NBD-X SE

SKU: N0045

Category: Fluorescent Detection

Synonyms: NBD-X, SE , NBD-X N-succinimidyl ester , NBD-C6 SE , 6-(7-Nitrobenzofurazan-4-ylamino)hexanoic acid NHS ester; 145195-58-0
CAS-Number: C₁₆H₁₇N₅O₇
Molecular Formula: 391.33
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥98% (HPLC)
Identity: 1H-NMR
Appearance: Orange powder

Properties

Solvents: DMSO, DMF

Short Term Storage: +4°C
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.
Handling Advice: Protect from light and moisture.
Long Term Storage: -20°C
Shipping: AMBIENT

Transportation: Not dangerous goods

Description: NBD-X, SE is an excellent amino-reactive building block that can be used to prepare peptide conjugates and other bioconjugates/biopolymers. It has much higher conjugation efficiency than NBD-Cl and NBD-F. This amine-reactive NBD derivative, containing a convenient succinimidyl ester, can be used to develop environmentally sensitive bioconjugates. NBD is a functional analog of the dinitrophenyl hapten, and its fluorescence is quenched upon binding to anti-dinitrophenyl (anti-DNP) antibodies. The X-spacer separates the fluorescent dye from the protein allowing greater sensitivity and reduced perturbation effects on protein labeling. Spectral properties: λEx=460, λEm=540.
References: (1) C. Sherrill, et al., Biochemistry 34, 3553 (1995) , (2) B.K. Nunnally, et al., Anal. Chem. 69, 2392 (1997) , (3) J.T. Elliott & G.D. Prestwich, Bioconjug. Chem. 11, 832 (2000) , (4) S. Ozaki, et al., PNAS 97, 11286 (2000) , (5) A. Karlstrom, et al., Anal. Biochem. 295, 22 (2001) , (6) F. Eisele, et al., Chem. Eur. J. 8, 3362 (2002) , (7) L. Silva, et al., J. Photochem. Photobiol. B 72, 17 (2003) , (8) J.M. Bergen, et al., Bioconjug. Chem. 19, 377 (2008)
Smiles: O=C(ON1C(CCC1=O)=O)CCCCCNC2=CC=C([N+]([O-])=O)C3=NON=C32

NBD-X, SE is an excellent amino-reactive building block that can be used to prepare peptide conjugates and other bioconjugates/biopolymers. It has much higher conjugation efficiency than NBD-Cl and NBD-F. This amine-reactive NBD derivative, containing a convenient succinimidyl ester, can be used to develop environmentally sensitive bioconjugates. NBD is a functional analog of the dinitrophenyl hapten, and its fluorescence is quenched upon binding to anti-dinitrophenyl (anti-DNP) antibodies. The X-spacer separates the fluorescent dye from the protein allowing greater sensitivity and reduced perturbation effects on protein labeling. Spectral properties: λEx=460, λEm=540.

SKU: N0045 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	NBD-X, SE, NBD-X N-succinimidyl ester, NBD-C6 SE, 6-(7-Nitrobenzofurazan-4-ylamino)hexanoic acid NHS ester
Purity	≥98% (HPLC)
Appearance	Orange powder
CAS-Number	145195-58-0
Molecular Formula	C16H17N5O7
Molecular Weight	391.33
Identity	1H-NMR
Solvents	DMSO, DMF
Smiles	O=C(ON1C(CCC1=O)=O)CCCCCNC2=CC=C([N+]([O-])=O)C3=NON=C32
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Transportation	Not dangerous goods
References	(1) C. Sherrill, et al., Biochemistry 34, 3553 (1995), (2) B.K. Nunnally, et al., Anal. Chem. 69, 2392 (1997), (3) J.T. Elliott & G.D. Prestwich, Bioconjug. Chem. 11, 832 (2000), (4) S. Ozaki, et al., PNAS 97, 11286 (2000), (5) A. Karlstrom, et al., Anal. Biochem. 295, 22 (2001), (6) F. Eisele, et al., Chem. Eur. J. 8, 3362 (2002), (7) L. Silva, et al., J. Photochem. Photobiol. B 72, 17 (2003), (8) J.M. Bergen, et al., Bioconjug. Chem. 19, 377 (2008)
Quantity	25 mg, Bulk