IPB

SKU: I0004

Category: Non Fluorescent Detection & Chromogene Detection

Synonyms: 4-[3-(4-Iodophenyl)-2-(2,4-dinitrophenyl)-2H-5-tetrazolio]-1,3-benzenedisulfonate sodium salt; 161617-45-4
CAS-Number: C₁₉H₁₀IN₆NaO₁₀S₂
Molecular Formula: 696.34
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥90% (HPLC)
Identity: 1H-NMR
Appearance: Pale yellow powder

Properties

Solvents: chloroform

Short Term Storage: +4°C
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.
Handling Advice: Protect from light and moisture.
Long Term Storage: -20°C
Shipping: AMBIENT

Transportation: Not dangerous goods

Description: 4-[3-(4-iodophenyl)-2-(2,4-dinitrophenyl)-2H-5-tetrazolio]-1,3-benzenedisulfonate sodium salt (IPB) have found application in clinical assays and cell proliferation assays that are based on a spectrophotometric measurement of dehydrogenase activities of cells. Disulfonated tetrazolium salt IPB, that produces a highly water-soluble formazan dye that proved to be extremely useful as a cell-viability indicator. IPB is reduced by reduced nicotinamide adenine dinucleotide (NADH) to formazan in the presence of a redox mediator such as 1-methoxy PMS, which provides the basis to specrophotometric assays of NADH.
InChi: InChI=1S/C19H11IN6O10S2.Na/c20-11-1-3-12(4-2-11)23-21-19(15-7-6-14(37(31,32)33)10-18(15)38(34,35)36)22-24(23)16-8-5-13(25(27)28)9-17(16)26(29)30
References: (1) T. Mosmann: Immun. Meth. 65, 55 (1983) , (2) M.M. Nachlas et al.: Anal. Biochem. 1, 317 (1960)
InChi Key: LKVNWZJLLRMMAS-UHFFFAOYSA-M
Smiles: [Na+].[O-][N+](=O)C1=CC=C(N2N=C(N=[N+]2C2=CC=C(I)C=C2)C2=CC=C(C=C2S([O-])(=O)=O)S([O-])(=O)=O)C(=C1)[N+]([O-])=O

4-[3-(4-iodophenyl)-2-(2,4-dinitrophenyl)-2H-5-tetrazolio]-1,3-benzenedisulfonate sodium salt (IPB) have found application in clinical assays and cell proliferation assays that are based on a spectrophotometric measurement of dehydrogenase activities of cells. Disulfonated tetrazolium salt IPB, that produces a highly water-soluble formazan dye that proved to be extremely useful as a cell-viability indicator. IPB is reduced by reduced nicotinamide adenine dinucleotide (NADH) to formazan in the presence of a redox mediator such as 1-methoxy PMS, which provides the basis to specrophotometric assays of NADH.

SKU: I0004 Category: Non Fluorescent Detection & Chromogene Detection

Additional information

Additional information

Synonyms	4-[3-(4-Iodophenyl)-2-(2,4-dinitrophenyl)-2H-5-tetrazolio]-1,3-benzenedisulfonate sodium salt
Purity	≥90% (HPLC)
Appearance	Pale yellow powder
CAS-Number	161617-45-4
Molecular Formula	C19H10IN6NaO10S2
Molecular Weight	696.34
Identity	1H-NMR
Solvents	chloroform
Smiles	[Na+].[O-][N+](=O)C1=CC=C(N2N=C(N=[N+]2C2=CC=C(I)C=C2)C2=CC=C(C=C2S([O-])(=O)=O)S([O-])(=O)=O)C(=C1)[N+]([O-])=O
InChi Key	LKVNWZJLLRMMAS-UHFFFAOYSA-M
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Transportation	Not dangerous goods
References	(1) T. Mosmann: Immun. Meth. 65, 55 (1983), (2) M.M. Nachlas et al.: Anal. Biochem. 1, 317 (1960)
InChi	InChI=1S/C19H11IN6O10S2.Na/c20-11-1-3-12(4-2-11)23-21-19(15-7-6-14(37(31,32)33)10-18(15)38(34,35)36)22-24(23)16-8-5-13(25(27)28)9-17(16)26(29)30
Quantity	250 mg, 1 g, Bulk