Available from stock

Iodonitrotetrazolium chloride

SKU: I0007

Category: Non Fluorescent Detection & Chromogene Detection

Synonyms: p-Iodonitrotetrazolium Violet, INT, 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride, NSC 27620; 146-68-9
CAS-Number: C₁₉H₁₃CIN₅O₂
Molecular Formula: 505.7
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥97% (TLC)
Appearance: White, dark yellow to dark orange powder
Identity: 1H-NMR

Properties

Solvents: methanol (10mg/ml), ethanol (30mg/ml) or water (10mg/ml)
Melting Point: 238-250 °C (dec.)

Shipping: AMBIENT
Short Term Storage: RT
Long Term Storage: +4°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at +4°C.

Hazard statements: H228 - H335 - H371
Precautionary statements: P210 - P240 - P241 - P260 - P264 - P308 + P311
GHS Symbol: GHS02+GHS07+GHS08
Signal word: Danger
RIDADR: UN1325
Transportation: Packing Group II

Description: Iodonitrotetrazolium chloride (INT) is used as an indicator dye. It is reduced by cellular activity to a red-colored formazan precipitate and used as a vital dye or indicator of cellular redox activity. INT is used in cell viability assays. Living cells with active metabolic processes can reduce INT to the red formazan. INT can also be used as an indicator of cellular redox activity. The reduction process reflects the cells' ability to transfer electrons, providing insights into their metabolic state. Reduction commonly results from the activity of dehydrogenases, although non-enzymatic electron transfer reactions can occur in the presence of an intermediate electron acceptor. This property of INT is used in various diagnostic assays (hematology/). INT is commonly used to measure the respiratory activity of microorganisms in a variety of contexts. The maximum absorption wavelength (?max) of INT (unreduced) is ~248nm. The reduced formazan (red) has a ?max ~490-520nm.
Smiles: IC(C=C1)=CC=C1[N+]2=NC(C3=CC=CC=C3)=NN2C4=CC=C([N+]([O-])=O)C=C4.[Cl-]
InChi Key: JORABGDXCIBAFL-UHFFFAOYSA-M
References: (1) J.D. Green & H.T. Narahara, J. Histochem. Cytochem. 28, 408 (1980) , (2) J.J. Podczasy & R. Wei, BBRC 150, 1294 (1988) , (3) M. Kamburova, et al., Talanta 40, 707 (1993) , (4) P. Munujos, et al., Anal. Biochem. 212, 506 (1993) , (5) M.V. Berridge, et al., Biotechnol. Ann. Rev. 11, 127 (2005) , (6) R.W. Sabnis, Handbook of biological dyes and stains (2010) , (7) Y. Liu, et al., Infect. Drug Resist. 13, 2593 (2020) , (8) E. Song, et al., ACS Appl. Mater. Interfaces 14, 38153 (2022)
InChi: InChI=1S/C19H13IN5O2.ClH/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27,/h1-13H,1H/q+1,/p-1

Iodonitrotetrazolium chloride (INT) is used as an indicator dye. It is reduced by cellular activity to a red-colored formazan precipitate and used as a vital dye or indicator of cellular redox activity. INT is used in cell viability assays. Living cells with active metabolic processes can reduce INT to the red formazan. INT can also be used as an indicator of cellular redox activity. The reduction process reflects the cells’ ability to transfer electrons, providing insights into their metabolic state. Reduction commonly results from the activity of dehydrogenases, although non-enzymatic electron transfer reactions can occur in the presence of an intermediate electron acceptor. This property of INT is used in various diagnostic assays (hematology/). INT is commonly used to measure the respiratory activity of microorganisms in a variety of contexts. The maximum absorption wavelength (?max) of INT (unreduced) is ~248nm. The reduced formazan (red) has a ?max ~490-520nm.

SKU: I0007 Category: Non Fluorescent Detection & Chromogene Detection

Additional information

Additional information

Synonyms	p-Iodonitrotetrazolium Violet, INT, 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride, NSC 27620
Purity	≥97% (TLC)
Appearance	White, dark yellow to dark orange powder
CAS-Number	146-68-9
Molecular Formula	C19H13CIN5O2
Molecular Weight	505.7
Identity	1H-NMR
Solvents	methanol (10mg/ml), ethanol (30mg/ml) or water (10mg/ml)
Melting Point	238-250 °C (dec.)
Smiles	IC(C=C1)=CC=C1[N+]2=NC(C3=CC=CC=C3)=NN2C4=CC=C([N+]([O-])=O)C=C4.[Cl-]
InChi Key	JORABGDXCIBAFL-UHFFFAOYSA-M
Shipping	AMBIENT
Short Term Storage	RT
Long Term Storage	+4°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at +4°C.
Hazard statements	H228 – H335 – H371
Precautionary statements	P210 – P240 – P241 – P260 – P264 – P308 + P311
GHS Symbol	GHS02+GHS07+GHS08
Signal word	Danger
RIDADR	UN1325
Transportation	Packing Group II
References	(1) J.D. Green & H.T. Narahara, J. Histochem. Cytochem. 28, 408 (1980), (2) J.J. Podczasy & R. Wei, BBRC 150, 1294 (1988), (3) M. Kamburova, et al., Talanta 40, 707 (1993), (4) P. Munujos, et al., Anal. Biochem. 212, 506 (1993), (5) M.V. Berridge, et al., Biotechnol. Ann. Rev. 11, 127 (2005), (6) R.W. Sabnis, Handbook of biological dyes and stains (2010), (7) Y. Liu, et al., Infect. Drug Resist. 13, 2593 (2020), (8) E. Song, et al., ACS Appl. Mater. Interfaces 14, 38153 (2022)
InChi	InChI=1S/C19H13IN5O2.ClH/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27,/h1-13H,1H/q+1,/p-1
Quantity	1 g, 5 g, Bulk
cdx-Solubility (color)	Yellow to yellow-green
cdx-Solubility (turbidity)	clear
cdx-4 mg/ml in water	Solubility (method)