Specifications
- Purity
- ≥90% (HPLC)
- Appearance
- Orange to brown solid
- Identity
- 1H-NMR
Properties
- Solvents
- DMF
- Fluorescence
- λex 492 nm, λem 518 nm in 0.1 M Tris pH 9.0, 2-mercaptoethanol
Documentation
- Safety Data Sheet (SDS)
- CDX F0004 MSDS.pdf
Available from stock
N-(5-Fluoresceinyl)-maleinimide
- SKU
- F0004
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- N-(5-Fluoresceinyl)-maleimide , Fluorescein-5-maleimide , 1-(3',6'-Dihydroxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthen]-5-yl)-1H-pyrrole-2,5-dione
- 75350-46-8
- CAS-Number
- C24H13NO7
- Molecular Formula
- 427.36
- Molecular Weight
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Hazard statements
- H302
- GHS Symbol
- GHS07
- Signal word
- Warning
- Transportation
- Not dangerous goods
- Description
- Green fluorescent thiol-reactive dye (Ex/Em: 494/519nm) for bioconjugation, widely used as protein and peptide fluorescent labeling reagent, easily conjugating fluorescein. The reaction requires very mild condition and is highly specific. The maleimide group reacts optimally with sulfhydryl groups on cysteine side chains at pH 7, forming a stable thioether bond. The compound can be used to label proteins for the detection of conformational changes, assembly of multisubunit complexes, and ligand-binding processes.
- Smiles
- OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(=C3)N1C(=O)C=CC1=O)C1=C(O2)C=C(O)C=C1
- InChi Key
- AYDAHOIUHVUJHQ-UHFFFAOYSA-N
- References
- (1) Anal. Lett. 18, 393 (1985) , (2) Biochem.: 30, 2113 (1991)
- InChi
- InChI=1S/C24H13NO7/c26-13-2-5-17-19(10-13)31-20-11-14(27)3-6-18(20)24(17)16-4-1-12(9-15(16)23(30)32-24)25-21(28)7-8-22(25)29/h1-11,26-27H
Green fluorescent thiol-reactive dye (Ex/Em: 494/519nm) for bioconjugation, widely used as protein and peptide fluorescent labeling reagent, easily conjugating fluorescein. The reaction requires very mild condition and is highly specific. The maleimide group reacts optimally with sulfhydryl groups on cysteine side chains at pH 7, forming a stable thioether bond. The compound can be used to label proteins for the detection of conformational changes, assembly of multisubunit complexes, and ligand-binding processes.
Additional information
| Synonyms | N-(5-Fluoresceinyl)-maleimide, Fluorescein-5-maleimide, 1-(3',6'-Dihydroxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthen]-5-yl)-1H-pyrrole-2,5-dione |
|---|---|
| Purity | ≥90% (HPLC) |
| Appearance | Orange to brown solid |
| CAS-Number | 75350-46-8 |
| Molecular Formula | C24H13NO7 |
| Molecular Weight | 427.36 |
| Identity | 1H-NMR |
| Solvents | DMF |
| Fluorescence | λex 492 nm, λem 518 nm in 0.1 M Tris pH 9.0, 2-mercaptoethanol |
| Smiles | OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(=C3)N1C(=O)C=CC1=O)C1=C(O2)C=C(O)C=C1 |
| InChi Key | AYDAHOIUHVUJHQ-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Hazard statements | H302 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) Anal. Lett. 18, 393 (1985), (2) Biochem.: 30, 2113 (1991) |
| InChi | InChI=1S/C24H13NO7/c26-13-2-5-17-19(10-13)31-20-11-14(27)3-6-18(20)24(17)16-4-1-12(9-15(16)23(30)32-24)25-21(28)7-8-22(25)29/h1-11,26-27H |
| Quantity | 50 mg, 500 mg, Bulk |
