- ≥98% (HPLC)
- Deep blue, violet crystals
- DMSO or water
Category: Fluorescent Detection
Derivative of JC-1 useful for determining mitochondrial membrane potential by flow cytometry, fluorescence microscopy and in microplate-based fluorescent assays. Accumulates in mitochondria, selectively generating an orange J-aggregate emission profile (570 nm) in healthy cells. Upon cell injury, as membrane potential decreases, JC-10 monomers are generated, resulting in a shift to green emission (520 nm). Shows improved solubility compared to JC-1 in aqueous media and an ability to detect subtler changes in mitochondrial membrane potential loss. Spectral data: ?ex 510 nm| ?em 525.
Deep blue, violet crystals
DMSO or water
|Short Term Storage|
|Long Term Storage|
Protect from light and moisture.
|Use / Stability|
Stable for at least 2 years after receipt when stored at -20°C.
(1) L. Donaghy, et al.: PLoS One 7, e46594 (2012), (2) C.C. Chen, et al.: Drug Des. Dev. Ther. 8, 459 (2014), (3) S. Yokokura, et al.: BMC Cancer 26, 882 (2014), (4) C. Filippi, et al.: Nanotoxicology 9, 126 (2015)
1 mg, 5 mg, Bulk