)=O)=C4)=C4N3)=CC=C2N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=C1
INDO 1/AM is a popular non-invasive UV-excitable calcium indicator. It is the cell-permeable ester derivative of INDO 1. After crossing the cell membrane, INDO 1/AM is rapidly hydrolyzed by cytoplasmic esterases to produce the ratiometric fluorescent calcium indicator INDO 1, which remains trapped within the cell. INDO 1/AM has been used to selectively monitor Ca2+ levels in mitochondria and in the cytosol. INDO 1 is ideal for analyses using flow cytometry, as it uses a single excitation source (usually the 351-364nm spectral lines of the argon-ion laser). In contrast to FURA 1, INDO 1 has a dual emission peak. The emission of INDO 1 shifts to 400nm (when bound to Ca2+) from 480nm in Ca2+-free environments. INDO 1 is prone to photobleaching, which limits its usefulness in methods involving microscopy. Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Spectral Data: λex 330nm, λem 450nm (with Calcium), λex 356nm, λem 478nm in methanol.
Additional information
| Synonyms | Indo-1-(acetoxymethyl) ester, 4-(6-Carboxy-2-indolyl)-4'-methyl-2,2'-(ethylenedioxy)dianiline-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl) ester |
|---|---|
| Purity | ≥90% (HPLC) |
| Appearance | Off-white solid |
| CAS-Number | 112926-02-0 |
| Molecular Formula | C47H51N3O22 |
| Molecular Weight | 1009.93 |
| Solvents | DMSO, methanol |
| Smiles | CC1=CC(OCCOC2=CC(C3=CC(C=CC(C(OCOC(C)=O)=O)=C4)=C4N3)=CC=C2N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=C1 |
| InChi Key | CAWBRCOBJNWRLK-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | -20°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Transportation | Not dangerous goods |
| References | (1) G. Grynkiewicz, et al., J. Biol. Chem. 260, 3440 (1985), (2) M. Lopez, et al., Cytometry 10, 165 (1989), (3) J.P. Grierson, et al., J. Neurophysiol. 67, 704 (1992), (4) E.J. Griffiths, et al., Am. J. Physiol. 273, C37 (1997), (5) R.M. Paredes, et al., Meth. A Comp. Meth. Enzymol. 46, 143 (2008), (6) Z. Zhou, et al., J. Physiol. 507, 379 (1998), (7) S. Bailey & P.J. Macardle, J. Immunol. Meth. 311, 220 (2006), (8) A. Nelemans, Methods Mol. Biol. 312, 47 (2006), (9) R.W. Sabnis, Handbook of biological dyes and stains (2010) |
| InChi | InChI=1S/C47H51N3O22/c1-28-7-11-39(49(19-43(56)68-23-63-29(2)51)20-44(57)69-24-64-30(3)52)41(15-28)61-13-14-62-42-18-35(37-16-34-8-9-36(17-38(34)48-37)47(60)72-27-67-33(6)55)10-12-40(42)50(21-45(58)70-25-65-31(4)53)22-46(59)71-26-66-32(5)54/h7-12,15-18,48H,13-14,19-27H2,1-6H3 |
| Quantity | 1 mg, Bulk |
| cdx-Purity 2 | ≥90% (TLC) |
