Specifications
- Purity
- ≥98% (HPLC)
- Appearance
- Deep blue, violet crystals
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO or water
Available from stock
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Description
- Derivative of JC-1 useful for determining mitochondrial membrane potential by flow cytometry, fluorescence microscopy and in microplate-based fluorescent assays. Accumulates in mitochondria, selectively generating an orange J-aggregate emission profile (570 nm) in healthy cells. Upon cell injury, as membrane potential decreases, JC-10 monomers are generated, resulting in a shift to green emission (520 nm). Shows improved solubility compared to JC-1 in aqueous media and an ability to detect subtler changes in mitochondrial membrane potential loss. Spectral data: ?ex 510 nm| ?em 525.
- References
- (1) L. Donaghy, et al.: PLoS One 7, e46594 (2012) , (2) C.C. Chen, et al.: Drug Des. Dev. Ther. 8, 459 (2014) , (3) S. Yokokura, et al.: BMC Cancer 26, 882 (2014) , (4) C. Filippi, et al.: Nanotoxicology 9, 126 (2015)
Derivative of JC-1 useful for determining mitochondrial membrane potential by flow cytometry, fluorescence microscopy and in microplate-based fluorescent assays. Accumulates in mitochondria, selectively generating an orange J-aggregate emission profile (570 nm) in healthy cells. Upon cell injury, as membrane potential decreases, JC-10 monomers are generated, resulting in a shift to green emission (520 nm). Shows improved solubility compared to JC-1 in aqueous media and an ability to detect subtler changes in mitochondrial membrane potential loss. Spectral data: ?ex 510 nm| ?em 525.
Additional information
| Purity | ≥98% (HPLC) |
|---|---|
| Appearance | Deep blue, violet crystals |
| CAS-Number | n/a |
| Identity | 1H-NMR |
| Solvents | DMSO or water |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| References | (1) L. Donaghy, et al.: PLoS One 7, e46594 (2012), (2) C.C. Chen, et al.: Drug Des. Dev. Ther. 8, 459 (2014), (3) S. Yokokura, et al.: BMC Cancer 26, 882 (2014), (4) C. Filippi, et al.: Nanotoxicology 9, 126 (2015) |
| Quantity | 1 mg, 5 mg, Bulk |
