Specifications
- Purity
- ≥97% (Titration)
- Appearance
- White to light yellow powder
- Identity
- 1H-NMR
Properties
- Solvents
- water (50 mg/ml), NaoH
- Melting Point
- >300 °C(lit.)
Available from stock
Luminol
- SKU
- L0012
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 3-Aminophthalhydrazide , 5-Amino-2,3-dihydro-1,4-phthalazinedione , NSC5064
- 521-31-3
- CAS-Number
- C8H7N3O2
- Molecular Formula
- 177.16
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- RT
- Long Term Storage
- +4°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at +4°C.
- Hazard statements
- H315-H319-H335
- Precautionary statements
- P261-P305 + P351 + P338
- GHS Symbol
- GHS07
- Signal word
- Warning
- Transportation
- Not dangerous goods
- Description
- Very efficient and commonly used chemiluminiscent compound in many applications. Cyclic diacyl hydrazide that exhibits chemiluminescence upon oxidation. Oxidizing agents capable of initiating this particular reaction are nitrogen dioxide, PAN, hydrogen peroxide, ozone and other atmospheric oxidants. As an example, HRP combined with hydrogen peroxide as an activator, causes luminescent peroxidation of luminol. This reaction can be enhanced by certain phenol derivatives, such as p-substituted phenols. Luminol can also be oxidized and chemiluminesce, by compounds containing iron, copper, gold, and cyanide. Luminol is a reagent for the generation of chemiluminescence in biological assays including nitric oxide and myeloperoxidase assays or in fornensic blood tests. Spectral data: λex=355nm, λem=411nm.
- Smiles
- O=C1C2=C(C(N)=CC=C2)C(NN1)=O
- InChi Key
- HWYHZTIRURJOHG-UHFFFAOYSA-N
- References
- (1) M.J. Cormier & P.M. Prichard, J. Biol. Chem. 243, 4706 (1968) , (2) G.H.G. Thorpe, et al., Clin. Chem. 31, 1335 (1985) , (3) T.I. Quickenden & J.I. Creamer, Luminescence 16, 295 (2001) , (4) A. Fan, et al., Anal. Sci. 25, 5877 (2009) , (5) Y.A. Vladimirov, et al., Biochem. 74, 1545 (2009)
- InChi
- InChI=1S/C8H7N3O2/c9-5-3-1-2-4-6(5)8(13)11-10-7(4)12/h1-3H,9H2,(H,10,12)(H,11,13)
Very efficient and commonly used chemiluminiscent compound in many applications. Cyclic diacyl hydrazide that exhibits chemiluminescence upon oxidation. Oxidizing agents capable of initiating this particular reaction are nitrogen dioxide, PAN, hydrogen peroxide, ozone and other atmospheric oxidants. As an example, HRP combined with hydrogen peroxide as an activator, causes luminescent peroxidation of luminol. This reaction can be enhanced by certain phenol derivatives, such as p-substituted phenols. Luminol can also be oxidized and chemiluminesce, by compounds containing iron, copper, gold, and cyanide. Luminol is a reagent for the generation of chemiluminescence in biological assays including nitric oxide and myeloperoxidase assays or in fornensic blood tests. Spectral data: λex=355nm, λem=411nm.
Additional information
| Synonyms | 3-Aminophthalhydrazide, 5-Amino-2,3-dihydro-1,4-phthalazinedione, NSC5064 |
|---|---|
| Purity | ≥97% (Titration) |
| Appearance | White to light yellow powder |
| CAS-Number | 521-31-3 |
| Molecular Formula | C8H7N3O2 |
| Molecular Weight | 177.16 |
| Identity | 1H-NMR |
| Solvents | water (50 mg/ml), NaoH |
| Melting Point | >300 °C(lit.) |
| Smiles | O=C1C2=C(C(N)=CC=C2)C(NN1)=O |
| InChi Key | HWYHZTIRURJOHG-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | RT |
| Long Term Storage | +4°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Hazard statements | H315-H319-H335 |
| Precautionary statements | P261-P305 + P351 + P338 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) M.J. Cormier & P.M. Prichard, J. Biol. Chem. 243, 4706 (1968), (2) G.H.G. Thorpe, et al., Clin. Chem. 31, 1335 (1985), (3) T.I. Quickenden & J.I. Creamer, Luminescence 16, 295 (2001), (4) A. Fan, et al., Anal. Sci. 25, 5877 (2009), (5) Y.A. Vladimirov, et al., Biochem. 74, 1545 (2009) |
| InChi | InChI=1S/C8H7N3O2/c9-5-3-1-2-4-6(5)8(13)11-10-7(4)12/h1-3H,9H2,(H,10,12)(H,11,13) |
| Quantity | 25 g, 100 g, Bulk |
