Available from stock

N’-Octadecylfluorescein-5-thiourea

SKU: O0016

Category: Fluorescent Detection

Synonyms: AF18, F 18 Fluorescein, N-(Fluorescein-5-thiocarbamoyl)octadecylamine; 65603-18-1
CAS-Number: C₃₉H₅₀N₂O₅S
Molecular Formula: 658.89
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥95% (TLC)
Appearance: Orange solid
Identity: 1H-NMR

Properties

Solvents: Soluble in DMSO or DMF.
UV / Vis: 497 ± 3 nm in 0.2M KOH/MeOH
Fluorescence: 518 ± 4 nm in 0.2M KOH/MeOH

Shipping: AMBIENT
Short Term Storage: RT
Long Term Storage: +4°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at +4°C.

Hazard statements: H315-H319-H335
Precautionary statements: P261-P305+P351+P338
GHS Symbol: GHS07
Signal word: Warning
Transportation: Not dangerous goods

Description: N'-Octadecylfluorescein-5-thiourea (AF18) is fluorescent membrane probes. The fluorescent probes was synthesized by the covalent linkage of fluorescein to a saturated hydrocarbon chain, 18 carbon atoms long. The hydrocarbon chains cause the probes to partition into cell membranes. AF18 remains in the membrane of the target cells even when they are killed. The fluorescence spectra is similar to FITC. Spectral Data: λEx: 494nm. λEm: 517nm (PBS pH9) (Lit).
Smiles: O=C1OC2(C3=C(C=C(C=C3)O)OC4=C2C=CC(O)=C4)C5=C1C=C(NC(NCCCCCCCCCCCCCCCCCC)=S)C=C5
InChi Key: RFUZGLNSFGBBJW-UHFFFAOYSA-N
References: (1) P.M. Keller, et al.; J. Cell Sci. 28, 167 (1977) , (2) H. Horie, et al.; Brain Res. 411, 298 (1987) , (3) K. Radosevic, et al.; J. Immunol. Meth. 135, 81 (1990) , (4) C.A. Redman & J.R. Kusel; Parasitol. 113, 137 (1996) , (5) K. Takemoto, et al.; Biol. Cell 91, 5 (1999) , (6) S. Murgia, et al.; Langmuir 29, 6673 (2013)
InChi: InChI=1S/C39H50N2O5S/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-24-40-38(47)41-28-18-21-32-31(25-28)37(44)46-39(32)33-22-19-29(42)26-35(33)45-36-27-30(43)20-23-34(36)39/h18-23,25-27,42-43H,2-17,24H2,1H3,(H2,40,41,47)

N’-Octadecylfluorescein-5-thiourea (AF18) is fluorescent membrane probes. The fluorescent probes was synthesized by the covalent linkage of fluorescein to a saturated hydrocarbon chain, 18 carbon atoms long. The hydrocarbon chains cause the probes to partition into cell membranes. AF18 remains in the membrane of the target cells even when they are killed. The fluorescence spectra is similar to FITC. Spectral Data: λEx: 494nm. λEm: 517nm (PBS pH9) (Lit).

SKU: O0016 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	AF18, F 18 Fluorescein, N-(Fluorescein-5-thiocarbamoyl)octadecylamine
Purity	≥95% (TLC)
Appearance	Orange solid
CAS-Number	65603-18-1
Molecular Formula	C39H50N2O5S
Molecular Weight	658.89
Identity	1H-NMR
Solvents	Soluble in DMSO or DMF.
UV / Vis	497 ± 3 nm in 0.2M KOH/MeOH
Fluorescence	518 ± 4 nm in 0.2M KOH/MeOH
Smiles	O=C1OC2(C3=C(C=C(C=C3)O)OC4=C2C=CC(O)=C4)C5=C1C=C(NC(NCCCCCCCCCCCCCCCCCC)=S)C=C5
InChi Key	RFUZGLNSFGBBJW-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	RT
Long Term Storage	+4°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at +4°C.
Hazard statements	H315-H319-H335
Precautionary statements	P261-P305+P351+P338
GHS Symbol	GHS07
Signal word	Warning
Transportation	Not dangerous goods
References	(1) P.M. Keller, et al.; J. Cell Sci. 28, 167 (1977), (2) H. Horie, et al.; Brain Res. 411, 298 (1987), (3) K. Radosevic, et al.; J. Immunol. Meth. 135, 81 (1990), (4) C.A. Redman & J.R. Kusel; Parasitol. 113, 137 (1996), (5) K. Takemoto, et al.; Biol. Cell 91, 5 (1999), (6) S. Murgia, et al.; Langmuir 29, 6673 (2013)
InChi	InChI=1S/C39H50N2O5S/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-24-40-38(47)41-28-18-21-32-31(25-28)37(44)46-39(32)33-22-19-29(42)26-35(33)45-36-27-30(43)20-23-34(36)39/h18-23,25-27,42-43H,2-17,24H2,1H3,(H2,40,41,47)
Quantity	100 mg, Bulk