Specifications
- Purity
- ≥98% (HPLC)
- Identity
- 1H-NMR
- Appearance
- Orange powder
Properties
- Solvents
- DMSO, DMF
Available from stock
NBD-X SE
- SKU
- N0045
Category: Fluorescent Detection
- Synonyms
- NBD-X, SE , NBD-X N-succinimidyl ester , NBD-C6 SE , 6-(7-Nitrobenzofurazan-4-ylamino)hexanoic acid NHS ester
- 145195-58-0
- CAS-Number
- C16H17N5O7
- Molecular Formula
- 391.33
- Molecular Weight
- Short Term Storage
- +4°C
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Handling Advice
- Protect from light and moisture.
- Long Term Storage
- -20°C
- Shipping
- AMBIENT
- Transportation
- Not dangerous goods
- Description
- NBD-X, SE is an excellent amino-reactive building block that can be used to prepare peptide conjugates and other bioconjugates/biopolymers. It has much higher conjugation efficiency than NBD-Cl and NBD-F. This amine-reactive NBD derivative, containing a convenient succinimidyl ester, can be used to develop environmentally sensitive bioconjugates. NBD is a functional analog of the dinitrophenyl hapten, and its fluorescence is quenched upon binding to anti-dinitrophenyl (anti-DNP) antibodies. The X-spacer separates the fluorescent dye from the protein allowing greater sensitivity and reduced perturbation effects on protein labeling. Spectral properties: λEx=460, λEm=540.
- References
- (1) C. Sherrill, et al., Biochemistry 34, 3553 (1995) , (2) B.K. Nunnally, et al., Anal. Chem. 69, 2392 (1997) , (3) J.T. Elliott & G.D. Prestwich, Bioconjug. Chem. 11, 832 (2000) , (4) S. Ozaki, et al., PNAS 97, 11286 (2000) , (5) A. Karlstrom, et al., Anal. Biochem. 295, 22 (2001) , (6) F. Eisele, et al., Chem. Eur. J. 8, 3362 (2002) , (7) L. Silva, et al., J. Photochem. Photobiol. B 72, 17 (2003) , (8) J.M. Bergen, et al., Bioconjug. Chem. 19, 377 (2008)
- Smiles
- O=C(ON1C(CCC1=O)=O)CCCCCNC2=CC=C([N+]([O-])=O)C3=NON=C32
NBD-X, SE is an excellent amino-reactive building block that can be used to prepare peptide conjugates and other bioconjugates/biopolymers. It has much higher conjugation efficiency than NBD-Cl and NBD-F. This amine-reactive NBD derivative, containing a convenient succinimidyl ester, can be used to develop environmentally sensitive bioconjugates. NBD is a functional analog of the dinitrophenyl hapten, and its fluorescence is quenched upon binding to anti-dinitrophenyl (anti-DNP) antibodies. The X-spacer separates the fluorescent dye from the protein allowing greater sensitivity and reduced perturbation effects on protein labeling. Spectral properties: λEx=460, λEm=540.
Additional information
| Synonyms | NBD-X, SE, NBD-X N-succinimidyl ester, NBD-C6 SE, 6-(7-Nitrobenzofurazan-4-ylamino)hexanoic acid NHS ester |
|---|---|
| Purity | ≥98% (HPLC) |
| Appearance | Orange powder |
| CAS-Number | 145195-58-0 |
| Molecular Formula | C16H17N5O7 |
| Molecular Weight | 391.33 |
| Identity | 1H-NMR |
| Solvents | DMSO, DMF |
| Smiles | O=C(ON1C(CCC1=O)=O)CCCCCNC2=CC=C([N+]([O-])=O)C3=NON=C32 |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Transportation | Not dangerous goods |
| References | (1) C. Sherrill, et al., Biochemistry 34, 3553 (1995), (2) B.K. Nunnally, et al., Anal. Chem. 69, 2392 (1997), (3) J.T. Elliott & G.D. Prestwich, Bioconjug. Chem. 11, 832 (2000), (4) S. Ozaki, et al., PNAS 97, 11286 (2000), (5) A. Karlstrom, et al., Anal. Biochem. 295, 22 (2001), (6) F. Eisele, et al., Chem. Eur. J. 8, 3362 (2002), (7) L. Silva, et al., J. Photochem. Photobiol. B 72, 17 (2003), (8) J.M. Bergen, et al., Bioconjug. Chem. 19, 377 (2008) |
| Quantity | 25 mg, Bulk |
