Available from stock

N-Aminofluorescein

SKU: A0936

Category: Fluorescent Detection

Synonyms: 2-Amino-3',6'-dihydroxyspiro[isoindole-3,9'-xanthene]-1-one, 2-Amino-3',6'-dihydroxyspiro[isoindoline-1,9'-xanthen]-3-one, NSC 374134; 98907-26-7
CAS-Number: C₂₀H₁₄N₂O₄
Molecular Formula: 346.34
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥98%
Appearance: Off white to slight yellow powder
Identity: 1H-NMR

Properties

Solvents: Soluble in DMSO (50mg/ml).

Shipping: AMBIENT
Short Term Storage: RT
Long Term Storage: +4°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at +4°C.

Hazard statements: H302-H315-H319
Precautionary statements: P501-P270-P264-P280-P302+P352-P337+P313-P305+P351+P338-P362+P364-P332+P313-P301+P312+P330
GHS Symbol: GHS07
Signal word: Warning
Transportation: Not dangerous goods

Description: N-Aminofluorescein is a fluorescein hydrazide with spiro ring structure and described as a highly selective and sensitive fluorescence probe for Cu2+. It absorbs maximally around 490–495 nm and emits bright green fluorescence near 515–520 nm, with a high quantum yield. It can be used for direct detection of Cu2+ in biological systems. Spectral Data: ?ex/em = 495/516nm. The presence of the exocyclic amino group in the structure, allows for selective chemical reactivity, making it suitable for use as a building block in the design of redox-sensitive probes, labeling reagents, and diagnostic sensors.
Smiles: O=C(C1=C2C=CC=C1)N(N)C32C4=CC=C(O)C=C4OC5=C3C=CC(O)=C5
InChi Key: CHYVTSCIBXXQJT-UHFFFAOYSA-N
References: (1) M. Adamczyk & J. Grote, Tetrahedr. Lett. 41, 807 (2000) , (2) X.-F. Yang, et al., Microchim. Acta 149, 123 (2005) , (3) X. Chen & H. Ma, Anal. Chim. Acta 575, 217 (2006) , (4) X.-F. Yang, et al., Anal. Chim. Acta 584, 95 (2007) , (5) T. Li, et al., Dyes Pigm. 88, 103 (2011) , (6) C. Shen, et al., Chem. Commun. 51, 6312 (2015) , (7) C. Cetintas, et al., Sens. Actuat. B: Chem. 234, 109 (2016) , (8) Y.L. Chen, et al., J. Luminescence 275, 120747 (2024) , (9) H.T. Zhi, et al., Bioorg. Chem. 147, 107421 (2024)
InChi: InChI=1S/C20H14N2O4/c21-22-19(25)13-3-1-2-4-14(13)20(22)15-7-5-11(23)9-17(15)26-18-10-12(24)6-8-16(18)20/h1-10,23-24H,21H2

N-Aminofluorescein is a fluorescein hydrazide with spiro ring structure and described as a highly selective and sensitive fluorescence probe for Cu2+. It absorbs maximally around 490–495 nm and emits bright green fluorescence near 515–520 nm, with a high quantum yield. It can be used for direct detection of Cu2+ in biological systems. Spectral Data: ?ex/em = 495/516nm. The presence of the exocyclic amino group in the structure, allows for selective chemical reactivity, making it suitable for use as a building block in the design of redox-sensitive probes, labeling reagents, and diagnostic sensors.

SKU: A0936 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	2-Amino-3',6'-dihydroxyspiro[isoindole-3,9'-xanthene]-1-one, 2-Amino-3',6'-dihydroxyspiro[isoindoline-1,9'-xanthen]-3-one, NSC 374134
Purity	≥98%
Appearance	Off white to slight yellow powder
CAS-Number	98907-26-7
Molecular Formula	C20H14N2O4
Molecular Weight	346.34
Identity	1H-NMR
Solvents	Soluble in DMSO (50mg/ml).
Smiles	O=C(C1=C2C=CC=C1)N(N)C32C4=CC=C(O)C=C4OC5=C3C=CC(O)=C5
InChi Key	CHYVTSCIBXXQJT-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	RT
Long Term Storage	+4°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at +4°C.
Hazard statements	H302-H315-H319
Precautionary statements	P501-P270-P264-P280-P302+P352-P337+P313-P305+P351+P338-P362+P364-P332+P313-P301+P312+P330
GHS Symbol	GHS07
Signal word	Warning
Transportation	Not dangerous goods
References	(1) M. Adamczyk & J. Grote, Tetrahedr. Lett. 41, 807 (2000), (2) X.-F. Yang, et al., Microchim. Acta 149, 123 (2005), (3) X. Chen & H. Ma, Anal. Chim. Acta 575, 217 (2006), (4) X.-F. Yang, et al., Anal. Chim. Acta 584, 95 (2007), (5) T. Li, et al., Dyes Pigm. 88, 103 (2011), (6) C. Shen, et al., Chem. Commun. 51, 6312 (2015), (7) C. Cetintas, et al., Sens. Actuat. B: Chem. 234, 109 (2016), (8) Y.L. Chen, et al., J. Luminescence 275, 120747 (2024), (9) H.T. Zhi, et al., Bioorg. Chem. 147, 107421 (2024)
InChi	InChI=1S/C20H14N2O4/c21-22-19(25)13-3-1-2-4-14(13)20(22)15-7-5-11(23)9-17(15)26-18-10-12(24)6-8-16(18)20/h1-10,23-24H,21H2
Quantity	50 mg, 100 mg, Bulk