Available from stock

L-Leucine-7-amido-4-methylcoumarin hydrochloride

SKU: L0003

Categories: Fluorescent Detection, Fluorescent Detection

Synonyms: H-Leu-AMC hydrochloride; 62480-44-8
CAS-Number: C₁₆H₂₀N₂O₃ . HCl
Molecular Formula: 324.8
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥99% (HPLC)
Appearance: White to off-white powder
Identity: 1H-NMR

Properties

Solvents: methanol
Optical Activity: [α]20/D +62.0±2.0°, c = 1% in methanol
Fluorescence: λex 325 nm, λem 397 nm in 0.1 M Tris pH 8.0, λex 380 nm, λem 440 nm (after digestion with leucine aminopeptidase)

Documentation

Safety Data Sheet (SDS): CDX L0003 MSDS_Vs1.2.pdf

Shipping: AMBIENT
Short Term Storage: +4°C
Long Term Storage: -20°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.

Transportation: Not dangerous goods

Description: Fluorogenic substrate for leucine aminopeptidase. Sensitivity of fluorescence detection is greatly increased over other methods such as 4-Methoxy-naphthylamide (pNA) assays.
Smiles: Cl.CC(C)C[C@H](N)C(=O)NC1=CC=C2C(C)=CC(=O)OC2=C1
InChi Key: VCRXITKKWBOQRZ-ZOWNYOTGSA-N
References: (1) R. Zaccone et al.: Marine Environm. Res. 54(1), 1 (2002) , (2) L. Stepaniak: J. Food Prot. 63(10), 1447 (2000) , (3) Y. Kanaoka et al.: Chem. Pharm. Bull. 33(4), 1721 (1985) , (4) Y. Kanaoka et al.: Chem. Pharm. Bull. 25, 362 (1977)
InChi: InChI=1S/C16H20N2O3.ClH/c1-9(2)6-13(17)16(20)18-11-4-5-12-10(3)7-15(19)21-14(12)8-11

Fluorogenic substrate for leucine aminopeptidase. Sensitivity of fluorescence detection is greatly increased over other methods such as 4-Methoxy-naphthylamide (pNA) assays.

SKU: L0003 Categories: Fluorescent Detection, Fluorescent Detection

Additional information

Additional information

Synonyms	H-Leu-AMC hydrochloride
Purity	≥99% (HPLC)
Appearance	White to off-white powder
CAS-Number	62480-44-8
Molecular Formula	C16H20N2O3 . HCl
Molecular Weight	324.8
Identity	1H-NMR
Solvents	methanol
Optical Activity	[α]20/D +62.0±2.0°, c = 1% in methanol
Fluorescence	λex 325 nm, λem 397 nm in 0.1 M Tris pH 8.0, λex 380 nm, λem 440 nm (after digestion with leucine aminopeptidase)
Smiles	Cl.CC(C)C[C@H](N)C(=O)NC1=CC=C2C(C)=CC(=O)OC2=C1
InChi Key	VCRXITKKWBOQRZ-ZOWNYOTGSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Transportation	Not dangerous goods
References	(1) R. Zaccone et al.: Marine Environm. Res. 54(1), 1 (2002), (2) L. Stepaniak: J. Food Prot. 63(10), 1447 (2000), (3) Y. Kanaoka et al.: Chem. Pharm. Bull. 33(4), 1721 (1985), (4) Y. Kanaoka et al.: Chem. Pharm. Bull. 25, 362 (1977)
InChi	InChI=1S/C16H20N2O3.ClH/c1-9(2)6-13(17)16(20)18-11-4-5-12-10(3)7-15(19)21-14(12)8-11
Quantity	10 mg, 50 mg, Bulk